Team:TU Darmstadt/materials/Media

From 2013.igem.org

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1.7-DYT (recipe for 500 mL)
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<li><a href="http://ecoliwiki.net/colipedia/index.php/TOP10"><font size="3" color="#F0F8FF" face="Arial regular"><b>TOP10(homehold)</b></font></a></li>
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<li>2.5 g NaCl</li>
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<li><a href="http://ecoliwiki.net/colipedia/index.php/DH5_alpha"><font size="3" color="#F0F8FF" face="Arial regular"><b>DH5&#x03B1; (homehold)</b></font></a></li>
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<li>8.0 g tryptone</li>
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<li><a href="http://ecoliwiki.net/colipedia/index.php/BL21%28DE3%29"><font size="3" color="#F0F8FF" face="Arial regular"><b>BL21DE3 (homehold)</b></font></a></li>
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<li>5.0 g yeast extract</li>
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<li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li>
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<br>
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LB (recipe for 1000 mL)
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<ul>
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<li>10 g tryptone</li>
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<li>5 g yeast extract</li>
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<li>10 g NaCl</li>
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<li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li>
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<li>for plates: add 15g agar</li>
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</ul>
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<br>
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SOB (recipe for 1000 mL)
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<ul>
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<li>0.186 g KCl</li>
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<li>2.4 g MgSO4</li>
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<li>0.584 g NaCl</li>
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<li>20 g tryptone</li>
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<li>5 g yeast extract</li>
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<li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li>
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</ul>
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<br>
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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SOC (recipe for 1000 mL)
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</p>
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<ul>
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<li>980 mL SOB</li>
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<li>20 mL 1M Glucose</li>
</ul>
</ul>
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Revision as of 19:33, 4 October 2013







Labbook | Materials | Protocols

Media

1.7-DYT (recipe for 500 mL)

  • 2.5 g NaCl
  • 8.0 g tryptone
  • 5.0 g yeast extract
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

LB (recipe for 1000 mL)

  • 10 g tryptone
  • 5 g yeast extract
  • 10 g NaCl
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
  • for plates: add 15g agar

SOB (recipe for 1000 mL)

  • 0.186 g KCl
  • 2.4 g MgSO4
  • 0.584 g NaCl
  • 20 g tryptone
  • 5 g yeast extract
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

SOC (recipe for 1000 mL)

  • 980 mL SOB
  • 20 mL 1M Glucose

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