Team:Tianjin/Notebook

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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    <li><a href=" https://2013.igem.org/Team:Tianjin" style="padding:20px 0px 15px 0px;height:30px;">Home</a>
 +
</li>
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    <li><a href=" https://2013.igem.org/Team:Tianjin/Project" style="padding:20px 0px 15px 0px;height:30px;">Project</a>
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</li>
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    <li><a href=" https://2013.igem.org/Team:Tianjin/Safety "  style="padding:20px 0px 15px 0px;height:30px;">Safety</a>
 +
</li>
 +
    <li><a href=" https://2013.igem.org/Team:Tianjin/Modeling"  style="padding:20px 0px 15px 0px;height:30px;">Modeling</a>
 +
</li>
 +
    <li><a href=" https://2013.igem.org/Team:Tianjin/Protocol"  style="padding:20px 0px 15px 0px;height:30px;">Protocol</a>
 +
</li>
 +
    <li class="HP"><a href="https://2013.igem.org/Team:Tianjin/Human Practice" style="padding:14px 0px 13px 0px;">Human Practice</a>
 +
        </li>
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    <li><a href=" https://2013.igem.org/Team:Tianjin/Data"  style="padding:20px 0px 15px 0px;height:30px;">Data</a>
 +
</li>
 +
    <li><a href=" https://2013.igem.org/Team:Tianjin/Notebook"  style="padding:20px 0px 15px 0px;height:30px;">Notebook</a>
 +
</li>
 +
    <li><a href=" https://2013.igem.org/Team:Tianjin/Team"  style="padding:20px 0px 15px 0px;height:30px;">Team</a>
 +
</li>
 +
    <li class="A-C"><a href="https://2013.igem.org/Team:Tianjin/Attributions"  style="padding:7px 0px 7px 0px;">Attributions<br/>&amp;<br/>Contributions</a>
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        </li>
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        <ul>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/Team:Tianjin/Notebook">June,2013</a>
 +
                <ul>
 +
                    <li> 
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<table class="table1">
 +
<tr>
 +
<th>Sun</th>
 +
<th>Mon</th>
 +
<th>Tue</th>
 +
<th>Wed</th>
 +
<th>Thu</th>
 +
<th>Fri</th>
 +
<th>Sat</th>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>1</td>
 +
</tr>
 +
 +
<tr>
 +
<td>2</td>
 +
<td>3</td>
 +
<td>4</td>
 +
<td>5</td>
 +
<td>6</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-01">7</a></td>
 +
<td>8</td>
 +
</tr>
 +
 +
<tr>
 +
<td>9</td>
 +
<td>10</td>
 +
<td>11</td>
 +
<td>12</td>
 +
<td>13</td>
 +
<td>14</td>
 +
<td>15</td>
 +
</tr>
 +
 +
<tr>
 +
<td>16</td>
 +
<td>17</td>
 +
<td>18</td>
 +
<td>19</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-02">20</a></td>
 +
<td>21</td>
 +
<td>22</td>
 +
</tr>
 +
 +
<tr>
 +
<td>23</td>
 +
<td>24</td>
 +
<td>25</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-03">26</a></td>
 +
<td>27</td>
 +
<td>28</td>
 +
<td>29</td>
 +
</tr>
 +
 +
<tr>
 +
<td>30</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
</tr>
 +
 +
 +
</table>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/Team:Tianjin/Notebook">July,2013</a>
 +
                <ul>
 +
                    <li>
 +
             
 +
<table class="table1">
 +
<tr>
 +
<th>Sun</th>
 +
<th>Mon</th>
 +
<th>Tue</th>
 +
<th>Wed</th>
 +
<th>Thu</th>
 +
<th>Fri</th>
 +
<th>Sat</th>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td>1</td>
 +
<td>2</td>
 +
<td>3</td>
 +
<td>4</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-04">5</a></td>
 +
<td>6</td>
 +
</tr>
 +
 +
<tr>
 +
<td>7</td>
 +
<td>8</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-05">9</a></td>
 +
<td>10</td>
 +
<td>11</td>
 +
<td>12</td>
 +
<td>13</td>
 +
</tr>
 +
 +
<tr>
 +
<td>14</td>
 +
<td>15</td>
 +
<td>16</td>
 +
<td>17</td>
 +
<td>18</td>
 +
<td>19</td>
 +
<td>20</td>
 +
</tr>
 +
 +
<tr>
 +
<td>21</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-06">22</a></td>
 +
<td>23</td>
 +
<td>24</td>
 +
<td>25</td>
 +
<td>26</td>
 +
<td>27</td>
 +
</tr>
 +
 +
<tr>
 +
<td>28</td>
 +
<td>29</td>
 +
<td>30</td>
 +
<td>31</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
</tr>
 +
 +
 +
</table>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/Team:Tianjin/Notebook">August,2013</a>
 +
                <ul>
 +
                    <li>
 +
         
 +
<table class="table1">
 +
<tr>
 +
<th>Sun</th>
 +
<th>Mon</th>
 +
<th>Tue</th>
 +
<th>Wed</th>
 +
<th>Thu</th>
 +
<th>Fri</th>
 +
<th>Sat</th>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td>&nbsp;</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-07">1</a></td>
 +
<td>2</td>
 +
<td>3</td>
 +
</tr>
 +
 +
<tr>
 +
<td>4</td>
 +
<td>5</td>
 +
<td>6</td>
 +
<td>7</td>
 +
<td>8</td>
 +
<td>9</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-08">10</a></td>
 +
</tr>
 +
 +
<tr>
 +
<td>11</td>
 +
<td>12</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-09">13</a></td>
 +
<td>14</td>
 +
<td>15</td>
 +
<td>16</td>
 +
<td>17</td>
 +
</tr>
 +
 +
<tr>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-10">18</a></td>
 +
<td>19</td>
 +
<td>20</td>
 +
<td>21</td>
 +
<td>22</td>
 +
<td id="alt"><a href="https://2013.igem.org/Team:Tianjin/Notebook#anchor-n-03">23</a></td>
 +
<td>24</td>
 +
</tr>
 +
 +
<tr>
 +
<td>25</td>
 +
<td>26</td>
 +
<td>27</td>
 +
<td>28</td>
 +
<td>29</td>
 +
<td>30</td>
 +
<td>31</td>
 +
</tr>
 +
 +
</table>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li class="hmain">
 +
                <a href="https://2013.igem.org/wiki/index.php?title=Team:Tianjin/Notebook/HP">Human Practice Notebook</a>
 +
            </li>
 +
            <li>
 +
                <a href="https://2013.igem.org/Main_Page" title="Main Page"><img src="https://static.igem.org/mediawiki/2013/7/75/IGEM-logo-blue.png" width="200px" height="200px" border="none" style="margin-top:80px;"/></a>
 +
            </li>
 +
        </ul>
 +
</div>
 +
 +
</div>
 +
</div>
 +
 +
 +
<div class="main">
 +
<a name="anchor-n-01" id="anchor-n-01"></a>
</html>
</html>
-
<!-- *** End of the alert box *** -->
+
= June 7th, 2013=
 +
<html>
 +
<hr /><br />
 +
<p> Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.</p>
 +
</html>
 +
[[File:Tju-notebook-1.1.jpg|center|400px|gene]]
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
-
!align="center"|[[Team:Tianjin|Home]]
 
-
!align="center"|[[Team:Tianjin/Team|Team]]
 
-
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Tianjin Official Team Profile]
 
-
!align="center"|[[Team:Tianjin/Project|Project]]
 
-
!align="center"|[[Team:Tianjin/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:Tianjin/Modeling|Modeling]]
 
-
!align="center"|[[Team:Tianjin/Notebook|Notebook]]
 
-
!align="center"|[[Team:Tianjin/Safety|Safety]]
 
-
!align="center"|[[Team:Tianjin/Attributions|Attributions]]
 
-
|}
 
 +
[[File:Tju-gel-1.1.1.jpg|center|330px|gel 1]]
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 1.</b>&nbsp;AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT</div>
 +
</html>
-
If you choose to include a '''Modeling''' page, please write about your modeling adventures here. This is not necessary but it may be a nice list to include.
+
 
 +
[[File:Tju-gel-1.1.2.jpg|center|320px|gel 2]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 2.</b>&nbsp;AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT</div>
 +
</html>
 +
 
 +
 
 +
[[File:Tju-gel-1.1.3.jpg|center|330px|gel 3]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 3.</b>&nbsp;J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT</div>
 +
 
 +
<a name="anchor-n-02" id="anchor-n-02"></a>
 +
<br/>
 +
</html>
 +
 
 +
= June 20th, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.</p>
 +
 
 +
</html>
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-1.2.1.jpg|center|450px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-1.2.2.jpg|center|450px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-1.2.3.jpg|center|450px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-1.2.4.jpg|center|450px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-1.2.1.jpg|center|500px|gel 4]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 4.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3</div>
 +
</html>
 +
 
 +
 
 +
[[File:Tju-gel-1.2.2.jpg|center|460px|gel 5]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 5.</b>&nbsp;J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3</div>
 +
 
 +
 
 +
<a name="anchor-n-03" id="anchor-n-03"></a>
 +
<br/>
 +
</html>
 +
 
 +
= June 26th, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.</p>
 +
</html>
 +
 
 +
[[File:Tju-notebook-1.3.jpg|center|450px|gene]]
 +
 
 +
 
 +
[[File:Tju-gel-1.3.jpg|center|330px|gel 6]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 6.</b>&nbsp;J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3</div>
 +
 
 +
 
 +
<html>
 +
 
 +
<a name="anchor-n-04" id="anchor-n-04"></a>
 +
<br/>
 +
</html>
 +
 
 +
= July 5th, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.</p>
 +
</html>
 +
 
 +
[[File:Tju-notebook-1.4.jpg|center|330px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-1.4.jpg|center|440px|gel 7]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure7.</b>&nbsp;J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3</div>
 +
 
 +
<a name="anchor-n-05" id="anchor-n-05"></a>
 +
<br/>
 +
</html>
 +
 
 +
= July 9th, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Translated E. coli BL21 with plasmids constructed.</p>
 +
 
 +
<a name="anchor-n-06" id="anchor-n-06"></a>
 +
<br/>
 +
</html>
 +
 
 +
= July 22nd, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Acquired YbhGFSR through PCR and linked it with pSB3C5.</p>
 +
 
 +
</html>
 +
 
 +
[[File:Tju-notebook-1.6.jpg|center|330px|gene 8]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-1.6.jpg|center|350px|gel 8]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 8.</b>&nbsp;YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5</div>
 +
 
 +
<a name="anchor-n-07" id="anchor-n-07"></a>
 +
<br/>
 +
</html>
 +
 
 +
= August 1st, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.</p>
 +
 
 +
 
 +
</html>
 +
 
 +
[[File:Tju-notebook-1.7.jpg|center|300px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-1.7.jpg|center|360px|gel 9]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 9.</b>&nbsp;J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB1C3（BBa_K081005）</div>
 +
 
 +
 
 +
 
 +
<a name="anchor-n-08" id="anchor-n-08"></a>
 +
<br/>
 +
</html>
 +
 
 +
= August 10th, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.</p>
 +
 
 +
 
 +
</html>
 +
 
 +
[[File:Tju-notebook-1.8.jpg|center|300px|gene 10]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-1.8.jpg|center|350px|gel 10]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 10.</b>&nbsp;J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB3C5</div>
 +
 
 +
 
 +
 
 +
 
 +
<a name="anchor-n-09" id="anchor-n-09"></a>
 +
<br/>
 +
</html>
 +
 
 +
= August 13th, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p> Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.</p>
 +
 
 +
</html>
 +
 
 +
[[File:Tju-notebook-2.1.1.jpg|center|500px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-2.1.2.jpg|center|500px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-2.1.3.jpg|center|500px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-2.1.1.jpg|center|350px|gel 11]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 11.</b>&nbsp;J23100/J23103-AlkR-tt ~1200bp cut from J23100/J23103-AlkR-tt-pSB1C3</div>
 +
</html>
 +
 
 +
 
 +
[[File:Tju-gel-2.1.2.jpg|center|370px|gel 12]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 12.</b>&nbsp;PalkM-RFP-tt ~1000bp cut from PalkM-RFP-tt-pSB1C3</div>
 +
</html>
 +
 
 +
 
 +
[[File:Tju-gel-2.1.3.jpg|center|360px|gel 13]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 13.</b>&nbsp;PalkM-tetA-tt ~1500bp cut from PalkM-tetA-tt-pSB1C3</div>
 +
 
 +
 
 +
 
 +
 
 +
<a name="anchor-n-10" id="anchor-n-10"></a>
 +
<br/>
 +
</html>
 +
 
 +
= August 18th, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p>Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.</p>
 +
 
 +
 
 +
</html>
 +
 
 +
[[File:Tju-notebook-2.2.1.jpg|center|400px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-2.2.2.jpg|center|400px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-2.2.1.jpg|center|500px|gel 14]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 14.</b>&nbsp;PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB1C3</div>
 +
</html>
 +
 
 +
 
 +
[[File:Tju-gel-2.2.2.jpg|center|450px|gel 15]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 15.</b>&nbsp;PalkM-tetA -tt- J23100/J23103-ALKR-tt ~2800bp cut from PalkM-tetA-tt- J23100/J23103-ALKR-tt-pSB1C3</div>
 +
 
 +
 
 +
<a name="anchor-n-11" id="anchor-n-11"></a>
 +
<br/>
 +
</html>
 +
 
 +
= August 23rd, 2013=
 +
 
 +
<html>
 +
<hr /><br />
 +
<p>Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.</p>
 +
 
 +
 
 +
</html>
 +
 
 +
[[File:Tju-notebook-2.3.1.jpg|center|400px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-notebook-2.3.2.jpg|center|400px|gene]]
 +
 
 +
 
 +
 
 +
[[File:Tju-gel-2.3.jpg|center|350px|gel 16]]
 +
 
 +
<html>
 +
<div style="text-align:center;">
 +
<b>Figure 16.</b>&nbsp;PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB4C5</div>
 +
 
 +
 
 +
 
 +
</div>
 +
 
 +
 
 +
</div>
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
<div class="note2">
 +
 
 +
</div>
 +
 
 +
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 +
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<p><br />
 +
<br />
 +
E-mail：austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code：300072
 +
<br />Copyright 2013 © Tianjin University iGEM Team
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Latest revision as of 01:00, 28 September 2013

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  • August,2013
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  • Human Practice Notebook

Contents

June 7th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.

gene


gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT


gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

June 20th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.


gene


gene


gene


gene


gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

June 26th, 2013


Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.

gene


gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

July 5th, 2013


Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.

gene


gel 7

Figure7. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB1K3

July 9th, 2013


Translated E. coli BL21 with plasmids constructed.


July 22nd, 2013


Acquired YbhGFSR through PCR and linked it with pSB3C5.

gene 8


gel 8

Figure 8. YbhGFSR ~5000bp cut from YbhGFSR- pSB3C5

August 1st, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.

gene


gel 9

Figure 9. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB1C3（BBa_K081005）

August 10th, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.

gene 10


gel 10

Figure 10. J23100-RBS（B0030）-YbhGFSR ~5000bp cut from J23100-RBS（B0030）-YbhGFSR-pSB3C5

August 13th, 2013


Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.

gene


gene


gene


gel 11

Figure 11. J23100/J23103-AlkR-tt ~1200bp cut from J23100/J23103-AlkR-tt-pSB1C3


gel 12

Figure 12. PalkM-RFP-tt ~1000bp cut from PalkM-RFP-tt-pSB1C3


gel 13

Figure 13. PalkM-tetA-tt ~1500bp cut from PalkM-tetA-tt-pSB1C3

August 18th, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.

gene


gene


gel 14

Figure 14. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB1C3


gel 15

Figure 15. PalkM-tetA -tt- J23100/J23103-ALKR-tt ~2800bp cut from PalkM-tetA-tt- J23100/J23103-ALKR-tt-pSB1C3

August 23rd, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.

gene


gene


gel 16

Figure 16. PalkM-RFP -tt- J23100/J23103-ALKR-tt ~2300bp cut from PalkM-RFP -tt- J23100/J23103-ALKR-tt-pSB4C5

Retrieved from "http://2013.igem.org/Team:Tianjin/Notebook"