Team:Tianjin/Project/Experiment/Exo-Induce
From 2013.igem.org
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<p>C15</p> | <p>C15</p> | ||
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- | + | <P><b>Result:</b> With the increasing concentration of alkanes, ratio of fluorescent indensity is also increased. There is a positive correlation between concentration and ratio. Using logarithmic fitting curve, we find that with the increasing concentration of alkanes, the increase of ratio of fluorescent indensity becomes smooth, approaching its upper limit at the concentration of 10μmol/L C8 in LB medium, and approaching its upper limit at the concentration of 1μmol/L C15 in LB medium. | |
+ | </P> | ||
<p> We choose the strains of No.54 and culture them in 3mL M9 medium until OD reaches 0.6,add C8 and C15 of a concentration range from100nmol/L to 1mmol/L, over-night cultured. All of them are utilized to measure fluorescence ntensity of cells.</p> | <p> We choose the strains of No.54 and culture them in 3mL M9 medium until OD reaches 0.6,add C8 and C15 of a concentration range from100nmol/L to 1mmol/L, over-night cultured. All of them are utilized to measure fluorescence ntensity of cells.</p> | ||
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<div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/a/a7/TJU-EX7.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/a/a7/TJU-EX7.jpg" width="700px" /></a></div> | <div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/a/a7/TJU-EX7.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/a/a7/TJU-EX7.jpg" width="700px" /></a></div> | ||
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- | + | <p><b>Result:</b> With the increasing concentration of alkanes, ratio of fluorescent indensity is also increased. There is a positive correlation between concentration and ratio. Using logarithmic fitting curve, we find that with the increasing concentration of alkanes, ratio of fluorescent indensity increases constantly. At the concentration of 10nmol/L to 2nmol/L of C8 and C15, stronger positive correlation is showed than higher concentration of C8 and C15. | |
+ | </p> | ||
<p> We choose the strains of No.54 and culture them in 3mL M9 medium, until OD reaches 0.6,add C8 and C15 of a concentration range from100nmol/L to 1mmol/L and 30uL DMSO, over-night cultured. All of them are utilized to measure fluorescence intensity of cells.</p> | <p> We choose the strains of No.54 and culture them in 3mL M9 medium, until OD reaches 0.6,add C8 and C15 of a concentration range from100nmol/L to 1mmol/L and 30uL DMSO, over-night cultured. All of them are utilized to measure fluorescence intensity of cells.</p> | ||
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<div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" width="700px" /></a></div> | <div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" width="700px" /></a></div> | ||
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+ | <p>Result: With the increasing concentration of alkanes, ratio of fluorescent indensity is also increased. There is a positive correlation between concentration and ratio. Using logarithmic fitting curve, we find that with the increasing concentration of alkanes, the increase of ratio of fluorescent indensity becomes smooth, approaching its upper limit at the concentration of 10μmol/L C8 in M9 medium, and approaching its upper limit at the concentration of 1μmol/L C15 in M9 medium. At the concentration of 10nmol/L to 2nmol/L of C8 and C15, strong positive correlation is also showed. </p> | ||
+ | <p>DMSO is a polar aprotic solvent which increases permeability of cell membranes and helps alkanes to induce alkR. However, compared with DMSO-free medium, growth of cells are inhibited.</p> | ||
Revision as of 21:09, 27 September 2013