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Team:Tuebingen/Notebook/Protocols/ypd
From 2013.igem.org
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p>Mix all reagents in an autoclavable flask and fill up to 1000 mL with Aqua dest. Autoclave, let cool down a bit, and add antibiotics if required. Perform next step at laminar flow cabinet! Cast plates in petri dishes (approx. 20 mL per plate) | + | <p>Mix all reagents in an autoclavable flask and fill up to 1000 mL with Aqua dest. Autoclave, let cool down a bit, and add antibiotics if required. Perform next step at laminar flow cabinet! Cast plates in petri dishes (approx. 20 mL per plate). Store plates at 4 °C.</p> |
</div> | </div> | ||
Revision as of 03:07, 4 October 2013
YPD Medium
Back to Protocols
Reagents
| 10.0 g | >Yeast extract |
| 20.0 g | Peptone |
| 92.0 mg | Adenine-sulfate |
| 33 ng | Adenine |
| 16.6 g | Agar |
| to 1000 mL | Aqua dest. |
Procedure
Mix all reagents in an autoclavable flask and fill up to 1000 mL with Aqua dest. Autoclave, let cool down a bit, and add antibiotics if required. Perform next step at laminar flow cabinet! Cast plates in petri dishes (approx. 20 mL per plate). Store plates at 4 °C.
