Team:TU Darmstadt/protocols/Heat Shock Transformation

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<h2><font size="6" color="#F0F8FF" face="Arial regular"> Heat Shock Transformation </font></h2>
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<B> Materials<br></B>
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Ice<br>
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Heatingbad<br>
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Pipets + steril tips<br>
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DYT-Media<br>
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LB-Agar-Plates + antibiotics<br>
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Incubator<br>
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<br>
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<B> Procedure<br></B>
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1. Defrost stocks of competent cells (100 µL in 1.5 ml Eppendorf tube) on ice<br>
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2. Add the DNA and incubate the suspension for 5 minutes on ice<br>
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3. Heat shock is done by incubating the cells for 1 minute at 42°C<br>
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4. Add 1 mL of DYT medium and incubate for 1 hour at 37°C in order to obtain antibiotic resistance<br>
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5. Finally the cells are ready to be crossed out<br>
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<br>

Revision as of 20:53, 3 October 2013

Labbook | Protocols | Materials |





Heat Shock Transformation

Materials
 Ice
Heatingbad
Pipets + steril tips
DYT-Media
LB-Agar-Plates + antibiotics
Incubator

Procedure
 1. Defrost stocks of competent cells (100 µL in 1.5 ml Eppendorf tube) on ice
2. Add the DNA and incubate the suspension for 5 minutes on ice
3. Heat shock is done by incubating the cells for 1 minute at 42°C
4. Add 1 mL of DYT medium and incubate for 1 hour at 37°C in order to obtain antibiotic resistance
5. Finally the cells are ready to be crossed out

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation"