Team:Tianjin/Project/Experiment/Exo-Induce
From 2013.igem.org
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<p>We add alkanes outside the cell to test the function of Alk-Sensor:</p> | <p>We add alkanes outside the cell to test the function of Alk-Sensor:</p> | ||
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<p><b> Result:</b></p> | <p><b> Result:</b></p> | ||
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<p> No. 55 is not red at all. No. 54 with water added shows a bit of red. No. 54 with alkanes added as inducers is the reddest among three. Alk-Sensor can respond to exogenous alkanes. Besides, No. 54 with water added is less red than with alkanes added because our sensor has a weaker response to water than to exogenous alkanes.</p> | <p> No. 55 is not red at all. No. 54 with water added shows a bit of red. No. 54 with alkanes added as inducers is the reddest among three. Alk-Sensor can respond to exogenous alkanes. Besides, No. 54 with water added is less red than with alkanes added because our sensor has a weaker response to water than to exogenous alkanes.</p> | ||
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<p><b> Characterization:</b> The two strains are cultured in M9 medium for about 12 hours. Adjust the concentration of suspension culture until OD reaches 0.6, then take out 3mL and add 300μL Octane(C8),Decane(C10),Dodecane(C12), Tetradecane(C14), Hexadecane(C16) and Octadecyl(C18) respectively. After that, induce for 24 hours. Measure the ratio of fluorescent indensity (fluorescent indensity per OD) of cells, which is shown below:</p> | <p><b> Characterization:</b> The two strains are cultured in M9 medium for about 12 hours. Adjust the concentration of suspension culture until OD reaches 0.6, then take out 3mL and add 300μL Octane(C8),Decane(C10),Dodecane(C12), Tetradecane(C14), Hexadecane(C16) and Octadecyl(C18) respectively. After that, induce for 24 hours. Measure the ratio of fluorescent indensity (fluorescent indensity per OD) of cells, which is shown below:</p> | ||
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<p>C8</p> | <p>C8</p> | ||
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<p>C15</p> | <p>C15</p> | ||
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Revision as of 21:05, 27 September 2013