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Team:TU Darmstadt/materials/Stock solutions
From 2013.igem.org
(Difference between revisions)
| Line 284: | Line 284: | ||
<p text-aligne:left style="margin-left:0px; margin-right:50px"> | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
Antibiotics | Antibiotics | ||
| - | </p></font | + | </p></font> |
<ul> | <ul> | ||
| + | <font size="4" color="#F0F8FF" face="Arial regular"> | ||
| + | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
Ampicillin | Ampicillin | ||
| + | </p></font> | ||
<ul> | <ul> | ||
1.Mix<br> | 1.Mix<br> | ||
| Line 298: | Line 301: | ||
</ul> | </ul> | ||
<br> | <br> | ||
| - | < | + | <font size="4" color="#F0F8FF" face="Arial regular"> |
| + | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
| + | Chloramphenicol | ||
| + | </p></font> | ||
<ul> | <ul> | ||
1.Mix<br> | 1.Mix<br> | ||
| Line 306: | Line 312: | ||
</ul> | </ul> | ||
2.aliquot in 1mL stocks and store at -20°C<br> | 2.aliquot in 1mL stocks and store at -20°C<br> | ||
| - | <br> | + | 3. use 1µL per 1mL medium<br><br> |
</ul> | </ul> | ||
<li>Kanamycin</li> | <li>Kanamycin</li> | ||
Revision as of 03:46, 5 October 2013
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Stock solutions
Antibiotics
Ampicillin
Chloramphenicol
1.Mix
2.sterile filtration
3. aliquot in 1 mL stocks and store at -20°C
4. use 1µL per 1mL medium
1.Mix
2.aliquot in 1mL stocks and store at -20°C
3. use 1µL per 1mL medium
1.Mix
2.sterile filtration
3.aliquot in 1 mL stocks and store at -20°C
IPTG Isopropyl-beta-D-thiogalactopyranoside
1. Dissolve 238 mg IPTG in 10 mL DW
2. store in 1mL aliquots at -20°C
tritrack loading dye
