Team:UTK-Knoxville/4 June 2013

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<a href="../UTK-knoxville/2013.igem.org">2013.igem.org</a></p>
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<div id="header"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/thumb/2/20/Header2.jpg/800px-Header2.jpg" alt="Team UTK-Knoxville" width="910" height="234"></a></div>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Advisors</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Undergraduates</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>University</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION5</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
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</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li>
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</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/partsorigin"><span><span>Parts Origin</span></span></a></li>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/construct"><span><span>Construct</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
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                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li>
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      <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section1</span></span></a></li>
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      <li><a href="https://2013.igem.org/Team:UTK-Knoxville/EastmanHITES"><span><span>Eastman HITES</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li>
                               <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/advisors"><span><span>Advisors</span></span></a></li>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/undergraduates"><span><span>Undergraduates</span></span></a></li>
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<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a>
<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a>
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<td><a href="https://2013.igem.org/UTK-Knoxville/1_April_2013" class="external text" rel="nofollow"><span class="day-empty">1</span></a>
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<td><a href="https://2013.igem.org/UTK-Knoxville/2_April_2013" class="external text" rel="nofollow"><span class="day-empty">2</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/5_April_2013" class="external text" rel="nofollow"><span class="day-empty">5</span></a>
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<td><a href="https://2013.igem.org/UTK-Knoxville/8_April_2013" class="external text" rel="nofollow"><span class="day-empty">8</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/10_April_2013" class="external text" rel="nofollow"><span class="day-empty">10</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/11_April_2013" class="external text" rel="nofollow"><span class="day-empty">11</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/12_April_2013" class="external text" rel="nofollow"><span class="day-empty">12</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/13_April_2013" class="external text" rel="nofollow"><span class="day-empty">13</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/14_April_2013" class="external text" rel="nofollow"><span class="day-empty">14</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/20_April_2013" class="external text" rel="nofollow"><span class="day-active">20</span></a>
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</td><td><a href="https://2013.igem.org/UTK-Knoxville/21_April_2013" class="external text" rel="nofollow"><span class="day-empty">21</span></a>
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</td></tr><tr><td><a href="https://2013.igem.org/UTK-Knoxville/22_April_2013" class="external text" rel="nofollow"><span class="day-empty">22</span></a>
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<td><a href="https://2013.igem.org/UTK-Knoxville/23_April_2013" class="external text" rel="nofollow"><span class="day-empty">23</span></a>
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<h3> <span class="mw-headline" id="Monday_30_June_08"> Tuesday 04 June 13 </span></h3>
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   <li>The Aer-EnvZ piece after OEPCR is the correct size. After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR. Brandon ran it at 58&deg;C, 60&deg;C, and 64&deg;C. Julie originally used 60&deg;C, and the two primers are supposed to have melting temperatures of 58&deg;C and 67&deg;C. Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.</li>
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      <li> The Aer-EnvZ piece after OEPCR is the correct size. After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR. Brandon ran it at 58°C, 60°C, and 64°C. Julie originally used 60°C, and the two primers are supposed to have melting temperatures of 58°C and 67°C. Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.</li>
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Latest revision as of 20:00, 30 July 2013

Team:UK-Knoxville - 2013.igem.org

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June 4th 2013

  • The Aer-EnvZ piece after OEPCR is the correct size. After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR. Brandon ran it at 58°C, 60°C, and 64°C. Julie originally used 60°C, and the two primers are supposed to have melting temperatures of 58°C and 67°C. Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.

 

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