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| - | {{Team:HokkaidoU_Japan/Index/Slideshow}}
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| - | {{Team:HokkaidoU_Japan/header}}
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| - | <html>
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| - | <div id="hokkaidou-left-content">
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| - | <ul id="hokkaidou-index-menu">
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| - | <li id="hokkaidou-index-team"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Team">Team
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| - | <span class="hokkaidou-description">
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| - | The HokkaidoU_Japan is formed by 16 students and 3 instructors
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| - | </span>
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| - | </a>
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| - | </li>
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| - | <li id="hokkaidou-index-project"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Project">Project
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| - | <span class="hokkaidou-description">
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| - | We tried to further develop E. coli-based protein injection system which we started working on during iGEM 2010
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| - | </span>
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| - | </a>
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| - | </li>
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| - | <li id="hokkaidou-index-parts"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Parts">Parts
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| - | <span class="hokkaidou-description">
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| - | Ready-to-inject backbone and Bsa I cloning system
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| - | </span>
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| - | </a>
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| - | </li>
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| - | <li id="hokkaidou-index-protocol"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Protocols">Protocol
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| - | <span class="hokkaidou-description">
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| - | General protocols, Primers and methods of infection assay
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| - | </span>
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| - | </a>
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| - | </li>
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| - | <li id="hokkaidou-index-notebook"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Notebook">Notebook
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| - | <span class="hokkaidou-description">
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| - | The record of our many failures and a bit of achievement
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| - | </span>
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| - | </a>
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| - | </li>
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| - | <li id="hokkaidou-index-safety"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Safety">Safety
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| - | <span class="hokkaidou-description">
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| - | Addressing safety concerns
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| - | </span>
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| - | </a>
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| - | </li>
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| - | <li id="hokkaidou-index-humanpractice"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice
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| - | <span class="hokkaidou-description">
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| - | Science art gallery "BIO ART"
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| - | </span>
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| - | </a>
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| - | </li>
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| - | </ul>
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| - | <div id="hokkaidou-official-team-logo">
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| - | <img src="http://igemhokkaidou.files.wordpress.com/2011/04/igem-hokkaidou-logo-transparent.png?w=240&h=240#038;h=240" width="240" height="240" />
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| - | <span class="hokkaidou-description">iGEM HokkaidoU_Japan Official Team Logo</span>
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| - | </div>
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| - | <div id="hokkaidou-links">
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| - | <ul>
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| - | <li id="hokkaidou-links-email"><a href="mailto:igemhokkaidou@gmail.com">Email</a></li>
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| - | <li id="hokkaidou-links-facebook"><a href="http://www.facebook.com/iGEMHokkaidoU">Facebook</a></li>
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| - | <li id="hokkaidou-links-twitter"><a href="http://twitter.com/iGEM_HokkaidoU">Twitter</a></li>
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| - | <li id="hokkaidou-links-wordpress"><a href="http://igemhokkaidou.com">WordPress</a></li>
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| - | <li id="hokkaidou-links-youtube"><a href="http://www.youtube.com/user/iGEMHokkaidoU">YouTube</a></li>
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| - | </ul>
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| - | </div>
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| - | </html>
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| - | {{Team:HokkaidoU_Japan/SocialButtons}}
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| - | <div id="hokkaidou-right-content">
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| - | <div id="lofslidecontent45" class="lof-slidecontent">
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| - | <div style="display: none;" class="preload"><div></div></div>
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| - | <div class="lof-main-outer" style="width:900px; height:340px;">
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| - | <ul style="left: -4200px; width: 6300px;" class="lof-main-wapper">
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| - | <li>
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| - | <img src="https://static.igem.org/mediawiki/2011/6/64/IGEM_HokkaidoU_Index_Slideshow_7.jpg">
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| - | <div class="lof-main-item-desc">
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| - | <h2>HokkaidoU_Japan 2011</h2>
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| - | </div>
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| - | </li>
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| - | <li>
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| - | <img src="https://static.igem.org/mediawiki/2011/8/8a/IGEM_HokkaidoU_Index_Slideshow_1.jpg">
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| - | <div class="lof-main-item-desc">
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| - | <h2>Welcome to the BIOxART Gallery!!</h2>
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| - | </div>
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| - | </li>
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| - | <li>
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| - | <img src="https://static.igem.org/mediawiki/2011/b/b5/IGEM_HokkaidoU_Index_Slideshow_2.jpg">
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| - | <div class="lof-main-item-desc">
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| - | <h2>Be careful not to contaminate...</h2>
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| - | </div>
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| - | </li>
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| - | <li>
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| - | <img src="https://static.igem.org/mediawiki/2011/0/01/IGEM_HokkaidoU_Index_Slideshow_3.jpg">
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| - | <div class="lof-main-item-desc">
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| - | <h2>Meeting, meeting and MEETING!</h2>
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| - | </div>
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| - | </li>
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| - | <li>
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| - | <img src="https://static.igem.org/mediawiki/2011/d/d1/IGEM_HokkaidoU_Index_Slideshow_4.jpg">
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| - | <div class="lof-main-item-desc">
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| - | <h2>Dr. <i>E. coli</i> get ready for the job</h2>
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| - | </div>
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| - | </li>
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| - | <li>
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| - | <img src="https://static.igem.org/mediawiki/2011/0/06/IGEM_HokkaidoU_Index_Slideshow_5.jpg">
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| - | <div class="lof-main-item-desc">
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| - | <h2>BIOxART, please feel free to look around</h2>
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| - | </div>
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| - | </li>
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| - | <li>
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| - | <img src="https://static.igem.org/mediawiki/2011/7/74/IGEM_HokkaidoU_Index_Slideshow_6.jpg">
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| - | <h2>Experiment, experiment and EXPERIMENT!</h2>
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| - | </div>
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| - | </li>
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| - | </ul>
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| - | </div>
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| - | <div class="lof-navigator-wapper">
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| - | <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/f3/IGEM_HokkaidoU_Index_Slideshow_Thumb_1.jpg"></li>
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| - | <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/a/aa/IGEM_HokkaidoU_Index_Slideshow_Thumb_2.jpg"></li>
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| - | <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/fb/IGEM_HokkaidoU_Index_Slideshow_Thumb_3.jpg"></li>
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| - | <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/e/e2/IGEM_HokkaidoU_Index_Slideshow_Thumb_4.jpg"></li>
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| - | <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/9/93/IGEM_HokkaidoU_Index_Slideshow_Thumb_5.jpg"></li>
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| - | <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/2/21/IGEM_HokkaidoU_Index_Slideshow_Thumb_6.jpg"></li>
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| - | </ul>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <h2>Abstract</h2>
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| - | <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
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| - | <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
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| - | <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
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| - | <h2>BioArt</h2>
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| - | <iframe id="hokkaidou-bioart-youtube" width="680" height="360" src="http://www.youtube.com/embed/nOP0YIUSj-s" frameborder="0" allowfullscreen></iframe>
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| - | <style type="text/css">
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| - | iframe#hokkaidou-bioart-youtube{ margin-bottom: 30px; }
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| - | </style>
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| - | <p>We held the new type science art gallery "Bio Art" at the center of Sapporo city.</p>
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| - | <p>This gallery is not only for our team.You can also enjoy Bio Art on this Web page.
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| - | You can watch more detail in <a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice</a> page.</p>
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| - | </html>
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| - |
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| - | ==Acknowledgements==
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| - | ===Laboratory and Personnel===
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| - | * Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
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| - | :: Ken-ichi Yamazaki and Members of Yamazaki Lab
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| - | * Molecular Biology, Institute for Genetic Medicine, Hokkaido University
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| - | :: Hisatoshi Shida
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| - | * Nikon Imaging Center
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| - | :: Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino
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| - | * Laboratory of Molecular and Cellular Biophysics, Hokkaido University
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| - | :: Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami
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| - | * Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University
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| - | :: Taizo Motomura
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| - | * Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science
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| - | ::Isao Tanaka
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| - | * Research Institute for Electronic Science, Hokkaido University
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| - |
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| - | ===Academic Institutions===
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| - | * Salmonella Genetic Stock Center/University of Calgary
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| - | * Graduate School of Environmental Earth Science
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| - | * Department of biological science/School of Sciense
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| - | * Institute for Genetic Medicine
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| - |
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| - | ===Companies===
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| - | * Primary Cell Co., Ltd.
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| - | * ECONiXE Co., Ltd.
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| - | * Hokuto Kohki Engineering Co,. Ltd.
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| - | * COSMO BIO Co., Ltd.
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| - | * AMINO UP CHEMICAL Co., Ltd.
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| - | * Mendel Workshop Co., Ltd.
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| - |
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| - | </div>
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| - | {{Team:HokkaidoU_Japan/footer}}
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