Sumbawagen iGEM Team
Team:Sumbawagen/Protocols
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- | < | + | <p style="padding-bottom: 0pt; padding-top: 0pt; " class="Free_Form">Antibiotics</p> |
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+ | <p style="padding-bottom: 0pt; padding-top: 0pt; " class="Free_Form"> LB Medium Bacterial Agar Sterile Water</p> | ||
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- | <br> | + | <p style="padding-top: 0pt; " class="Free_Form">PROTOCOL<br /></p> |
+ | <p class="Free_Form">1.Mix Aquades and Agar<br /></p> | ||
+ | <p class="Free_Form">2.Pour mixture into Tube (Liquid Medium) and Bottle (Solid Medium)<br /></p> | ||
+ | <p class="Free_Form">3.Close the tube with cotton and bottle with the bottle cover<br /></p> | ||
+ | <p class="Free_Form">4.Wrap the tube and the bottle that we have closed using aluminium foil<br /></p> | ||
+ | <p class="Free_Form">5.Sterilise into the pressure cooker,put 1100 ml of water into the pressure cooker<br /></p> | ||
+ | <p class="Free_Form">6.Wait for 45 minutes<br /></p> | ||
+ | <p class="Free_Form">7.Take it out and let it warm<br /></p> | ||
+ | <p class="Free_Form">8.For the liquid medium put it in refrigator<br /></p> | ||
+ | <p class="Free_Form">9.For a solid medium add antibiotic<br /></p> | ||
+ | <p class="Free_Form">10.Pour into LB plates,do it in the laminar<br /></p> | ||
+ | <p class="Free_Form">11.Wait until the agar getting solid,down the position of agar plates and wair for 24 hours<br /></p> | ||
+ | <p class="Free_Form">12.Your agar is ready to use<br /></p> | ||
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Revision as of 15:29, 26 September 2013