Team:TU Darmstadt/labbook

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Revision as of 19:03, 4 October 2013





Labbook | Materials | Protocols


Detection construct


The assembly of our detection construct proved to be tricky. Not only the traditional cloning approach failed but also the assembly using synthesized gBlocks delivered no results. We finally turned to an In-Fusion cloning approach to assemble our construct. Visit our lab book to read more about our work flow.



Labbookdetection Visit Our Labbook for the detection construct

Fluorescence proteins


The genes for our FRET pair, mKate and LssmOrange, were isolated by PCR, cloned into the biobrick vector and sequenced afterwards. The genes were also cloned into a commercially available expression vector in order to characterize the fluorescent proteins. Visit our lab book to read more about our work flow.



mkate mkateactive



Labbookbiobricks Visit Our Labbook for the fluorescence proteins

Safety


The construction of the pDawn vector from gBlocks failed.



Labbookbiobricks Visit our lab book for the safety construct
                

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