"
Team:Tuebingen/Notebook/Protocols/sob-medium
From 2013.igem.org
(Difference between revisions)
| Line 29: | Line 29: | ||
<tr> | <tr> | ||
<td style="text-align: center">20.0 g</td> | <td style="text-align: center">20.0 g</td> | ||
| - | <td> | + | <td>Trypton</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td style="text-align: center">5.0 g</td> | <td style="text-align: center">5.0 g</td> | ||
| - | <td> | + | <td>Yeast extract</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td style="text-align: center">0.5 g</td> | <td style="text-align: center">0.5 g</td> | ||
| - | <td> | + | <td>NaCl</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td style="text-align: center">10 mL</td> | <td style="text-align: center">10 mL</td> | ||
| - | <td> | + | <td>KCl (c = 250 mM)</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 22:01, 3 October 2013
SOB Medium
Back to Protocols
Reagents
| 20.0 g | Trypton |
| 5.0 g | Yeast extract |
| 0.5 g | NaCl |
| 10 mL | KCl (c = 250 mM) |
Procedure
Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).
