Team:Tsinghua-E/Result

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Strains

BL21(DE3)(mut-bitter30(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA

BL21(DE3)(mut-bitter32(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA

Negative control：

BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA

BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA

Each evolution was conducted in three parallel with only one parallel of negative control.

Results

By using <a href="/Team:Tsinghua-E/Parts">the parts</a> constructed by us, following <a href="/Team:Tsinghua-E/Protocol">the protocol</a> described, we conducted the continuous evolution derived by mutD based mutation for two E. Coli BL21 (DE3) strains carrying bitter defender part 30 and 32. The results were shown below:


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