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Team:USP-Brazil/Results
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| - | + | <p style="padding-top: 50px; margin-left: -40px;"><img src="https://static.igem.org/mediawiki/2013/d/d1/TitleUSPBrResults.png" width="117" height="47" alt="Results" /></p> | |
| - | + | ||
<h3>Overview</h3> | <h3>Overview</h3> | ||
<p>Besides designing a bioengineered microorganism that could help solve the methanol poisoning | <p>Besides designing a bioengineered microorganism that could help solve the methanol poisoning | ||
| - | problem, the pragmatically goal on wet lab was to generate new data on P< | + | problem, the pragmatically goal on wet lab was to generate new data on P<sub>AOX1</sub> and P<sub>FLD1</sub> |
| - | + | transcriptional profiles, measuring the performance of kinetic parameters and input compatibility | |
using the standardization principles of synthetic biology [1]. We also tested some <i>P. pastoris</i> | using the standardization principles of synthetic biology [1]. We also tested some <i>P. pastoris</i> | ||
| - | grow and cultivation variables to verify how it would behave on detection conditions and on the | + | grow and cultivation variables to verify how it would behave on detection conditions and on the lyiophilization (freeze-drying) process.</p> |
| - | + | ||
<p>The main questions regarding on our experimental approach are:</p> | <p>The main questions regarding on our experimental approach are:</p> | ||
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<ul> | <ul> | ||
<li>How strong are the AOX1 and FLD1 promoters?</li> | <li>How strong are the AOX1 and FLD1 promoters?</li> | ||
| - | <li>How relatively strong are the | + | <li>How relatively strong are the P<sub>AOX1</sub> variants?</li> |
| - | <li>How different alcohol sources will behave on interaction with wild | + | <li>How different alcohol sources will behave on interaction with wild P<sub>AOX1</sub> and variants? (Activation? Repression?)</li> |
| - | <li>Will | + | <li>Will P<sub>FLD1</sub> be activated or repressed by inputs other than mentioned on literature (methylamine and methanol)?</li> |
<li>How long is the time delay response of these promoters in a system with RFP?</li> | <li>How long is the time delay response of these promoters in a system with RFP?</li> | ||
| - | <li>Have our Pichia codon-optimized RFP a better fluorescence than a non-optimized (BBa_E1010)?</li> | + | <li>Have our <i>Pichia</i> codon-optimized RFP a better fluorescence than a non-optimized (BBa_E1010)?</li> |
</ul> | </ul> | ||
| + | <br></br> | ||
</li> | </li> | ||
<li>Microbiology Questions | <li>Microbiology Questions | ||
<ul> | <ul> | ||
| - | Will P. pastoris survive on ethanol solutions?</li> | + | <li>Will <i>P. pastoris</i> survive on ethanol solutions?</li> |
| - | Will P. pastoris survive lyophilization? If survives, will the survival population be large enough to deliver the output signal at naked eye!?</li> | + | <li>Will <i>P. pastoris</i> survive lyophilization? If survives, will the survival population be large enough to deliver the output signal at naked eye!?</li> |
</ul> | </ul> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| + | <br></br> | ||
<p>Our team managed to answer some of these questions and is still working to completely deliver all of them with good quality. You may start checking our results on our <a href="https://2013.igem.org/Team:USP-Brazil/Results:Assemblies">Assemblies page</a>.</p> | <p>Our team managed to answer some of these questions and is still working to completely deliver all of them with good quality. You may start checking our results on our <a href="https://2013.igem.org/Team:USP-Brazil/Results:Assemblies">Assemblies page</a>.</p> | ||
| + | <p style="text-align:right"> | ||
| + | <span style="float:left"><a href="https://2013.igem.org/Team:USP-Brazil/Modeling"><i class="icon-circle-arrow-left"></i> Back to our mathematical models</a> | ||
| + | </span><a href="https://2013.igem.org/Team:USP-Brazil/HumanPractices">Check out our Human Practices results <i class="icon-circle-arrow-right"></i></a> | ||
| + | </p> | ||
Latest revision as of 01:14, 28 September 2013
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Overview
Besides designing a bioengineered microorganism that could help solve the methanol poisoning problem, the pragmatically goal on wet lab was to generate new data on PAOX1 and PFLD1 transcriptional profiles, measuring the performance of kinetic parameters and input compatibility using the standardization principles of synthetic biology [1]. We also tested some P. pastoris grow and cultivation variables to verify how it would behave on detection conditions and on the lyiophilization (freeze-drying) process.
The main questions regarding on our experimental approach are:
- BioBrick Characterization Questions
- How strong are the AOX1 and FLD1 promoters?
- How relatively strong are the PAOX1 variants?
- How different alcohol sources will behave on interaction with wild PAOX1 and variants? (Activation? Repression?)
- Will PFLD1 be activated or repressed by inputs other than mentioned on literature (methylamine and methanol)?
- How long is the time delay response of these promoters in a system with RFP?
- Have our Pichia codon-optimized RFP a better fluorescence than a non-optimized (BBa_E1010)?
- Microbiology Questions
- Will P. pastoris survive on ethanol solutions?
- Will P. pastoris survive lyophilization? If survives, will the survival population be large enough to deliver the output signal at naked eye!?
Our team managed to answer some of these questions and is still working to completely deliver all of them with good quality. You may start checking our results on our Assemblies page.
Back to our mathematical models Check out our Human Practices results
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