Team:Tianjin/Project/Experiment/Exo-Induce
From 2013.igem.org
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<p> No. 55 is not red at all. No. 54 with water added shows a bit of red. No. 54 with alkanes added as inducers is the reddest among three. Alk-Sensor can respond to exogenous alkanes. Besides, No. 54 with water added is less red than with alkanes added because our sensor has a weaker response to water than to exogenous alkanes.</p> | <p> No. 55 is not red at all. No. 54 with water added shows a bit of red. No. 54 with alkanes added as inducers is the reddest among three. Alk-Sensor can respond to exogenous alkanes. Besides, No. 54 with water added is less red than with alkanes added because our sensor has a weaker response to water than to exogenous alkanes.</p> | ||
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<h2>Specific detection </h2> | <h2>Specific detection </h2> | ||
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<p>1.Alkanes are hydroforbic molecules, which makes alkanes with carbon chain lengths equal to or larger than 10 hard to dissolve in water to induce alkR. </p> | <p>1.Alkanes are hydroforbic molecules, which makes alkanes with carbon chain lengths equal to or larger than 10 hard to dissolve in water to induce alkR. </p> | ||
<p>2.Ratio of fluorescent indensity of Dodecane(C12) induced is larger than those induced by Decane(C10), proving that alkR has a specifical detection of Dodecane(C12). This is also consistent with the reference.</p> | <p>2.Ratio of fluorescent indensity of Dodecane(C12) induced is larger than those induced by Decane(C10), proving that alkR has a specifical detection of Dodecane(C12). This is also consistent with the reference.</p> | ||
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<h2>Upper limits of detection</h2> | <h2>Upper limits of detection</h2> | ||
<p>We choose Octane(C8), because it is commonly used to characterize alkane-sensor in most references, and we choose Pentadecane(C15), because our endogenous alkane producing module produce this kind of alkane. </p> | <p>We choose Octane(C8), because it is commonly used to characterize alkane-sensor in most references, and we choose Pentadecane(C15), because our endogenous alkane producing module produce this kind of alkane. </p> | ||
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<div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" width="700px" /></a></div> | <div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/2/25/TJU-EX9.jpg" width="700px" /></a></div> | ||
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- | <p>Result: With the increasing concentration of alkanes, ratio of fluorescent indensity is also increased. There is a positive correlation between concentration and ratio. Using logarithmic fitting curve, we find that with the increasing concentration of alkanes, the increase of ratio of fluorescent indensity becomes smooth, approaching its upper limit at the concentration of 10μmol/L C8 in M9 medium, and approaching its upper limit at the concentration of 1μmol/L C15 in M9 medium. At the concentration of 10nmol/L to 2nmol/L of C8 and C15, strong positive correlation is also showed. </p> | + | <p><b>Result:</b> With the increasing concentration of alkanes, ratio of fluorescent indensity is also increased. There is a positive correlation between concentration and ratio. Using logarithmic fitting curve, we find that with the increasing concentration of alkanes, the increase of ratio of fluorescent indensity becomes smooth, approaching its upper limit at the concentration of 10μmol/L C8 in M9 medium, and approaching its upper limit at the concentration of 1μmol/L C15 in M9 medium. At the concentration of 10nmol/L to 2nmol/L of C8 and C15, strong positive correlation is also showed. </p> |
<p>DMSO is a polar aprotic solvent which increases permeability of cell membranes and helps alkanes to induce alkR. However, compared with DMSO-free medium, growth of cells are inhibited.</p> | <p>DMSO is a polar aprotic solvent which increases permeability of cell membranes and helps alkanes to induce alkR. However, compared with DMSO-free medium, growth of cells are inhibited.</p> | ||
Revision as of 21:10, 27 September 2013