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Team:Tuebingen/Notebook/Protocols/ypd
From 2013.igem.org
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If agar was added: cast plates in petri dishes (approx. 20 mL per plate) while medium is still warm. Store plates at 4 °C.</p> | If agar was added: cast plates in petri dishes (approx. 20 mL per plate) while medium is still warm. Store plates at 4 °C.</p> | ||
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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
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Latest revision as of 12:07, 4 October 2013
YPD Medium
Reagents
| 10.0 g | Yeast extract |
| 20.0 g | Peptone |
| 92.0 mg | Adenine-sulfate |
| 33 ng | Adenine |
| (16.6 g | Agar) |
| 50.0 mL | Sterile glucose (40 %) |
| to 1000 mL | Aqua dest. |
Procedure
For plates add agar, for liquid medium leave out agar.
Mix all reagents in an autoclavable flask and fill up to 1000 mL with Aqua dest. Autoclave, let cool down a bit, and add antibiotics if medium is required for selective purposes. Perform next steps at laminar flow cabinet! Add glucose solution. Store medium at 4 °C.
If agar was added: cast plates in petri dishes (approx. 20 mL per plate) while medium is still warm. Store plates at 4 °C.
