Team:TU Darmstadt/protocols/DNA Quantification

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<h2><font size="6" color="#F0F8FF" face="Arial regular"> Bacterial cell culture </font></h2>
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<h2><font size="6" color="#F0F8FF" face="Arial regular"> DNA Quantification with NanoDrop </font></h2>
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4. Load your DNA samples and click "Measure"<br>
4. Load your DNA samples and click "Measure"<br>
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The system calculate automatically the 260/280 value and the 260/230 value.  
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The system calculate automatically the 260/280 value and the 260/230 value.
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<B>References<br>
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http://openwetware.org/wiki/Bacterial_cell_culture
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Revision as of 15:54, 4 October 2013





DNA Quantification with NanoDrop

Materials

Equipment
Micropipettes with sterile tips
Nanodrop

Chemicals & consumables
DNA probe
Water

Procedure
1. Start the programme for the NanoDrop and click on "Nucleic Acids"
2. Pipette 1 µL of a water sample onto the lower measurement and click “OK” for initialising
3. Load your blank and click "Blank"
4. Load your DNA samples and click "Measure"

The system calculate automatically the 260/280 value and the 260/230 value.