Team:TU Darmstadt/protocols/Restriction digest
From 2013.igem.org
Restriction digest
Short explanation
In order to insert DNA fragments into plasmids via ligation it is necessary to digest both components with restriction enzymes.
Procedure
DNA Digestion (50 µL batch)
Add up the following:
10x NEBuffer 5 µL
DNA 1 µg
Restriction Enzyme (10 u) 1 µL
100x BSA 0.2 µL
Add dH2O for total reaction volume of 50 µL
Incubate for 1 h at an temperatur of 37°C
inactivate enzymes by incubating at 65°C for 20 min