Team:UTK-Knoxville/11 June 2013
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li> | ||
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- | </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/partsorigin"><span><span>Parts Origin</span></span></a></li> |
- | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/construct"><span><span>Construct</span></span></a></li> |
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li> | ||
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- | <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white"> | + | <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">oUTreach<!--[if gt IE 6]><!--></a><!--<![endif]--> |
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li> | ||
- | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/EastmanHITES"><span><span>Eastman HITES</span></span></a></li> |
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li> | ||
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+ | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]--> | ||
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+ | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/team"><span><span>Overview</span></span></a></li> | ||
+ | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/advisors"><span><span>Advisors</span></span></a></li> | ||
+ | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/undergraduates"><span><span>Undergraduates</span></span></a></li> | ||
+ | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/utk"><span><span>University</span></span></a></li> | ||
+ | </ul> | ||
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<li> | <li> | ||
<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a> | <a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a> | ||
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</div> | </div> | ||
- | </ | + | <h1><img src="https://static.igem.org/mediawiki/2013/a/a1/CalendarNAVI.png" alt="NotebookNAVI" width="485" height="144" border="0" usemap="#Map2"> |
- | < | + | <map name="Map2"> |
+ | <area shape="rect" coords="112,4,385,138" href="https://2013.igem.org/Team:UTK-Knoxville/notebook" alt="NotebookRETURN"> | ||
+ | <area shape="rect" coords="7,8,102,136" href="https://2013.igem.org/Team:UTK-Knoxville/10_June_2013" alt="BACK"> | ||
+ | <area shape="rect" coords="392,8,483,140" href="https://2013.igem.org/Team:UTK-Knoxville/12_June_2013" alt="NEXT"> | ||
+ | </map> | ||
+ | </h1> | ||
+ | <h1><span class="mw-headline">June 11th 2013</span></h1> | ||
- | + | <p>Brandon re-plated transformations from yesterday and they did not work. Brandon will leave them in the incubator a little longer to watch for growth, but does not expect any. Still waiting to hear back from Eric on the status of the ligation/transformation he did. Last Friday (6/7) he picked up digested backbone, Aer-EnvZ, and OmpR, and planned to ligate the pieces and transform them in time to allow them to grow before today’s 5:00 meeting. Eric was able to confirm our Aer-EnvZ+OmpR construct in his lab. It has been confirmed in wildtype E. Coli. We need to prepare chemically competent ΔOmpR, ΔEnvZ, and ΔOmpR+ΔEnvZ transform our plasmid into.</p> | |
- | + | <ul> | |
- | + | <li>Whole group meeting</li> | |
- | + | <li>Aer/EnvZ is in the wild type.</li> | |
- | + | <li>Keep the website broad for now. But keep the offline files detailed.</li> | |
- | + | <li>Need to get the mutants for the cytoplasmic platform.</li> | |
- | + | <li>Can grow E coli on nitrates under anaerobic conditions as a negative control.</li> | |
- | + | <li>Design characterization experiments and controls for each construct.</li> | |
- | + | <li>For outreach on June 14th - videos, photos, short write up, demonstrations / activities done...</li> | |
- | + | </ul> | |
- | + | <p>[<a href="https://2013.igem.org/Team:UTK-Knoxville" class="external text" rel="nofollow">back</a>]</p> | |
- | + | <p><a href="http://www.engr.utk.edu/cbe/"><img src="https://static.igem.org/mediawiki/2013/2/22/Footerutk.jpg" width="910" height="250" border="0" usemap="#Map"> | |
- | + | <map name="Map"> | |
- | + | <area shape="poly" coords="6,2,6,5" href="#"> | |
- | + | <area shape="poly" coords="6,134,7,131,11,7,404,5,402,134" href="http://utk.edu" alt="UTK.edu"> | |
- | + | <area shape="poly" coords="6,140" alt="UTK CBE"> | |
- | + | <area shape="poly" coords="5,142,906,140,902,236,10,240" href="http://www.engr.utk.edu/cbe/" alt="UTK CBE"> | |
- | + | <area shape="poly" coords="409,6,904,5,904,132,409,132" href="http://web.utk.edu/~ctrinh/" alt="Trinh Lab"> | |
- | + | </map> | |
- | + | </a></p> | |
- | + | ||
- | <p>Brandon re-plated transformations from yesterday and they did not work. Brandon will leave them in the incubator a little longer to watch for growth, but does not expect any. | + | |
- | + | ||
- | Still waiting to hear back from Eric on the status of the ligation/transformation he did. Last Friday (6/7) he picked up digested backbone, Aer-EnvZ, and OmpR, and planned to ligate the pieces and transform them in time to allow them to grow before | + | |
- | + | ||
- | Eric was able to confirm our Aer-EnvZ+OmpR construct in his lab. It has been confirmed in wildtype E. Coli. We need to prepare chemically competent | + | |
- | <li> Whole group meeting </li> | + | |
- | <li> Aer/EnvZ is in the wild type.</li> | + | |
- | <li>Keep the website broad for now. | + | |
- | <li>Need to get the mutants for the cytoplasmic platform.</li> | + | |
- | <li>Can grow E coli on nitrates under anaerobic conditions as a negative control.</li> | + | |
- | <li>Design characterization experiments and controls for each construct.</li> | + | |
- | <li>For outreach on June 14th - videos, photos, short write up, demonstrations / activities done...</li> | + | |
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Latest revision as of 19:45, 30 July 2013
June 11th 2013
Brandon re-plated transformations from yesterday and they did not work. Brandon will leave them in the incubator a little longer to watch for growth, but does not expect any. Still waiting to hear back from Eric on the status of the ligation/transformation he did. Last Friday (6/7) he picked up digested backbone, Aer-EnvZ, and OmpR, and planned to ligate the pieces and transform them in time to allow them to grow before today’s 5:00 meeting. Eric was able to confirm our Aer-EnvZ+OmpR construct in his lab. It has been confirmed in wildtype E. Coli. We need to prepare chemically competent ΔOmpR, ΔEnvZ, and ΔOmpR+ΔEnvZ transform our plasmid into.
- Whole group meeting
- Aer/EnvZ is in the wild type.
- Keep the website broad for now. But keep the offline files detailed.
- Need to get the mutants for the cytoplasmic platform.
- Can grow E coli on nitrates under anaerobic conditions as a negative control.
- Design characterization experiments and controls for each construct.
- For outreach on June 14th - videos, photos, short write up, demonstrations / activities done...
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