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Team:Tuebingen/Notebook/Protocols/sob-medium
From 2013.igem.org
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<h3>Reagents</h3> | <h3>Reagents</h3> | ||
<table border="0"> | <table border="0"> | ||
<colgroup> | <colgroup> | ||
| - | <col width=" | + | <col width="80"> |
<col width="300"> | <col width="300"> | ||
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<tr> | <tr> | ||
| - | <td style="text-align: | + | <td style="text-align: center">20.0 g</td> |
<td>Trypton</td> | <td>Trypton</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>5.0 g</td> | + | <td style="text-align: center">5.0 g</td> |
<td>Yeast extract</td> | <td>Yeast extract</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>0.5 g</td> | + | <td style="text-align: center">0.5 g</td> |
<td>NaCl</td> | <td>NaCl</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>10 mL</td> | + | <td style="text-align: center">10 mL</td> |
| - | <td>250 mM | + | <td>KCl (c = 250 mM)</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | <p> </p> | ||
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| + | <h3>Procedure</h3> | ||
| + | <p>In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).</p> | ||
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| + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
</div> | </div> | ||
Latest revision as of 12:08, 4 October 2013
SOB Medium
Reagents
| 20.0 g | Trypton |
| 5.0 g | Yeast extract |
| 0.5 g | NaCl |
| 10 mL | KCl (c = 250 mM) |
Procedure
In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).
Back to Protocols
