Team:Tuebingen/Notebook/Protocols/pcr

From 2013.igem.org

Revision as of 00:51, 4 October 2013 by SvenB (Talk | contribs)

Return to iGEM Main Page.

PCR
Back to Protocols

 

Reagents

39.5 µL Aqua dest.
2.5 µL dNTPs (200 µM each)
1 µL DNA (c = 100 ng/µL)
5.0 µL Taq-Buffer
0.5 µL Forward primer
0.5 µL Reverse primer
1 µL Taq/Pfu polymerases (9+1)

 

Procedure

Mix all reagents in the given order (Important: add polymerases last!) and transfer in one 0.2 mL Eppendorf-tube ("PCR-tube")

This protocol yields exactly one single reaction mixture! Scale linearly for multiple reaction mixtures and keep mixture on ice or even better at -20 °C for best results.