Team:UTK-Knoxville/4 June 2013

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<h2> <span class="mw-headline" id="Monday_30_June_08"> Tuesday 04 June 13 </span></h2>
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<td><a href="https://2013.igem.org/UTK-Knoxville/1_May_2013" class="external text" rel="nofollow"><span class="day-active">1</span></a>
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<h3> <span class="mw-headline" id="Monday_30_June_08"> Tuesday 04 June 13 </span></h3>
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       <li> The Aer-EnvZ piece after OEPCR is the correct size.  After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR.  Brandon ran it at 58°C, 60°C, and 64°C.  Julie originally used 60°C, and the two primers are supposed to have melting temperatures of 58°C and 67°C.  Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.</li>
       <li> The Aer-EnvZ piece after OEPCR is the correct size.  After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR.  Brandon ran it at 58°C, 60°C, and 64°C.  Julie originally used 60°C, and the two primers are supposed to have melting temperatures of 58°C and 67°C.  Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.</li>
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Revision as of 03:58, 26 June 2013

Team:UK-Knoxville - 2013.igem.org

2013.igem.org

Tuesday 04 June 13