BL21(DE3)(mut-bitter30(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA
BL21(DE3)(mut-bitter32(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA
Negative control:
BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA
BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA
Each evolution was conducted in three parallel with only one parallel of negative control.