Team:UTK-Knoxville/HemAT

From 2013.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 46: Line 46:
wgRestrictionEdit=[],
wgRestrictionEdit=[],
wgRestrictionMove=[];
wgRestrictionMove=[];
-
</script>                 
+
 
 +
function MM_preloadImages() { //v3.0
 +
  var d=document; if(d.images){ if(!d.MM_p) d.MM_p=new Array();
 +
    var i,j=d.MM_p.length,a=MM_preloadImages.arguments; for(i=0; i<a.length; i++)
 +
    if (a[i].indexOf("#")!=0){ d.MM_p[j]=new Image; d.MM_p[j++].src=a[i];}}
 +
}
 +
        </script>                 
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
<!-- Head Scripts -->
<!-- Head Scripts -->
<script src="/wiki/skins/common/ajax.js?270"></script>
<script src="/wiki/skins/common/ajax.js?270"></script>
-
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
+
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
    <style type="text/css">
 +
<!--
 +
.mediawiki.ltr.ns-0.ns-subject.page-Team_UTK-Knoxville p {
 +
font-family: Arial, Helvetica, sans-serif;
 +
}
 +
.mediawiki.ltr.ns-0.ns-subject.page-Team_UTK-Knoxville #navi ul li {
 +
font-family: Arial, Helvetica, sans-serif;
 +
}
 +
.mediawiki.ltr.ns-0.ns-subject.page-Team_UTK-Knoxville #article-title-1 a {
 +
font-family: Arial, Helvetica, sans-serif;
 +
}
 +
-->
 +
    </style>
</head>
</head>
<body class="mediawiki  ltr ns-0 ns-subject page-Team_UTK-Knoxville">
<body class="mediawiki  ltr ns-0 ns-subject page-Team_UTK-Knoxville">
-
+
<!--
-
<!--
+
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
<!-- start content -->
<!-- start content -->
Line 416: Line 434:
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
-
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li>
+
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/partsorigin"><span><span>Parts Origin</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/construct"><span><span>Construct</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li>
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
+
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
-
</li>
+
</li>
<li>
<li>
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]-->
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]-->
Line 431: Line 447:
  <ul class="SideMenu" id="MB8-DDM8-SM7">
  <ul class="SideMenu" id="MB8-DDM8-SM7">
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/Aer"><span>Aer</span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/Aer"><span>Aer</span></a></li>
-
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/HemAT"><span>HemAT</span></a></li>
+
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>HemAT</span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/RcoM"><span>RcoM</span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>RcoM</span></a></li>
-
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/NarX"><span>NarX</span></a></li>
+
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>NarX</span></a></li>
-
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/TodS"><span>TodS</span></a></li>
+
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>TodS</span></a></li>
-
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/LasR"><span>LasR</span></a></li>
+
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>LasR</span></a></li>
-
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/EnvZ"><span>(EnvZ)</span></a></li>
+
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(EnvZ)</span></a></li>
-
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/NtrC"><span>(NtrC)</span></a></li>
+
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(NtrC)</span></a></li>
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/primers"><span>Primers</span></a></li>
                     <li><a href="https://2013.igem.org/Team:UTK-Knoxville/primers"><span>Primers</span></a></li>
  </ul>
  </ul>
  <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Characterization</span></span></a></li>
  <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Characterization</span></span></a></li>
-
  </ul>
+
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
     </li>
     </li>
Line 449: Line 465:
<ul class="DropDownMenu" id="MB1-DDM3">
<ul class="DropDownMenu" id="MB1-DDM3">
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Background</span></span></a></li>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li>
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Application</span></span></a></li>
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
Line 457: Line 473:
<a href="https://2013.igem.org/Team:UTK-Knoxville/notebook" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
<a href="https://2013.igem.org/Team:UTK-Knoxville/notebook" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
-
<ul class="DropDownMenu" id="MB1-DDM5">
+
  <!--[if lte IE 6]></td></tr></table></a><![endif]-->
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/notebook"><span><span>SECTION1 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
+
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
-
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
  <ul class="DropDownMenu" id="MB1-DDM5">
-
<ul class="SideMenu" id="MB1-DDM2-SM1">
+
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/notebook"><span><span>Daily Entries</span></span></a>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/notebook"><span>SECTION2</span></a></li>
+
    </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION3</span></a></li>
+
  </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Team Meetings</span></span></a>
-
</ul>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
</li>
+
-
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxvillecomingsoon"><span><span>SECTION4 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
<ul class="SideMenu" id="MB1-DDM2-SM2">
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION8</span></a></li>
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION9</span></a></li>
+
-
</ul>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
</li>
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION10 &gt;</span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
<ul class="SideMenu" id="MB1-DDM2-SM3">
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION11</span></a></li>
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION12</span></a></li>
+
-
</ul>
+
-
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
</li>
+
-
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Visualization</span></span></a>
+
-
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material &amp; Methods</span></span></a>
+
-
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Team Meetings</span></span></a>
+
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Seminar on DETAILS</span></span></a>
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Seminar on DETAILS</span></span></a>
-
</li></ul>
+
</li></ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
-
</li>
+
</li>
<li style="width: 160px">
<li style="width: 160px">
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">oUTreach<!--[if gt IE 6]><!--></a><!--<![endif]-->
<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">oUTreach<!--[if gt IE 6]><!--></a><!--<![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
-
<ul class="DropDownMenu" id="MB1-DDM4">
+
  <ul class="DropDownMenu" id="MB1-DDM4">
-
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Project Overview</span></span></a></li>
+
      <li><a href="https://2013.igem.org/Team:UTK-Knoxville/EastmanHITES"><span><span>Eastman HITES</span></span></a></li>
-
      <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section1</span></span></a></li>
+
                          <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Coming Soon</span></span></a></li>
-
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section2</span></span></a></li>
+
-
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section3</span></span></a></li>
+
-
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section4</span></span></a></li>
+
-
                              <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Section5</span></span></a></li>
+
-
</ul>
+
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
</li>
</li>
Line 514: Line 503:
</ul>
</ul>
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
-
  </li>
+
</li>
<li>
<li>
<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a>
<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a>
Line 529: Line 518:
     <area shape="poly" coords="342,49,339,69,372,73,376,54" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#T7promoterDETAILS" alt="T7">
     <area shape="poly" coords="342,49,339,69,372,73,376,54" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#T7promoterDETAILS" alt="T7">
     <area shape="poly" coords="552,385,567,393,581,346,584,309,579,247,550,172,504,117,420,66,380,56,378,72,432,91,494,132,549,210,568,298" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#NtrCDETAILS" alt="OmpR">
     <area shape="poly" coords="552,385,567,393,581,346,584,309,579,247,550,172,504,117,420,66,380,56,378,72,432,91,494,132,549,210,568,298" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#NtrCDETAILS" alt="OmpR">
-
     <area shape="poly" coords="547,397,561,405,559,408,556,416,549,429,539,445,527,461,512,478,494,494,468,513,449,523,442,507,468,493,493,472,509,456,524,437,534,421,541,409" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#HemATrDETAILS" alt="Aer">
+
     <area shape="poly" coords="547,397,561,405,559,408,556,416,549,429,539,445,527,461,512,478,494,494,468,513,449,523,442,507,468,493,493,472,509,456,524,437,534,421,541,409" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#HemATDETAILS" alt="Aer">
     <area shape="poly" coords="235,511,266,525,302,532,332,535,359,533,378,530,402,524,421,517,429,513,437,529,428,533,416,538,401,543,372,548,335,551,299,549,259,539,227,528" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#NtrBDETAILS" alt="EnvZ">
     <area shape="poly" coords="235,511,266,525,302,532,332,535,359,533,378,530,402,524,421,517,429,513,437,529,428,533,416,538,401,543,372,548,335,551,299,549,259,539,227,528" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#NtrBDETAILS" alt="EnvZ">
     <area shape="poly" coords="158,480,148,469,134,452,123,435,108,409,97,384,91,355,87,328,84,305,100,306,103,336,108,357,120,395,138,428,152,446,164,459,171,468" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#pBR322DETAILS" alt="pBR322">
     <area shape="poly" coords="158,480,148,469,134,452,123,435,108,409,97,384,91,355,87,328,84,305,100,306,103,336,108,357,120,395,138,428,152,446,164,459,171,468" href="https://2013.igem.org/Team:UTK-Knoxville/HemAT#pBR322DETAILS" alt="pBR322">
Line 536: Line 525:
<p>&nbsp;</p>
<p>&nbsp;</p>
<h3><span class="mw-headline" id="HemATDETAILS">HemAT</span></h3>
<h3><span class="mw-headline" id="HemATDETAILS">HemAT</span></h3>
 +
<p><img src="https://static.igem.org/mediawiki/2013/thumb/f/fd/HemAT_sensing_domain.png/647px-HemAT_sensing_domain.png" alt="HemAT" width="647" height="599"></p>
<p>Oxygen  gas, sensor FixL or HemAT (also need HemC).&nbsp;<a href="http://scop.berkeley.edu/sunid=111118">list of Direct Oxygen sensors </a>. <strong>FixL,</strong> was fused with <a href="http://www.sciencedirect.com/science/article/pii/S1570963902005551">5 different kinases</a> :  
<p>Oxygen  gas, sensor FixL or HemAT (also need HemC).&nbsp;<a href="http://scop.berkeley.edu/sunid=111118">list of Direct Oxygen sensors </a>. <strong>FixL,</strong> was fused with <a href="http://www.sciencedirect.com/science/article/pii/S1570963902005551">5 different kinases</a> :  
&ldquo;it was likely that the autophosphorylation activity of the  histidine kinase domain could not be regulated by the O2  association/dissociation in the sensor domain in the chimeric proteins&rdquo;  Intracellular &amp; redox sensor.&nbsp; <strong>O2 sensor, would be interesting.&nbsp; &amp; may also be able to sense&nbsp; CO in the absence of oxygen&nbsp; </strong>It seems like FixL is an O2 sensor.&nbsp; </p>
&ldquo;it was likely that the autophosphorylation activity of the  histidine kinase domain could not be regulated by the O2  association/dissociation in the sensor domain in the chimeric proteins&rdquo;  Intracellular &amp; redox sensor.&nbsp; <strong>O2 sensor, would be interesting.&nbsp; &amp; may also be able to sense&nbsp; CO in the absence of oxygen&nbsp; </strong>It seems like FixL is an O2 sensor.&nbsp; </p>
<p><a href="http://www.sciencedirect.com/science/article/pii/S0969212603001692">Sensing  domain is from 1-186</a>, its cytoplasmic, so it might not work on EnvZ.&nbsp; It also does not HAMP domain so we do not  think it would work on a <a href="http://aktuell.ruhr-uni-bochum.de/pm2012/pm00213.html.en">transmembrane kinase domain.</a></p>
<p><a href="http://www.sciencedirect.com/science/article/pii/S0969212603001692">Sensing  domain is from 1-186</a>, its cytoplasmic, so it might not work on EnvZ.&nbsp; It also does not HAMP domain so we do not  think it would work on a <a href="http://aktuell.ruhr-uni-bochum.de/pm2012/pm00213.html.en">transmembrane kinase domain.</a></p>
<p><a href="http://www.sciencedirect.com/science/article/pii/S1570963902005551">Chimeric  sensory kinases</a> containing O2 sensor domain of FixL and histidine kinase domain  from thermophile.</p>
<p><a href="http://www.sciencedirect.com/science/article/pii/S1570963902005551">Chimeric  sensory kinases</a> containing O2 sensor domain of FixL and histidine kinase domain  from thermophile.</p>
-
<p>Oxygen&#8208;Sensing  Histidine&#8208;Protein Kinases:<a href="http://www.sciencedirect.com/science/article/pii/S0076687907370109"> Assays of Ligand Binding and Turnover of Response‐Regulator Substrates</a>.</p>
+
<p>Oxygen&#8208;Sensing  Histidine&#8208;Protein Kinases:<a href="http://www.sciencedirect.com/science/article/pii/S0076687907370109"> Assays of Ligand Binding and Turnover of Response-Regulator Substrates</a>.</p>
<p>Heme-based  oxygen sensor protein FixL:<a href="http://www.sciencedirect.com/science/article/pii/S0531513102002364"> its structure and function</a>.</p>
<p>Heme-based  oxygen sensor protein FixL:<a href="http://www.sciencedirect.com/science/article/pii/S0531513102002364"> its structure and function</a>.</p>
<p><a href="http://www.uniprot.org/uniprot/P76129#section_seq">Direct oxygen</a><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1305375/"> sensor protein</a>.</p>
<p><a href="http://www.uniprot.org/uniprot/P76129#section_seq">Direct oxygen</a><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1305375/"> sensor protein</a>.</p>
Line 573: Line 563:
<p>&nbsp;</p>
<p>&nbsp;</p>
<p><s>This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. </s></p>
<p><s>This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. </s></p>
 +
<h4>Resources:</h4>
 +
<h4 id="article-title-1" itemprop="headline"><a href="http://www.pnas.org/content/98/16/9353.full">Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria</a></h4>
 +
<p><a href="http://www.google.com/patents/US7129329">Heme proteins hemAT-Hs and hemAT-Bs and their use in medicine and microsensors</a></p>
 +
<h4 id="article-title-2" itemprop="headline"><a href="http://www.jbc.org/content/277/16/13528.full">Resonance Raman and Ligand Binding Studies of the Oxygen-sensing Signal Transducer Protein HemAT from&nbsp;<em>Bacillus subtilis</em></a><em></em></h4>
 +
<p itemprop="headline"><a href="http://www.hawaii.edu/microbiology/Alam/research/index.html">Globin-Coupled Sensors and Protoglobins</a></p>
 +
<p itemprop="headline">&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p><br>
<p><br>

Latest revision as of 11:36, 5 August 2013

Team:UK-Knoxville - 2013.igem.org

HemAT

Aer plasmid KanR T7 OmpR Aer EnvZ pBR322

 

HemAT

HemAT

Oxygen gas, sensor FixL or HemAT (also need HemC). list of Direct Oxygen sensors . FixL, was fused with 5 different kinases : “it was likely that the autophosphorylation activity of the histidine kinase domain could not be regulated by the O2 association/dissociation in the sensor domain in the chimeric proteins” Intracellular & redox sensor.  O2 sensor, would be interesting.  & may also be able to sense  CO in the absence of oxygen  It seems like FixL is an O2 sensor. 

Sensing domain is from 1-186, its cytoplasmic, so it might not work on EnvZ.  It also does not HAMP domain so we do not think it would work on a transmembrane kinase domain.

Chimeric sensory kinases containing O2 sensor domain of FixL and histidine kinase domain from thermophile.

Oxygen‐Sensing Histidine‐Protein Kinases: Assays of Ligand Binding and Turnover of Response-Regulator Substrates.

Heme-based oxygen sensor protein FixL: its structure and function.

Direct oxygen sensor protein.

Amino Acid Sequence:
MLFKKDRKQETAYFSDSNGQQKNRIQLTNKHADVKKQLKMVRLGDAELYVLEQLQPLIQENIVNIVDAFYKNLDHESSL

MDIINDHSSVDRLKQTLKRHIQEMFAGVIDDEFIEKRNRIASIHLRIGLLPKWYMGAFQELLLSMIDIYEASITNQQELLKA

IKATTKILNLEQQLVLE

 

KanR

KanR provides kanamycin resistance to colonies with successful transformations.

 

pBR322

The pBR322 ori is a low copy origin of replication derived from

 

 

NtrB

Details for NtrB coming soon.Details for NtrB coming soon.Details for NtrB coming soon.

 

NtrC

Details for NtrC coming soon.Details for NtrC coming soon.Details for NtrC coming soon.

 

T7 Promoter

The T7 promoter is constitutively expressed by the phage T7 RNA polymerase. The polymerase is orthogonal to the wildtype E. coli promoters.

 

This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.

Gel samples

Gel Sample

This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.

 

Flow sample

FLowsample

 

This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go. This is where the deatils of Aer will go.

Resources:

Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria

Heme proteins hemAT-Hs and hemAT-Bs and their use in medicine and microsensors

Resonance Raman and Ligand Binding Studies of the Oxygen-sensing Signal Transducer Protein HemAT from Bacillus subtilis

Globin-Coupled Sensors and Protoglobins

 

 


[back]