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Team:Tuebingen/Notebook/Protocols/sob-medium
From 2013.igem.org
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p> | + | <p>In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).</p> |
</div> | </div> | ||
Revision as of 03:09, 4 October 2013
SOB Medium
Back to Protocols
Reagents
| 20.0 g | Trypton |
| 5.0 g | Yeast extract |
| 0.5 g | NaCl |
| 10 mL | KCl (c = 250 mM) |
Procedure
In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).
