Team:UCL/Labbook

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<p class="major_title">Lab Weeks</p>
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<p class="major_title">June</p>
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<p class="minor_title">Week 1-3</p>
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<p class="body_text"><a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a>
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No lab work
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<p class="minor_title">Week 4</p>
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26th June - We are given safety training in the Advanced Centre for Biochemical Engineering in all relevant laboratories, as well as general procedures in case of emergency.
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Click the links above to view the corresponding Lab diary weeks
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<p class="major_title">July</p>
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<p class="minor_title">Week 5-6</p>
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No lab work
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<p class="minor_title">Week 7</p>
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Bacterial Lab
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15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.
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16th July - 5X M9 salts [link to protocol], minimal agar [link to protocol], 1.4% molten agar solution [link to protocol] and 0.1M CaCl2/15% glycerol [link to protocol] were prepared for the generation of competent cells. Minimal agar plates were poured and streaked [link to streaking protocol] with W3110 Escherichia coli cells and left overnight to incubate at 37C.
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17th July - Very little colony growth was observed from W3110 E.coli streaked plates. Plates were therefore left to incubate for a further 17 hours.
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18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media [link to LB recipe] + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.
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19th July - Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.
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Mammalian Lab
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</p>
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17th July - Mammalian cell culture and maintenance [link to mammalian protocol] training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
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</p>
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18th July - MEF passage 4
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</p>
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19th July - MEF passage 5
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</p>
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<p class="minor_title">Week 8</p>
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Bacterial Lab
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</p>
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22nd July -Transformation [link to transformation protocol] of our competent cells with plasmid YB3110 was carried out.
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</p>
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23rd July - No colony growth was observed on Ampicillin plates [link to ampicillin plate protocol] indicating no plasmid uptake. Transformation was repeated with YB3110.
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</p>
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<table>
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<tr>
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<th>Vial</th>
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<th>Ampicillin Plate</th>
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<th>Plasmid Insertion</th>
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<th>Colony Count</th>
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<td>1 (Main)</td>
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<td>Yes</td>
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<td>Yes</td>
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<td>0</td>
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<td>2 (Positive Control)</td>
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<td>No</td>
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<td>Yes</td>
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<td>100+</td>
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<td>3 (Negative Control)</td>
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<td>Yes</td>
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<td>No</td>
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<td>0</td>
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<p class="minor_title">Week 9</p>
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<p class="body_text">
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Bacterial Lab
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<p class="major_title">August</p>
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Latest revision as of 00:25, 5 October 2013

Lab Weeks

Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18

Click the links above to view the corresponding Lab diary weeks

Retrieved from "http://2013.igem.org/Team:UCL/Labbook"