"
Team:Tuebingen/Notebook/Protocols/sob-medium
From 2013.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
<head> | <head> | ||
<style> | <style> | ||
| - | + | h3 { | |
| + | margin-bottom: 20px; | ||
| + | } | ||
</style> | </style> | ||
</head> | </head> | ||
| Line 48: | Line 50: | ||
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. | + | <p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving.After autoclaving add 5 mL MgCL2 (c = 2 M).</p> |
| - | + | ||
</div> | </div> | ||
Revision as of 21:58, 3 October 2013
SOB Medium
Back to Protocols
Reagents
| 20.0 g | Trypton |
| 5.0 g | Yeast extract |
| 0.5 g | NaCl |
| 10 mL | 250 mM KCl |
Procedure
Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving.After autoclaving add 5 mL MgCL2 (c = 2 M).
