Team:Tianjin/Notebook

From 2013.igem.org

(Difference between revisions)
(June 26th, 2013)
(July, 22nd, 2013)
Line 690: Line 690:
[[File:Tju-notebook-1.6.jpg|center|330px|gene 8]]
[[File:Tju-notebook-1.6.jpg|center|330px|gene 8]]
-
<html>
 
 +
 +
[[File:Tju-gel-1.6.jpg|center|400px|gel 8]]
 +
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 8.</b>&nbsp;YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5</div>
<a name="anchor-n-07" id="anchor-n-07"></a>
<a name="anchor-n-07" id="anchor-n-07"></a>

Revision as of 16:42, 26 September 2013

  • June,2013
    • Sun Mon Tue Wed Thu Fri Sat
                  1
      2 3 4 5 6 7 8
      9 10 11 12 13 14 15
      16 17 18 19 20 21 22
      23 24 25 26 27 28 29
      30            
  • July,2013
    • Sun Mon Tue Wed Thu Fri Sat
        1 2 3 4 5 6
      7 8 9 10 11 12 13
      14 15 16 17 18 19 20
      21 22 23 24 25 26 27
      28 29 30 31      
  • August,2013
    • Sun Mon Tue Wed Thu Fri Sat
              1 2 3
      4 5 6 7 8 9 10
      11 12 13 14 15 16 17
      18 19 20 21 22 23 24
      25 26 27 28 29 30 31
  • September,2013
    • Sun Mon Tue Wed Thu Fri Sat
      1 2 3 4 5 6 7
      8 9 10 11 12 13 14
      15 16 17 18 19 20 21
      22 23 24 25 26 27 28
      29 30          

Contents

June 7th, 2013



Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.

gene


gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT


gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

June 20th, 2013



Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.


gene


gene


gene


gene


gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

June 26th, 2013



Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.

gene


gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

July, 5th, 2013



Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.

gene


gel 7

Figure7. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3

July 9th, 2013



Translated E. coli BL21 with plasmids constructed.


July, 22nd, 2013



Acquired YbhGFSR through PCR and linked it with pSB3C5.

gene 8


gel 8

Figure 8. YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5

August, 1st, 2013



Constructed J23100-RBS(B0030)-YbhGFSR-pSB1C3 (BBa_K081005).

gene


August, 10th, 2013



Constructed J23100-RBS(B0030)-YbhGFSR-pSB3C5.

gene 10


August 13th, 2013



Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.

gene


gene


gene



August, 18th, 2013



Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.

gene


gene


August 23rd, 2013



Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.

gene


gene

Retrieved from "http://2013.igem.org/Team:Tianjin/Notebook"