Team:TU Darmstadt/protocols/PCR

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<h2><font size="6" color="#F0F8FF" face="Arial regular"> PCR </font></h2>
<h2><font size="6" color="#F0F8FF" face="Arial regular"> PCR </font></h2>
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Latest revision as of 00:37, 5 October 2013







Lab book | Materials | Protocols

PCR

Short report
 PCR (polymerase chain reaction) is a method for exponentially amplifying a fragment of DNA in vitro. Materials
Equipment
 Micropipettes with sterile tips
PCR machine
PCR tubes

Chemicals & consumables
DNA template
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO

Procedure
 This is a commonly used protocol thta we used for Taq-polymerase
PCR program (30 cycles)
 initial denaturation 95°C, 60s
denaturation 95°C, 45s
annealing 54,5°C, 90s
elongation 72°C, 120s
final elongation 72°C, 120s

After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega)

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