Team:UCSF/Home1

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/*! Copyright (c) 2009 Brandon Aaron (http://brandonaaron.net)
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* Dual licensed under the MIT (http://www.opensource.org/licenses/mit-license.php)
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<div class="row main-image">
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* and GPL (http://www.opensource.org/licenses/gpl-license.php) licenses.
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<div class="twelve columns no-pad" style="margin-bottom:20px;">
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* Thanks to: http://adomas.org/javascript-mouse-wheel/ for some pointers.
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<div id="homepage-slideshow">
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* Thanks to: Mathias Bank(http://www.mathias-bank.de) for a scope bug fix.
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<img src="https://static.igem.org/mediawiki/2012/1/19/Cornell1Sliders.jpg" />
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*
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<div class="row">
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(function($) {
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<div class="six columns homepage-section tl">
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<div class="row">
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<div class="five columns homepage-thumb">
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<a href="https://2012.igem.org/Team:Cornell/project"><img src="https://static.igem.org/mediawiki/2012/1/13/Cornell_Dan_SQ.jpg"></a>
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<div class="row">
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<div class="twelve columns">
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<a href="https://2012.igem.org/Team:Cornell/project"><h3 style="margin:0px auto 0px;">Project</h3></a>
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</div>
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</div>
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<div class="row">
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<div class="twelve columns">
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Discover our novel approach to environmental biosensing, where synthetic biology intersects with microbial electrochemical systems.
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var types = ['DOMMouseScroll', 'mousewheel'];
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</div>
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<div class="row">
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<div class="twelve columns">
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See how our wetlab and drylab subteams operated on a day-to-day basis in order to accomplish our goals of constructing a field-deployable biosensor with direct electric output.
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$.event.special.mousewheel = {
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Meet our dedicated team of undergraduates who conceived of, planned, and carried out all the biological, mechanical and electrical engineering to complete our device.
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Examine our efforts to reach out to our local community &#8212; involving presentations to other scientists, discussions with fellow students and interaction with educators and young children.
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</div>
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</div>
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<div class="row">
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* @website    http://landofcoder.com
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this.navigatorInner = this.navigatorOuter.find( this.settings.navInnerSelector );
 +
 +
if( this.settings.navPosition == 'horizontal' ){
 +
this.navigatorInner.width( this.slides.length * this.settings.navigatorWidth );
 +
this.navigatorOuter.width( this.settings.maxItemDisplay * this.settings.navigatorWidth );
 +
this.navigatorOuter.height( this.settings.navigatorHeight );
 +
 +
} else {
 +
this.navigatorInner.height( this.slides.length * this.settings.navigatorHeight );
 +
 +
this.navigatorOuter.height( this.settings.maxItemDisplay * this.settings.navigatorHeight );
 +
this.navigatorOuter.width( this.settings.navigatorWidth );
 +
}
 +
this.navigratorStep = this.__getPositionMode( this.settings.navPosition );
 +
this.directionMode = this.__getDirectionMode(); 
 +
 +
 +
if( this.settings.direction == 'opacity') {
 +
this.wrapper.addClass( 'lof-opacity' );
 +
$(this.slides).css('opacity',0).eq(this.currentNo).css('opacity',1);
 +
} else {
 +
this.wrapper.css({'left':'-'+this.currentNo*this.maxSize+'px', 'width':( this.maxWidth ) * this.slides.length } );
 +
}
 +
 
 +
 +
if( this.settings.isPreloaded ) {
 +
this.preLoadImage( this.onComplete );
 +
} else {
 +
this.onComplete();
 +
}
 +
 +
}
 +
    $.lofSidernews.fn =  $.lofSidernews.prototype;
 +
    $.lofSidernews.fn.extend =  $.lofSidernews.extend = $.extend;
 +
 +
$.lofSidernews.fn.extend({
 +
 
 +
startUp:function( obj, wrapper ) {
 +
seft = this;
 +
 
 +
this.navigatorItems.each( function(index, item ){
 +
$(item).click( function(){
 +
seft.jumping( index, true );
 +
seft.setNavActive( index, item );
 +
} );
 +
$(item).css( {'height': seft.settings.navigatorHeight, 'width':  seft.settings.navigatorWidth} );
 +
})
 +
this.registerWheelHandler( this.navigatorOuter, this );
 +
this.setNavActive(this.currentNo );
 +
 +
if( this.settings.buttons && typeof (this.settings.buttons) == "object" ){
 +
this.registerButtonsControl( 'click', this.settings.buttons, this );
 +
 
 +
}
 +
if( this.settings.auto )
 +
this.play( this.settings.interval,'next', true );
 +
 +
return this;
 +
},
 +
onComplete:function(){
 +
setTimeout( function(){ $('.preload').fadeOut( 900 ); }, 400 ); this.startUp( );
 +
},
 +
preLoadImage:function(  callback ){
 +
var self = this;
 +
var images = this.wrapper.find( 'img' );
 +
 +
var count = 0;
 +
images.each( function(index,image){
 +
if( !image.complete ){  
 +
image.onload =function(){
 +
count++;
 +
if( count >= images.length ){
 +
self.onComplete();
 +
}
 +
}
 +
image.onerror =function(){
 +
count++;
 +
if( count >= images.length ){
 +
self.onComplete();
 +
}
 +
}
 +
}else {
 +
count++;
 +
if( count >= images.length ){
 +
self.onComplete();
 +
}
 +
}
 +
} );
 +
},
 +
navivationAnimate:function( currentIndex ) {
 +
if (currentIndex <= this.settings.startItem
 +
|| currentIndex - this.settings.startItem >= this.settings.maxItemDisplay-1) {
 +
this.settings.startItem = currentIndex - this.settings.maxItemDisplay+2;
 +
if (this.settings.startItem < 0) this.settings.startItem = 0;
 +
if (this.settings.startItem >this.slides.length-this.settings.maxItemDisplay) {
 +
this.settings.startItem = this.slides.length-this.settings.maxItemDisplay;
 +
}
 +
}
 +
this.navigatorInner.stop().animate( eval('({'+this.navigratorStep[0]+':-'+this.settings.startItem*this.navigratorStep[1]+'})'),
 +
{duration:500, easing:'easeInOutQuad'} );
 +
},
 +
setNavActive:function( index, item ){
 +
if( (this.navigatorItems) ){
 +
this.navigatorItems.removeClass( 'active' );
 +
$(this.navigatorItems.get(index)).addClass( 'active' );
 +
this.navivationAnimate( this.currentNo );
 +
}
 +
},
 +
__getPositionMode:function( position ){
 +
if( position  == 'horizontal' ){
 +
return ['left', this.settings.navigatorWidth];
 +
}
 +
return ['top', this.settings.navigatorHeight];
 +
},
 +
__getDirectionMode:function(){
 +
switch( this.settings.direction ){
 +
case 'opacity': this.maxSize=0; return ['opacity','opacity'];
 +
default: this.maxSize=this.maxWidth; return ['left','width'];
 +
}
 +
},
 +
registerWheelHandler:function( element, obj ){
 +
element.bind('mousewheel', function(event, delta ) {
 +
var dir = delta > 0 ? 'Up' : 'Down',
 +
vel = Math.abs(delta);
 +
if( delta > 0 ){
 +
obj.previous( true );
 +
} else {
 +
obj.next( true );
 +
}
 +
return false;
 +
});
 +
},
 +
registerButtonsControl:function( eventHandler, objects, self ){
 +
for( var action in objects ){
 +
switch (action.toString() ){
 +
case 'next':
 +
objects[action].click( function() { self.next( true) } );
 +
break;
 +
case 'previous':
 +
objects[action].click( function() { self.previous( true) } );
 +
break;
 +
}
 +
}
 +
return this;
 +
},
 +
onProcessing:function( manual, start, end ){
 +
this.previousNo = this.currentNo + (this.currentNo>0 ? -1 : this.slides.length-1);
 +
this.nextNo = this.currentNo + (this.currentNo < this.slides.length-1 ? 1 : 1- this.slides.length);
 +
return this;
 +
},
 +
finishFx:function( manual ){
 +
if( manual ) this.stop();
 +
if( manual && this.settings.auto ){
 +
this.play( this.settings.interval,'next', true );
 +
}
 +
this.setNavActive( this.currentNo );
 +
},
 +
getObjectDirection:function( start, end ){
 +
return eval("({'"+this.directionMode[0]+"':-"+(this.currentNo*start)+"})");
 +
},
 +
fxStart:function( index, obj, currentObj ){
 +
if( this.settings.direction == 'opacity' ) {
 +
$(this.slides).stop().animate({opacity:0}, {duration: this.settings.duration, easing:this.settings.easing} );
 +
$(this.slides).eq(index).stop().animate( {opacity:1}, {duration: this.settings.duration, easing:this.settings.easing} );
 +
}else {
 +
this.wrapper.stop().animate( obj, {duration: this.settings.duration, easing:this.settings.easing} );
 +
}
 +
return this;
 +
},
 +
jumping:function( no, manual ){
 +
this.stop();
 +
if( this.currentNo == no ) return;
 +
var obj = eval("({'"+this.directionMode[0]+"':-"+(this.maxSize*no)+"})");
 +
this.onProcessing( null, manual, 0, this.maxSize )
 +
.fxStart( no, obj, this )
 +
.finishFx( manual );
 +
this.currentNo  = no;
 +
},
 +
next:function( manual , item){
 +
 
 +
this.currentNo += (this.currentNo < this.slides.length-1) ? 1 : (1 - this.slides.length);
 +
this.onProcessing( item, manual, 0, this.maxSize )
 +
.fxStart( this.currentNo, this.getObjectDirection(this.maxSize ), this )
 +
.finishFx( manual );
 +
},
 +
previous:function( manual, item ){
 +
this.currentNo += this.currentNo > 0 ? -1 : this.slides.length - 1;
 +
this.onProcessing( item, manual )
 +
.fxStart( this.currentNo, this.getObjectDirection(this.maxSize ), this )
 +
.finishFx( manual );
 +
},
 +
play:function( delay, direction, wait ){
 +
this.stop();
 +
if(!wait){ this[direction](false); }
 +
var self  = this;
 +
this.isRun = setTimeout(function() { self[direction](true); }, delay);
 +
},
 +
stop:function(){
 +
if (this.isRun == null) return;
 +
clearTimeout(this.isRun);
 +
            this.isRun = null;
 +
}
 +
})
 +
})(jQuery)
 +
</script>
 +
<style type="text/css">
 +
@charset "utf-8";
 +
/* CSS Document */
 +
.lof-slidecontent, .lof-slidecontent a {
 +
color:#FFF;
 +
}
 +
.lof-slidecontent a.readmore{
 +
color:#58B1EA;
 +
font-size:95%;
 +
 +
}
 +
.lof-slidecontent{
 +
position:relative;
 +
overflow:hidden;
 +
border:#F4F4F4 solid 1px;
 +
margin: 0 auto 30px auto;
 +
border-radius: 4px;
 +
}
 +
.lof-slidecontent .preload{
 +
height:100%;
 +
width:100%;
 +
position:absolute;
 +
top:0;
 +
left:0;
 +
z-index:100000;
 +
text-align:center;
 +
background:#FFF
 +
}
 +
.lof-slidecontent .preload div{
 +
height:100%;
 +
width:100%;
 +
background:transparent url(https://static.igem.org/mediawiki/2011/5/5d/IGEM_HokkaidoU_Index_Slideshow_Load-indicator.gif) no-repeat scroll 50% 50%;
 +
}
 +
.lof-main-outer{
 +
position:relative;
 +
height:100%;
 +
width:900px;
 +
z-index:3px;
 +
overflow:hidden;
 +
}
 +
 
 +
 
 +
/*******************************************************/
 +
.lof-main-item-desc{
 +
z-index:100px;
 +
position:absolute;
 +
bottom:55px;
 +
left:0px;
 +
background:url(https://static.igem.org/mediawiki/2011/e/eb/IGEM_HokkaidoU_Index_Slideshow_Bg_trans.png);
 +
height:1.5em;
 +
/* filter:0.7(opacity:60) */
 +
padding:10px;
 +
overflow: hidden;
 +
}
 +
.lof-main-item-desc p{
 +
margin:0 8px;
 +
padding:0 0;
 +
font-size: small;
 +
line-height: 1.2em;
 +
}
 +
.lof-main-item-desc h3{
 +
padding:0;
 +
margin:0;
 +
color: #fff;
 +
font-size: small;
 +
border-left: none;
 +
}
 +
.lof-main-item-desc h2{
 +
padding:0;
 +
margin:15px 0 0 0;
 +
color: #fff;
 +
border-bottom: none;
 +
font-weight:bold;
 +
}
 +
.lof-main-item-desc h3 a{
 +
margin:0;
 +
background:#C01F25;
 +
font-size:75%;
 +
padding:2px 3px;
 +
font-family:"Trebuchet MS",Trebuchet,Arial,Verdana,sans-serif;
 +
text-transform:uppercase;
 +
text-decoration:none
 +
}
 +
.lof-main-item-desc h3 a:hover{
 +
 
 +
text-decoration:underline;
 +
}
 +
.lof-main-item-desc h3 i {
 +
font-size:70%;
 +
}
 +
 
 +
/* main flash */
 +
ul.lof-main-wapper{
 +
/* margin-right:auto; */
 +
overflow:hidden;
 +
background:transparent url(https://static.igem.org/mediawiki/2011/5/5d/IGEM_HokkaidoU_Index_Slideshow_Load-indicator.gif) no-repeat scroll 50% 50%;
 +
padding:0px;
 +
margin:0;
 +
position:absolute;
 +
overflow:hidden;
 +
}
 +
 
 +
ul.lof-main-wapper li{
 +
overflow:hidden;
 +
padding:0px;
 +
margin:0px;
 +
float:left;
 +
position:relative;
 +
}
 +
.lof-opacity  li{
 +
position:absolute;
 +
top:0;
 +
left:0;
 +
float:inherit;
 +
}
 +
ul.lof-main-wapper li img{
 +
padding:0px;
 +
}
 +
 
 +
/* item navigator */
 +
.lof-navigator-wapper {
 +
position:absolute;
 +
bottom:10px;
 +
right:10px;
 +
background:url(https://static.igem.org/mediawiki/2011/b/b8/IGEM_HokkaidoU_Index_Slideshow_Transparent_bg.png) repeat;
 +
padding:5px 0px;
 +
}
 +
.lof-navigator-outer{
 +
position:relative;
 +
z-index:100;
 +
height:180px;
 +
width:310px;
 +
overflow:hidden;
 +
color:#FFF;
 +
float:left
 +
}
 +
ul.lof-navigator{
 +
top:0;
 +
padding:0;
 +
margin:0;
 +
position:absolute;
 +
width:100%;
 +
}
 +
ul.lof-navigator li{
 +
cursor:hand;
 +
cursor:pointer;
 +
list-style:none;
 +
padding:0;
 +
margin-left:0px;
 +
overflow:hidden;
 +
float:left;
 +
display:block;
 +
 
 +
text-align:center;
 +
 
 +
}
 +
ul.lof-navigator li img{
 +
border:#666 solid 3px;
 +
}
 +
ul.lof-navigator li.active img, ul.lof-navigator li:hover img {
 +
border:#A8A8A8 solid 3px;
 +
}
 +
.lof-navigator-wapper .lof-next,  .lof-navigator-wapper .lof-previous{
 +
display:block;
 +
width:22px;
 +
height:30px;
 +
color:#FFF;
 +
cursor:pointer;
 +
 
 +
}
 +
.lof-navigator-wapper .lof-next {
 +
float:left;
 +
text-indent:-999px;
 +
margin-right:5px;
 +
background:url(https://static.igem.org/mediawiki/2011/9/9c/IGEM_HokkaidoU_Index_Slideshow_Arrow-l.png) no-repeat right center;
 +
}
 +
.lof-navigator-wapper .lof-previous {
 +
float:left;
 +
text-indent:-999px;
 +
margin-left:5px;
 +
background:url(https://static.igem.org/mediawiki/2011/9/98/IGEM_HokkaidoU_Index_Slideshow_Arrow-r.png) no-repeat left center;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/header}}-->
 +
<html>
 +
<!--{{Team:HokkaidoU_Japan/css}}-->
 +
<html>
 +
<link href='http://fonts.googleapis.com/css?family=Terminal+Dosis|Miltonian|Josefin+Sans:400,700' rel='stylesheet' type='text/css'>
 +
 
 +
<!--{{Team:HokkaidoU_Japan/css/wikireset.css}}-->
 +
<html><style>
 +
/*
 +
* Reset iGEM Wiki CSS. Use this with Template:Team:HokkaidoU_Japan/css/reset.css
 +
*/
 +
#top-section{
 +
    position: absolute;
 +
    width: 100%;
 +
    height: 0;
 +
    border: none;
 +
}
 +
#top-section #menubar.left-menu{
 +
    position: absolute;
 +
    top: 0; left: 0;
 +
}
 +
#top-section #menubar.right-menu{
 +
    position: absolute;
 +
    top: 0; right: 0;
 +
}
 +
.editsection,
 +
#top-section #p-logo,
 +
#top-section #search-controls,
 +
#content h1.firstHeading,
 +
#content #bodyContent #contentSub,
 +
#content #bodyContent p,    /* holly crap, they have p element for script tag and no id and no class... */
 +
#catlinks{
 +
    display: none;
 +
}
 +
#globalWrapper,
 +
#globalWrapper #content{
 +
    background: none;
 +
    margin: 0;
 +
    padding: 0;
 +
    border: none;
 +
    width: auto;
 +
}
 +
 
 +
#footer-box {
 +
border-top: 1px solid #E4E4E4;
 +
border-right: 0px;
 +
border-bottom: 0px;
 +
border-left: 0px;
 +
}
 +
 
 +
/* because I hide p element in #bodyContent */
 +
#content #bodyContent div p{
 +
    display: block;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/css/components.css}}-->
 +
<html><style>
 +
.editsection{
 +
    font-size: 9px;
 +
    font-weight: normal;
 +
}
 +
.thumbinner{
 +
    -webkit-border-radius: 4px;
 +
    -moz-border-radius: 4px;
 +
    border-radius: 4px;
 +
}
 +
#menubar{
 +
    font-size: 10px;
 +
}
 +
#menubar ul{
 +
    margin: 0;
 +
    padding: 0;
 +
    width: auto;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/css/typography.css}}-->
 +
<html><style>
 +
@import url(http://fonts.googleapis.com/css?family=Terminal+Dosis);
 +
@import url(http://fonts.googleapis.com/css?family=Miltonian);
 +
@import url(http://fonts.googleapis.com/css?family=Josefin+Sans:400,700);
 +
body{
 +
    font-size: 100%;
 +
    font-family: 'Terminal Dosis', sans-serif;
 +
    background: #ffffff;
 +
    color: #3e3e3e;
 +
    letter-spacing: 0.1em;
 +
}
 +
a{
 +
    text-decoration: none;
 +
    color: #5a7bf8;
 +
}
 +
a:visited { color: #4f6ddc; }
 +
a:hover {
 +
    text-decoration: none;
 +
    color: #525bb8;
 +
}
 +
a:active { color: #4f6ddc; }
 +
 
 +
p{
 +
    margin: 0 0 1.5em 0;
 +
    text-indent: 0.5em;
 +
    line-height: 1.5em;
 +
}
 +
p:last-child {
 +
    margin-bottom: 0;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
    font-family: 'Josefin Sans', sans-serif;
 +
    font-weight: normal;
 +
    letter-spacing: 0.15em;
 +
    margin: 2em 0 1em 0;
 +
    padding: 0;
 +
}
 +
h1:first-child, h2:first-child, h3:first-child,
 +
h4:first-child, h5:first-child, h6:first-child {
 +
    margin-top: 0;
 +
}
 +
h1{
 +
    border-bottom: 1px solid #e4e4e4;
 +
    line-height: 1.2em;
 +
}
 +
h2{
 +
    border-bottom: 1px dotted #e4e4e4;
 +
}
 +
ul, ol{
 +
    margin: 1em 0 1em 0;
 +
    padding: 0 0 0 1em;
 +
}
 +
pre{
 +
    margin: 1em 0;
 +
    padding: 1em;
 +
    border: 1px solid #e4e4e4;
 +
    background: #f7f7f7;
 +
    -webkit-border-radius: 4px;
 +
    -moz-border-radius: 4px;
 +
    border-radius: 4px;
 +
}
 +
table{
 +
    border-collapse: collapse;
 +
    border: 1px solid #ababab;
 +
    font-size: 100%;
 +
}
 +
table th, table td{
 +
    padding: 0.4em 0.6em;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/css/toc.css}}-->
 +
<html><style>
 +
div.toc-wrapper {
 +
  display: block;
 +
  position: fixed;
 +
  top: 20%;
 +
  right: 0;
 +
  width: 300px;
 +
  height: 70%;
 +
  z-index: 10000;
 +
}
 +
 
 +
#toc {
 +
  display: block;
 +
  height: 100%;
 +
  overflow: auto;
 +
}
 +
 
 +
div.toc-tab {
 +
  background: #dedede url(/wiki/images/f/f8/HokkaidoU_toc_tab.png) left top no-repeat;
 +
  top: 20%;
 +
  right: 0;
 +
  width: 30px;
 +
  height: 120px;
 +
  display: block;
 +
  position: fixed;
 +
  z-index: 9999;
 +
  cursor: pointer;
 +
}
 +
 
 +
div.toc-wrapper div.close {
 +
  background: url(/wiki/images/7/73/HokkaidoU_toc_close.png) left top no-repeat;
 +
  top : 10px;
 +
  right : 20px;
 +
  width: 20px;
 +
  height: 20px;
 +
  display: block;
 +
  position: absolute;
 +
  cursor: pointer;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/css/layout.v1.css}}-->
 +
<html><style>
 +
*:after{
 +
    display: block;
 +
    clear: both;
 +
}
 +
body{
 +
    font-size: 76%;
 +
    background: #ffffff;
 +
}
 +
#hokkaidou-container{
 +
    width: 980px;
 +
    margin: 0 auto;
 +
    padding: 0;
 +
}
 +
#hokkaidou-header{
 +
    background: #ffffff url(https://static.igem.org/mediawiki/2011/2/2b/Hokkaidou_header.png) repeat-x;
 +
    margin-bottom: 10px;
 +
}
 +
#hokkaidou-footer{
 +
    margin-top: 30px;
 +
    padding-top: 15px;
 +
    border-top: 1px solid #e4e4e4;
 +
    text-align: center;
 +
}
 +
#hokkaidou-footer #hokkaidou-sponsors ul li{
 +
    display: inline-block;
 +
    margin: 10px;
 +
    list-style: none;
 +
}
 +
#hokkaidou-footer #hokkaidou-sponsors ul:after {content: "";}
 +
</style></html>
 +
<!--{Team:HokkaidoU_Japan/css/layout.logo.css}}-->
 +
<html><style>
 +
#hokkaidou-header{
 +
    position: relative;
 +
    height: 117px;
 +
}
 +
#hokkaidou-header #hokkaidou-logo h1 a{
 +
    display: block;
 +
    position: absolute;
 +
    top: 10px; left: 20px;
 +
    width: 400px;
 +
    height: 75px;
 +
    background: url(https://static.igem.org/mediawiki/2011/e/e5/Hokkaidou_logo.png) no-repeat;
 +
    border: none;
 +
    text-indent: -10000px;
 +
    outline: none;
 +
}
 +
#hokkaidou-header #hokkaidou-logo h1 a:hover {
 +
    background-position: 0 -76px;
 +
}
 +
#hokkaidou-header #hokkaidou-logo h1 a:active {
 +
    background-position: 0 -152px;
 +
}
 +
#hokkaidou-header #hokkaidou-logo p{
 +
    display: none !important;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/css/layout.navigation.css}}-->
 +
<html><style>
 +
#hokkaidou-header #hokkaidou-navigation ul{
 +
    position: absolute;
 +
    top: 10px; right: 0;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li{
 +
    float: left;
 +
    list-style: none;
 +
    margin: 0;
 +
    text-align: center;
 +
    width: 60px;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li a,
 +
#hokkaidou-header #hokkaidou-navigation ul li strong.selflink{
 +
    display: inline-block;
 +
    width: 30px; height: 30px;
 +
    background: url(https://static.igem.org/mediawiki/2011/4/4b/Hokkaidou_menu.png) no-repeat;
 +
    border: none;
 +
    outline: none;
 +
    text-indent: -10000px;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a{ background-position: 0 0; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a:hover { background-position: -30px 0; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home strong.selflink{ background-position: -60px 0; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a{ background-position: 0 -30px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a:hover { background-position: -30px -30px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project strong.selflink{ background-position: -60px -30px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a{ background-position: 0 -60px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a:hover { background-position: -30px -60px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol strong.selflink{ background-position: -60px -60px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a{ background-position: 0 -90px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a:hover { background-position: -30px -90px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts strong.selflink{ background-position: -60px -90px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a{ background-position: 0 -120px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a:hover { background-position: -30px -120px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook strong.selflink{ background-position: -60px -120px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a{ background-position: 0 -150px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a:hover { background-position: -30px -150px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety strong.selflink{ background-position: -60px -150px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a{ background-position: 0 -180px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a:hover { background-position: -30px -180px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice strong.selflink{ background-position: -60px -180px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a{ background-position: 0 -210px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a:hover { background-position: -30px -210px; }
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team a:active,
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team strong.selflink{ background-position: -60px -210px; }
 +
 
 +
/* i couldn't be bothered to add text on picture... lol */
 +
#hokkaidou-header #hokkaidou-navigation ul li:after {
 +
    display: inline-block;
 +
    font-family: 'Josefin Sans', sans-serif;
 +
    font-size: 12px;
 +
    margin-top: 5px;
 +
    width: 100%;
 +
    line-height: 14px;
 +
}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-home:after {content: "Home";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-project:after {content: "Project";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-protocol:after {content: "Protocol";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-parts:after {content: "Parts";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-notebook:after {content: "Notebook";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-safety:after {content: "Safety";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-humanpractice:after {content: "Human Practice";}
 +
#hokkaidou-header #hokkaidou-navigation ul li#hokkaidou-menu-team:after {content: "Team";}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/css/layout.twocolumn.css}}-->
 +
<html><style>
 +
#hokkaidou-content{zoom: 1;}
 +
#hokkaidou-content:after { content: ""; }
 +
#hokkaidou-content #hokkaidou-left-content{
 +
    float: left;
 +
    width: 280px;
 +
    margin-right: 20px;
 +
}
 +
#hokkaidou-content #hokkaidou-left-content ul{
 +
    margin: 0;
 +
    padding: 0 1em;
 +
}
 +
#hokkaidou-content #hokkaidou-left-content ul li{
 +
    list-style: none;
 +
    margin: 0 0 1em 0;
 +
}
 +
#hokkaidou-content #hokkaidou-left-content ul li .hokkaidou-description{
 +
    font-size: small;
 +
    color: #8a8a8a;
 +
}
 +
#hokkaidou-content #hokkaidou-right-content{
 +
    float: right;
 +
    width: 680px;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/css/layout.index.css}}-->
 +
<html><style>
 +
#hokkaidou-index-menu{
 +
    margin: 0 0 30px 0 !important;
 +
    padding: 0 !important;
 +
    border: 1px solid #ababab;
 +
    -webkit-border-top-right-radius: 12px;
 +
    -moz-border-radius-topright: 12px;
 +
    border-top-right-radius: 12px;
 +
    -webkit-border-bottom-right-radius: 12px;
 +
    -moz-border-radius-bottomright: 12px;
 +
    border-bottom-right-radius: 12px;
 +
}
 +
#hokkaidou-index-menu li{
 +
    list-style: none;
 +
    margin: 0 !important;
 +
    padding: 0 !important;
 +
    background: -moz-radial-gradient(center 80px 45deg, circle cover, hsl(0, 0%, 100%) 0%, hsl(240, 5%, 70%) 100%);
 +
    overflow: hidden;
 +
    text-shadow: 0 1px 1px hsl(240, 10%, 80%);
 +
}
 +
#hokkaidou-index-menu li:first-child{
 +
    -webkit-border-top-right-radius: 12px;
 +
    -moz-border-radius-topright: 12px;
 +
    border-top-right-radius: 12px;
 +
}
 +
#hokkaidou-index-menu li:last-child{
 +
    -webkit-border-bottom-right-radius: 12px;
 +
    -moz-border-radius-bottomright: 12px;
 +
    border-bottom-right-radius: 12px;
 +
}
 +
 
 +
#hokkaidou-index-menu li a{
 +
    display: block;
 +
    padding: 10px 20px;
 +
    border-bottom: none;
 +
    color: #3b3b3b;
 +
    transition: background 0.5s ease-in-out;
 +
    -moz-transition-property: background;
 +
    -moz-transition-duration: 700ms;
 +
    background: #e4e4e4;
 +
    border-bottom: 1px solid #dedede;
 +
    border-top: 1px solid #f7f7f7;
 +
}
 +
#hokkaidou-index-menu li a{
 +
}
 +
#hokkaidou-index-menu li a:hover{
 +
    background: transparent none;
 +
}
 +
#hokkaidou-index-menu li a .hokkaidou-description{
 +
    display: block;
 +
    margin-left: 40px;
 +
}
 +
#hokkaidou-index-menu li a:before{
 +
    display: block;
 +
    float: left;
 +
    content: "";
 +
    width: 30px; height: 30px;
 +
    margin: 5px 10px 0 0;
 +
    background: url(https://static.igem.org/mediawiki/2011/4/4b/Hokkaidou_menu.png) no-repeat;
 +
}             
 +
#hokkaidou-index-menu li#hokkaidou-index-project a:before{ background-position: 0 -30px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-protocol a:before{ background-position: 0 -60px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-parts a:before{ background-position: 0 -90px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-notebook a:before{ background-position: 0 -120px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-safety a:before{ background-position: 0 -150px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-humanpractice a:before{ background-position: 0 -180px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-team a:before{ background-position: 0 -210px; }
 +
 
 +
#hokkaidou-index-menu li#hokkaidou-index-project a:hover:before{ background-position: -30px -30px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-protocol a:hover:before{ background-position: -30px -60px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-parts a:hover:before{ background-position: -30px -90px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-notebook a:hover:before{ background-position: -30px -120px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-safety a:hover:before{ background-position: -30px -150px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-humanpractice a:hover:before{ background-position: -30px -180px; }
 +
#hokkaidou-index-menu li#hokkaidou-index-team a:hover:before{ background-position: -30px -210px; }
 +
 
 +
#hokkaidou-official-team-logo{
 +
    text-align: center;
 +
}
 +
#hokkaidou-official-team-logo img{
 +
    display: block;
 +
    width: 240px;
 +
    height: 240px;
 +
    border: 1px solid #e4e4e4;
 +
    margin: 0 auto;
 +
}
 +
#hokkaidou-official-team-logo .hokkaidou-description{
 +
    font-size: small;
 +
    color: #ababab;
 +
}
 +
#hokkaidou-links ul{
 +
    margin: 20px 0 !important;
 +
    padding: 0 !important;
 +
}
 +
#hokkaidou-links ul {zoom: 1;}
 +
#hokkaidou-links ul:after {content: ""; }
 +
#hokkaidou-links ul li{
 +
    float: left;
 +
    display: block !important;
 +
    margin: 0 5px !important;
 +
    padding: 0 !important;
 +
}
 +
#hokkaidou-links ul li:first-child{
 +
    margin-left: 0 !important;
 +
}
 +
#hokkaidou-links ul li a{
 +
    display: block;
 +
    width: 32px;
 +
    height: 32px;
 +
    background: url(https://static.igem.org/mediawiki/2011/8/8b/IGEM_HokkaidoU_Index_Links.png) no-repeat;
 +
    text-indent: -10000px;
 +
}
 +
#hokkaidou-links ul li#hokkaidou-links-email a{ background-position: 0 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-facebook a{ background-position: -32px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-twitter a{ background-position: -64px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-wordpress a{ background-position: -96px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-youtube a{ background-position: -128px 0; }
 +
#hokkaidou-links ul li#hokkaidou-links-email{
 +
    display: block !important;
 +
    width: 32px !important;
 +
    height: 32px !important;
 +
    margin: 0 5px 0 0 !important;
 +
    padding: 0 !important;
 +
}
 +
#hokkaidou-links ul li#hokkaidou-links-email a{
 +
    display: block !important;
 +
    width: 100% !important;
 +
    height: 100% !important;
 +
    margin: 0 !important;
 +
    padding: 0 !important;
 +
    background: url(https://static.igem.org/mediawiki/2011/8/8b/IGEM_HokkaidoU_Index_Links.png) no-repeat !important;
 +
    text-indent: -10000px !important;
 +
}
 +
</style></html>
 +
<!--{{Team:HokkaidoU_Japan/header}}-->
 +
</html>
 +
 
 +
 
 +
 
 +
<div id="hokkaidou-container">
 +
    <div id="hokkaidou-header">
 +
        <div id="hokkaidou-logo">
 +
            <h1><html><a href="https://2011.igem.org/Team:HokkaidoU_Japan">HokkaidoU Japan</a></html></h1>
 +
            <p class="description">iGEM 2011 Team of Hokkaido University</p>
 +
        </div>
 +
        <div id="hokkaidou-navigation"><ul>
 +
            <li id="hokkaidou-menu-home">[[Team:HokkaidoU_Japan|Home]]</li>
 +
            <li id="hokkaidou-menu-team">[[Team:HokkaidoU_Japan/Team|Team]]</li>
 +
            <li id="hokkaidou-menu-project">[[Team:HokkaidoU_Japan/Project|Project]]</li>
 +
            <li id="hokkaidou-menu-parts">[[Team:HokkaidoU_Japan/Parts|Parts]]</li>
 +
            <li id="hokkaidou-menu-protocol">[[Team:HokkaidoU_Japan/Protocols|Protocols]]</li>
 +
            <li id="hokkaidou-menu-notebook">[[Team:HokkaidoU_Japan/Notebook|Notebook]]</li>
 +
            <li id="hokkaidou-menu-safety">[[Team:HokkaidoU_Japan/Safety|Safety]]</li>
 +
            <li id="hokkaidou-menu-humanpractice">[[Team:HokkaidoU_Japan/HumanPractice|Human Practice]]</li>
 +
        </ul></div>
 +
    </div>
 +
    <div id="hokkaidou-content">
 +
 
 +
<!--Javascript-->
 +
<html>
 +
<script type="text/javascript">
 +
    $(document).ready(function () {
 +
        var buttons = {
 +
            previous: $('#lofslidercontent45 .lof-previous'),
 +
            next: $('#lofslidercontent45 .lof-next')
 +
        };
 +
        $obj = $('#lofslidecontent45').lofJSidernews({
 +
            interval: 5000,
 +
            direction: 'opacitys',
 +
            easing: 'easeInOutExpo',
 +
            duration: 1200,
 +
            auto: true,
 +
            maxItemDisplay: 7,
 +
            startItem: 0,
 +
            navPosition: 'horizontal', // horizontal
 +
            navigatorHeight: 32,
 +
            navigatorWidth: 80,
 +
            mainWidth: 780,
 +
            buttons: buttons
 +
        });
 +
    });
 +
</script>
 +
</html>
 +
<!--Left-cntent-->
 +
<html>
 +
<div id="hokkaidou-left-content">
 +
    <ul id="hokkaidou-index-menu">
 +
        <li id="hokkaidou-index-team"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Team">Team
 +
            <span class="hokkaidou-description">
 +
                The HokkaidoU_Japan is formed by 16 students and 3 instructors
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-project"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Project">Project
 +
            <span class="hokkaidou-description">
 +
                We tried to further develop E. coli-based protein injection system which we started working on during iGEM 2010
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-parts"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Parts">Parts
 +
            <span class="hokkaidou-description">
 +
                Ready-to-inject backbone and Bsa I cloning system
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-protocol"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Protocols">Protocol
 +
            <span class="hokkaidou-description">
 +
                General protocols, Primers and methods of infection assay
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-notebook"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Notebook">Notebook
 +
            <span class="hokkaidou-description">
 +
                The record of our many failures and a bit of achievement
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-safety"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/Safety">Safety
 +
            <span class="hokkaidou-description">
 +
                Addressing safety concerns
 +
            </span>
 +
        </a>
 +
        </li>
 +
        <li id="hokkaidou-index-humanpractice"><a href="https://2011.igem.org/Team:HokkaidoU_Japan/HumanPractice">Human Practice
 +
            <span class="hokkaidou-description">
 +
                Science art gallery "BIO ART"
 +
            </span>
 +
        </a>
 +
        </li>
 +
    </ul>
 +
    <div id="hokkaidou-official-team-logo">
 +
        <img src="http://igemhokkaidou.files.wordpress.com/2011/04/igem-hokkaidou-logo-transparent.png?w=240&h=240#038;h=240" width="240" height="240" />
 +
        <span class="hokkaidou-description">iGEM HokkaidoU_Japan Official Team Logo</span>
 +
    </div>
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    <div id="hokkaidou-links">
 +
        <ul>
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            <li id="hokkaidou-links-email"><a href="mailto:igemhokkaidou@gmail.com">Email</a></li>
 +
            <li id="hokkaidou-links-facebook"><a href="http://www.facebook.com/iGEMHokkaidoU">Facebook</a></li>
 +
            <li id="hokkaidou-links-twitter"><a href="http://twitter.com/iGEM_HokkaidoU">Twitter</a></li>
 +
            <li id="hokkaidou-links-wordpress"><a href="http://igemhokkaidou.com">WordPress</a></li>
 +
            <li id="hokkaidou-links-youtube"><a href="http://www.youtube.com/user/iGEMHokkaidoU">YouTube</a></li>
 +
        </ul>
 +
    </div>
 +
</html>
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<html>
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</div>
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<div id="hokkaidou-right-content">
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    <div id="lofslidecontent45" class="lof-slidecontent">
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        <div style="display: none;" class="preload"><div></div></div>
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            <div class="lof-main-outer" style="width:900px; height:340px;">
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            <ul style="left: -4200px; width: 6300px;" class="lof-main-wapper">
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                <li>
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                    <img src="https://static.igem.org/mediawiki/2011/6/64/IGEM_HokkaidoU_Index_Slideshow_7.jpg">         
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>HokkaidoU_Japan 2011</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/8/8a/IGEM_HokkaidoU_Index_Slideshow_1.jpg">         
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                    <div class="lof-main-item-desc">
 +
                        <h2>Welcome to the BIOxART Gallery!!</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/b/b5/IGEM_HokkaidoU_Index_Slideshow_2.jpg">         
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Be careful not to contaminate...</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/0/01/IGEM_HokkaidoU_Index_Slideshow_3.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Meeting, meeting and MEETING!</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/d/d1/IGEM_HokkaidoU_Index_Slideshow_4.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Dr. <i>E. coli</i> get ready for the job</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/0/06/IGEM_HokkaidoU_Index_Slideshow_5.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>BIOxART, please feel free to look around</h2>
 +
                    </div>
 +
                </li>
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2011/7/74/IGEM_HokkaidoU_Index_Slideshow_6.jpg">           
 +
                    <div class="lof-main-item-desc">
 +
                        <h2>Experiment, experiment and EXPERIMENT!</h2>
 +
                    </div>
 +
                </li>
 +
            </ul>   
 +
        </div>
 +
        <div class="lof-navigator-wapper">
 +
            <div style="width: 320px; height: 32px;" class="lof-navigator-outer">
 +
                <ul style="width: 54px; left: -160px;" class="lof-navigator">
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/8/81/IGEM_HokkaidoU_Index_Slideshow_Thumb_7.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/f3/IGEM_HokkaidoU_Index_Slideshow_Thumb_1.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/a/aa/IGEM_HokkaidoU_Index_Slideshow_Thumb_2.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/f/fb/IGEM_HokkaidoU_Index_Slideshow_Thumb_3.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/e/e2/IGEM_HokkaidoU_Index_Slideshow_Thumb_4.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/9/93/IGEM_HokkaidoU_Index_Slideshow_Thumb_5.jpg"></li>
 +
                    <li class="" style="height: 32px; width: 80px;"><img src="https://static.igem.org/mediawiki/2011/2/21/IGEM_HokkaidoU_Index_Slideshow_Thumb_6.jpg"></li>
 +
                </ul>
 +
            </div>
 +
        </div>
 +
    </div>
 +
    <h2>Abstract</h2>
 +
    <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
 +
    <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
 +
    <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
 +
 
 +
 
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Latest revision as of 09:45, 8 August 2013

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  • HokkaidoU_Japan 2011

  • Welcome to the BIOxART Gallery!!

  • Be careful not to contaminate...

  • Meeting, meeting and MEETING!

  • Dr. E. coli get ready for the job

  • BIOxART, please feel free to look around

  • Experiment, experiment and EXPERIMENT!

Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

Retrieved from "http://2013.igem.org/Team:UCSF/Home1"