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Team:Tsinghua-E/Result
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| - | By using <a href="/Team:Tsinghua-E/Parts">the parts</a> constructed by us, following <a href="/Team:Tsinghua-E/Protocol">the protocol</a> described, we conducted the continuous evolution derived by mutD based mutation for two E. Coli BL21 (DE3) strains carrying bitter defender part 30 and 32. The results were shown below: | + | By using <html><a href="/Team:Tsinghua-E/Parts">the parts</a> constructed by us, following <a href="/Team:Tsinghua-E/Protocol">the protocol</a></html> described, we conducted the continuous evolution derived by mutD based mutation for two E. Coli BL21 (DE3) strains carrying bitter defender part 30 and 32. The results were shown below: |
Revision as of 02:32, 28 September 2013
Strains
BL21(DE3)(mut-bitter30(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA
BL21(DE3)(mut-bitter32(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA
Negative control：
BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA
BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA
Each evolution was conducted in three parallel with only one parallel of negative control.
Results
By using the parts constructed by us, following the protocol described, we conducted the continuous evolution derived by mutD based mutation for two E. Coli BL21 (DE3) strains carrying bitter defender part 30 and 32. The results were shown below:
