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Team:UCL/Labbook/Week7
From 2013.igem.org
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19th July - Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C. | 19th July - Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C. | ||
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Revision as of 10:38, 3 October 2013
Lab Weeks
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20
July
Week 7
Bacterial Lab
15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.
16th July - 5X M9 salts, minimal agar, 1.4% molten agar solution and 0.1M CaCl2/15% glycerol were prepared for the generation of competent cells. Minimal agar plates were poured and streaked with W3110 Escherichia coli cells and left overnight to incubate at 37C.
17th July - Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.
18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.
19th July - Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.
Mammalian Lab
17th July - Mammalian cell culture and maintenance training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
18th July - MEF passage 4
19th July - MEF passage 5
