Team:UTK-Knoxville

From 2013.igem.org

(Difference between revisions)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
 +
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"><head>
 +
<title>Team:UK-Knoxville - 2013.igem.org</title>
 +
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270">
 +
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
 +
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
 +
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
 +
 +
<script>
 +
var skin="igem",
 +
stylepath="/wiki/skins",
 +
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 +
wgArticlePath="/$1",
 +
wgScriptPath="/wiki",
 +
wgScriptExtension=".php",
 +
wgScript="/wiki/index.php",
 +
wgVariantArticlePath=false,
 +
wgActionPaths={},
 +
wgServer="https://2013.igem.org",
 +
wgCanonicalNamespace="",
 +
wgCanonicalSpecialPageName=false,
 +
wgNamespaceNumber=0,
 +
wgPageName="Team:UTK-Knoxville",
 +
wgTitle="Team:UTK-Knoxville",
 +
wgAction="view",
 +
wgArticleId=462,
 +
wgIsArticle=true,
 +
wgUserName=null,
 +
wgUserGroups=null,
 +
wgUserLanguage="en",
 +
wgContentLanguage="en",
 +
wgBreakFrames=false,
 +
wgCurRevisionId=97422,
 +
wgVersion="1.16.5",
 +
wgEnableAPI=true,
 +
wgEnableWriteAPI=true,
 +
wgSeparatorTransformTable=["", ""],
 +
wgDigitTransformTable=["", ""],
 +
wgMainPageTitle="Main Page",
 +
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2013.igem.org", "5": "2013.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 +
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2013.igem.org": 4, "2013.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
 +
wgSiteName="2013.igem.org",
 +
wgCategories=[],
 +
wgRestrictionEdit=[],
 +
wgRestrictionMove=[];
 +
 +
function MM_preloadImages() { //v3.0
 +
  var d=document; if(d.images){ if(!d.MM_p) d.MM_p=new Array();
 +
    var i,j=d.MM_p.length,a=MM_preloadImages.arguments; for(i=0; i<a.length; i++)
 +
    if (a[i].indexOf("#")!=0){ d.MM_p[j]=new Image; d.MM_p[j++].src=a[i];}}
 +
}
 +
        </script>               
 +
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
<!-- Head Scripts -->
 +
<script src="/wiki/skins/common/ajax.js?270"></script>
 +
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
    <style type="text/css">
 +
<!--
 +
.mediawiki.ltr.ns-0.ns-subject.page-Team_UTK-Knoxville p {
 +
font-family: Arial, Helvetica, sans-serif;
 +
}
 +
.mediawiki.ltr.ns-0.ns-subject.page-Team_UTK-Knoxville #navi ul li {
 +
font-family: Arial, Helvetica, sans-serif;
 +
}
 +
.mediawiki.ltr.ns-0.ns-subject.page-Team_UTK-Knoxville p #docs-internal-guid-250b94f8-5ac6-52cd-1e8e-630db6d99c92 {
 +
font-family: Arial, Helvetica, sans-serif;
 +
}
 +
-->
 +
    </style>
 +
</head>
 +
 +
<body class="mediawiki  ltr ns-0 ns-subject page-Team_UTK-Knoxville">
 +
<!--
 +
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
 +
<!-- start content -->
 +
<p>
 +
 +
 +
<style>
 +
h1.firstHeading { display: none; }
 +
 +
p {text-align: justify;}
 +
 +
a:link { color: #00b0e6; text-decoration: none}
 +
a:visited { color:#00b0e6; text-decoration: none}
 +
a:hover { color:#f29400; text-decoration: none}
 +
a:active { color:#f29400; text-decoration: none}
 +
 +
#bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
 +
 +
table#team_members { text-align: justify; border: 0; }
 +
table#team_members h2, table#team_members h3 { clear: both; }
 +
 +
 +
/*-----------------------------------------------------------------------------------------------*/
 +
div.MenuBar ul li ul.DropDownMenu {
 +
  display: none; /* Hides all drop-down menus. */
 +
 +
}
 +
/*
 +
li:hover works in IE7 and FF2.
 +
a:hover works in IE6 and FF2.
 +
a:hover breaks li:hover in FF2.
 +
*/
 +
div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
 +
  display: none; /* Hides all side menus. */
 +
}
 +
/*------------------------------------------------------------------------------------- Menu Bar */
 +
div.MenuBar {
 +
  font: arial, helvetica, sans-serif;
 +
  height: 30px;
 +
  width: 910px;
 +
  /*width: 100%*/
 +
  margin: 0;
 +
  border-top: 0;
 +
  border-right: 0;
 +
  border-left: 0;
 +
  padding: 0;
 +
  background: black;
 +
 
 +
}
 +
div.MenuBar ul {
 +
  font: arial, helvetica, sans-serif;
 +
  text-align: center;
 +
  list-style-type: none;
 +
  margin: 0.5em auto;
 +
  border: 0;
 +
  padding: 0;
 +
  background: black;
 +
}
 +
div.MenuBar ul li {
 +
  font: arial, helvetica, sans-serif;
 +
  display: block;
 +
  padding: 0;
 +
  margin: 0;
 +
  font-size: 1.3em;
 +
  float: left;
 +
  background: black;
 +
  text-align: center;
 +
  width: 107px;
 +
  position: relative; /* Sets the positioning context for each drop-down menu. */
 +
}
 +
 +
div.MenuBar ul li a {
 +
  font: arial, helvetica, sans-serif;
 +
  display: block;
 +
  background: black;
 +
  height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
 +
  color: #ffffff;
 +
  padding-top: 4px;
 +
  padding-bottom: 4px;
 +
  padding-left: 1em; /* Sets the left space between top-level items. */
 +
  padding-right: 1em; /* Sets the right space between top-level items. */
 +
  text-decoration: none;
 +
}
 +
 +
/*------------------------------------------------------------------------------ Drop-Down Menus */
 +
div.MenuBar ul li:hover ul.DropDownMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu {
 +
  display: block;
 +
  width: 10em; /* Drop-down menu width.
 +
                  Use MenuTailor.css to customize. */
 +
  height: 1em;
 +
  padding: 1px; /* Sets the drop-down menu "effective border" width. */
 +
  position: absolute;
 +
  top: 23px; /* Places the drop-down menu under the menu bar.
 +
                Keep it sync with the menu bar height. */
 +
  left: 0; /* Aligns the drop-down menu to its top-level item. */
 +
  background-color: black; /* Selected item. */
 +
  color: #FFFFFF;
 +
 +
}
 +
div.MenuBar ul li:hover ul.DropDownMenu li a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a {
 +
  width: 10em; /* Keep it sync with the drop-down menu width.
 +
                            Use MenuTailor.css to customize. */
 +
  height: 1em;
 +
  padding-left: 0;
 +
  padding-right: 0;
 +
  background-color: black; /* Selected item. */
 +
  color: #FFFFFF;
 +
}
 +
ul.DropDownMenu li a span {
 +
  display: block;
 +
  padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
 +
  padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
 +
  text-align: right; /* Aligns the >> symbol to the right. */
 +
}
 +
ul.DropDownMenu li a span span {
 +
  float: left; /* Aligns the text (back) to the left. */
 +
  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
 +
  height: 20px;
 +
  color: #FFFFFF;
 +
}
 +
/*----------------------------------------------------------------------------------- Side Menus */
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
 +
  display: block;
 +
  width: 11em; /* Side menu width.
 +
                  Use MenuTailor.css to customize. */
 +
  padding: 1px; /* Sets the side menu "effective border" width. */
 +
  position: absolute;
 +
  top: -1px; /* Aligns the side menu to its drop-down menu item.
 +
                Keep it sync with the side menu "effective border" width. */
 +
  left: 13em; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width.
 +
                            Use MenuTailor.css to customize. */
 +
}
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
 +
  width: 11em; /* Keep it sync with the side menu width.
 +
                            Use MenuTailor.css to customize. */
 +
  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
 +
  left: 13em; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width.
 +
                            Use MenuTailor.css to customize. */
 +
}
 +
div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
 +
  padding-left: 1.5em; /* Sets the left space of each side menu item. */
 +
  padding-right: 0.5em; /* Sets the right space of each side menu item. */
 +
  text-align: left;
 +
  font: 12px arial, helvetica, sans-serif; /* Required for IE55. */
 +
  left: 13em; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width.
 +
                            Use MenuTailor.css to customize. */
 +
}
 +
/*----------------------------------------------------------------------------- Browser Specific */
 +
* html div.MenuBar ul li a {
 +
  float: left; /* Required for IE55 and IE6.
 +
                            Breaks O9.
 +
                            Hidden (* html) from non-IE browsers. */
 +
}
 +
* html ul.DropDownMenu li a:hover {
 +
  cursor: hand; /* Required for IE55.
 +
                  Hidden (* html) from non-IE browsers. */
 +
}
 +
ul.DropDownMenu li a:hover {
 +
  cursor: pointer; /* Required for IE6 and IE7.
 +
                      Hidding it (* html) from non-IE browsers breaks IE7!
 +
}
 +
* html div.MenuBar a:hover {
 +
  text-decoration: none; /* Required for IE55 and IE6.
 +
                            Hidden (* html) from non-IE browsers. */
 +
}
 +
* html div.MenuBar ul li table,
 +
* html div.MenuBar ul li table td {
 +
  border: 0; /* Required for IE55 and IE6.
 +
                Hidden (* html) from non-IE browsers. */
 +
}
 +
/*------------------------------------------------------------------------------- Default Colors */
 +
div.MenuBar {
 +
  background-color: Menu;
 +
  border-bottom: 1px solid ButtonShadow;
 +
}
 +
div.MenuBar a {
 +
  background-color: Menu; /* Top-level unselected items. */
 +
  color: MenuText;
 +
}
 +
div.MenuBar ul li:hover a,
 +
div.MenuBar ul li a:hover {
 +
  color: #ea7f16;
 +
  background-color: Highlight; /* Top-level selected item. */
 +
  color: HighlightText;
 +
}
 +
/*...............................................................................................*/
 +
div.MenuBar ul li:hover ul.DropDownMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu {
 +
  background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
 +
}
 +
div.MenuBar ul li:hover ul.DropDownMenu li a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a {
 +
  background-color: Menu;  Drop-down menu unselected items.
 +
                            Sets the drop-down menu "effective background" color. */
 +
  color: MenuText;
 +
}
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
 +
  background-color: Highlight; /* Drop-down menu selected item. */
 +
  color: HighlightText;
 +
}
 +
/*...............................................................................................*/
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
 +
  background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
 +
}
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
 +
  background-color: Menu; /* Side menu unselected items.
 +
                            Sets the side menu "effective background" color. */
 +
  color: MenuText;
 +
}
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
 +
  background-color: Highlight; /* Side menu selected item. */
 +
  color: HighlightText;
 +
}
 +
/*-----------------------------------------------------------------------------------------------*/
 +
/*Script-Free 3-Level Menu 1.2 Tailor
 +
  www.CesarDaniel.info
 +
/*-------------------------------------------------------------------------------------- General */
 +
body {
 +
  background: white;
 +
  color: black;
 +
  margin: 0;
 +
  border: 0;
 +
  padding: 0;
 +
}
 +
 +
 +
div.MenuBar {
 +
  font: 13px arial, helvetica, sans-serif;
 +
}
 +
div.MenuBar ul {
 +
  font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
 +
}
 +
/*--------------------------------------------------------------------------------------- Colors */
 +
div.MenuBar {
 +
  background-color: black; /* Selected item. */
 +
  color: #FFFFFF;
 +
  border-bottom: 1px solid ButtonShadow;
 +
}
 +
div.MenuBar a,
 +
div.MenuBar ul li:hover ul.DropDownMenu li a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a,
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
 +
  background-color: black; /* Selected item. */
 +
  color: #FFFFFF;
 +
}
 +
div.MenuBar ul li:hover a,
 +
div.MenuBar ul li a:hover,
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
 +
  background-color: #00b0e6; /* Selected item. */
 +
  color: #FFFFFF;
 +
}
 +
div.MenuBar ul li:hover ul.DropDownMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu,
 +
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
 +
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
 +
  background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
 +
}
 +
/*--------------------------------------------------------------------------------------- Widths */
 +
/*
 +
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
 +
  width: 11em; /* Drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
 +
  width: 12em; /* Drop-down menu width. */
 +
}
 +
 +
/*...............................................................................................*/
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
Side Menu #1
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
 +
  left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
 +
  width: 10em; /* Side menu width. */
 +
}
 +
/*...............................................................................................*/
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
Side Menu #2
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
 +
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
 +
  width: 10em; /* Side menu width. */
 +
}
 +
/*...............................................................................................*/
 +
/*
 +
Menu Bar 1
 +
Drop-Down Menu #2
 +
Side Menu #3
 +
*/
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
 +
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
 +
                            Keep it sync with the drop-down menu width. */
 +
}
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
 +
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
 +
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
 +
  width: 10em; /* Side menu width. */
 +
}
 +
/*...............................................................................................*/
 +
</style>
 +
<div id="header"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/thumb/2/20/Header2.jpg/800px-Header2.jpg" alt="Team UTK-Knoxville" width="910" height="234"></a></div>
 +
<div class="MenuBar" id="navi">
 +
<ul>
 +
<li>
 +
<a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Home
 +
<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
</li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Project<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
<ul class="DropDownMenu" id="MB1-DDM1">
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
 +
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/partsorigin"><span><span>Parts Origin</span></span></a></li>
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/construct"><span><span>Construct</span></span></a></li>
 +
</ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
    </li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
<ul class="DropDownMenu" id="MB1-DDM2">
 +
              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Submitted Parts -></span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
  <ul class="SideMenu" id="MB8-DDM8-SM7">
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/Aer"><span>Aer</span></a></li>
 +
                    <li><a href="https://2013.igem.org/Team:UTK-Knoxville/HemAT"><span>HemAT</span></a></li>
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/RcoM"><span>RcoM</span></a></li>
 +
                    <li><a href="https://2013.igem.org/Team:UTK-Knoxville/NarX"><span>NarX</span></a></li>
 +
                    <li><a href="https://2013.igem.org/Team:UTK-Knoxville/TodS"><span>TodS</span></a></li>
 +
                    <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>LasR</span></a></li>
 +
                    <li><a href="https://2013.igem.org/Team:UTK-Knoxville/EnvZ"><span>(EnvZ)</span></a></li>
 +
                    <li><a href="https://2013.igem.org/Team:UTK-Knoxville/NtrC"><span>(NtrC)</span></a></li>
 +
                    <li><a href="https://2013.igem.org/Team:UTK-Knoxville/primers"><span>Primers</span></a></li>
 +
  </ul>
 +
  <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Characterization</span></span></a></li>
 +
  </ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
    </li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Discussion<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
<ul class="DropDownMenu" id="MB1-DDM3">
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Background</span></span></a></li>
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Application</span></span></a></li>
 +
</ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
</li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:UTK-Knoxville/notebook" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
  <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
  <ul class="DropDownMenu" id="MB1-DDM5">
 +
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/notebook"><span><span>Daily Entries</span></span></a>
 +
    </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
 +
  </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Team Meetings</span></span></a>
 +
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Seminar on DETAILS</span></span></a>
 +
  </li></ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
</li>
 +
<li style="width: 160px">
 +
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">oUTreach<!--[if gt IE 6]><!--></a><!--<![endif]-->
 +
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 +
<ul class="DropDownMenu" id="MB1-DDM4">
 +
      <li><a href="https://2013.igem.org/Team:UTK-Knoxville/EastmanHITES"><span><span>Eastman HITES</span></span></a></li>
 +
                          <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Coming Soon</span></span></a></li>
 +
 +
  </ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
</li>
<li>
<li>
<a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Team<!--[if gt IE 6]><!--></a><!--<![endif]-->
<a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Team<!--[if gt IE 6]><!--></a><!--<![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
<ul class="DropDownMenu" id="MB1-DDM6">
<ul class="DropDownMenu" id="MB1-DDM6">
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/team"><span><span>Overview</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Advisors</span></span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/advisors"><span><span>Advisors</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Undergraduates</span></span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/undergraduates"><span><span>Undergraduates</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>University</span></span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/utk"><span><span>University</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION5</span></span></a></li>
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION6</span></span></a></li>
+
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
 +
    </li>
 +
<li>
 +
<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a>
</li>
</li>
 +
</ul>
 +
 +
</div>
 +
<h1><span class="mw-headline">Abstract</span></h1>
 +
<p>The major limitation in synthetic biology today is the lack of numerous, well characterized sensors.  Our project aims to provide a reliable scaffold to test potential sensing domains with unknown substrates.  We have created a standard platform to test a range of intracellular and transmembrane domains.  Positive results are reported with green fluorescent protein for easy identification which can be done with high throughput methods like 96 well plates.  We test our platform on sensors with interesting known responses.  The chimera proteins are also useful in creating signals orthogonal to the cell. </p>
 +
<p><img src="https://static.igem.org/mediawiki/2013/thumb/3/38/Tilebar2.png/800px-Tilebar2.png" alt="tilebar" width="910" height="200" border="0" usemap="#Map2">
 +
  <map name="Map2">
 +
    <area shape="rect" coords="9,5,198,196" href="https://2013.igem.org/Team:UTK-Knoxville/Aer" alt="Parts">
 +
    <area shape="rect" coords="228,7,435,197" href="https://2013.igem.org/Team:UTK-Knoxville/construct" alt="Contruct">
 +
    <area shape="rect" coords="469,5,675,196" href="https://2013.igem.org/Team:UTK-Knoxville/notebook" alt="Notebook">
 +
    <area shape="rect" coords="705,5,907,193" href="https://2013.igem.org/Team:UTK-Knoxville/primers" alt="Primers">
 +
  </map>
 +
</p>
 +
<h1>Project Description</h1>
 +
<p dir="ltr">The 2013 UTK-Knoxville iGem team is creating chimeric biosensors to provide fast and easy reporting of useful ligands, and also creating a proof of concept standard platform with which to characterize new biosensors.</p>
 +
<p dir="ltr">We are constructing chimeric proteins of five well studies sensors. &nbsp;The five sensors are Aer, HemAT, TodS, NarX, and RcoM. &nbsp;Aer senses redox states. &nbsp;HemAT directly senses O2 oxygen. &nbsp;TodS senses toluene as well as benzene, xylene, chlorotoluene, and other aromatics, most of which are toxic. &nbsp;NarX senses nitrate, which is used in explosives. &nbsp;RcoM senses CO, NO, CO2, and NO2, which are all toxic at high levels. &nbsp;Each of these chimera sensors circuit creates a fluorescent protein to report a positive signal and provide easy confirmation of the presence of the ligand. &nbsp;These sensors could be used in microfluidic blood, water pollution, and soil tests.</p>
 +
<p dir="ltr">The broader power of this project is that it can provide a way to characterize sensors with unknown stimuli. &nbsp;Currently around 25,000 sensors have been identified in genomes while only around 100 have been studied to find what stimuli they sense. &nbsp;Chimera proteins can provide a stable reporting platform to characterize the sensing domains without changing the reporting machinery (kinase, promoter, reporter gene). &nbsp;High throughput screens can then be done in conjunction with flow cytometry to understand how the sensors interact with an array of chemicals or other stimuli. &nbsp;This process traditionally takes years to complete. &nbsp;For example, E. coli has five chemotaxis receptors (sensors for ribose, aspartate, serine, peptides, and redox) and it took about 40 years to characterize these.</p>
 +
<p>
 +
Characterizing sensors with unknown stimuli would also shed light onto the desired environment of unculturable microorganisms through the evolved sensors present in their genome.</p>
 +
<p> [<a href="https://2013.igem.org/Team:UTK-Knoxville" class="external text" rel="nofollow">back</a>]</p>
 +
<p><a href="http://www.engr.utk.edu/cbe/"><img src="https://static.igem.org/mediawiki/2013/2/22/Footerutk.jpg" width="910" height="250" border="0" usemap="#Map">
 +
    <map name="Map">
 +
      <area shape="poly" coords="6,2,6,5" href="#">
 +
      <area shape="poly" coords="6,134,7,131,11,7,404,5,402,134" href="http://utk.edu" alt="UTK.edu">
 +
      <area shape="poly" coords="6,140" alt="UTK CBE">
 +
      <area shape="poly" coords="5,142,906,140,902,236,10,240" href="http://www.engr.utk.edu/cbe/" alt="UTK CBE">
 +
      <area shape="poly" coords="409,6,904,5,904,132,409,132" href="http://web.utk.edu/~ctrinh/" alt="Trinh Lab">
 +
    </map>
 +
</a></p>

Latest revision as of 22:03, 7 August 2013

Team:UK-Knoxville - 2013.igem.org

Abstract

The major limitation in synthetic biology today is the lack of numerous, well characterized sensors. Our project aims to provide a reliable scaffold to test potential sensing domains with unknown substrates. We have created a standard platform to test a range of intracellular and transmembrane domains. Positive results are reported with green fluorescent protein for easy identification which can be done with high throughput methods like 96 well plates. We test our platform on sensors with interesting known responses. The chimera proteins are also useful in creating signals orthogonal to the cell.

tilebar Parts Contruct Notebook Primers

Project Description

The 2013 UTK-Knoxville iGem team is creating chimeric biosensors to provide fast and easy reporting of useful ligands, and also creating a proof of concept standard platform with which to characterize new biosensors.

We are constructing chimeric proteins of five well studies sensors.  The five sensors are Aer, HemAT, TodS, NarX, and RcoM.  Aer senses redox states.  HemAT directly senses O2 oxygen.  TodS senses toluene as well as benzene, xylene, chlorotoluene, and other aromatics, most of which are toxic.  NarX senses nitrate, which is used in explosives.  RcoM senses CO, NO, CO2, and NO2, which are all toxic at high levels.  Each of these chimera sensors circuit creates a fluorescent protein to report a positive signal and provide easy confirmation of the presence of the ligand.  These sensors could be used in microfluidic blood, water pollution, and soil tests.

The broader power of this project is that it can provide a way to characterize sensors with unknown stimuli.  Currently around 25,000 sensors have been identified in genomes while only around 100 have been studied to find what stimuli they sense.  Chimera proteins can provide a stable reporting platform to characterize the sensing domains without changing the reporting machinery (kinase, promoter, reporter gene).  High throughput screens can then be done in conjunction with flow cytometry to understand how the sensors interact with an array of chemicals or other stimuli.  This process traditionally takes years to complete.  For example, E. coli has five chemotaxis receptors (sensors for ribose, aspartate, serine, peptides, and redox) and it took about 40 years to characterize these.

Characterizing sensors with unknown stimuli would also shed light onto the desired environment of unculturable microorganisms through the evolved sensors present in their genome.

[back]

UTK.edu UTK CBE UTK CBE Trinh Lab

Retrieved from "http://2013.igem.org/Team:UTK-Knoxville"