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From 2013.igem.org

2013.igem.org

Primers

Name	Sequence	Melting Temp.(°C)	Function/Comment	Status
General (Transmembrane)
EnvZ_r	aaaaaactcgagttaggatccTTACCCTTCTTTTGTCGTGCC	57	Lowercase part has restriction sites: XhoI-ctcgag BamHI-ggatcc	In
OmpR_r	AAAAAACTCGAGAGGGGATCtcatgctttagagccgtccgg	60	Restriction sites: XhoI-ctcgag BamHI-ggatcc	In
OmpR_f	aaaaaaCATATGCAAGAGACCTACAAGATTCTG	53	Restriction site: NdeI-catatg	In
Aer
Aer_f	aaaaaagaattctaaagatctaaggagatataATGTCTTCTCATCCGTATGTC	53	Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct	In 5/30
Aer-EnvZ_1r	GCGCAAGTCGTGACTTACCCatttctgacactggacacctgg	58	Overlapping PCR primers	In 5/30
Aer-EnvZ_1f	ccaggtgtccagtgtcagaaatGGGTAAGTCACGACTTGCGC	59	Overlapping PCR primers	In 5/30
NarX
NarX_F				We can order both sets of primers (transmembrane region from either protein)
NarX_R
Nar-EnvZ_R
HemAT
HemAT_F	aaaaaagaattctaaagatctaaggagatataTTGTTATTTAAAAAAGACAGAAAACAAG	52	Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct	Ordered 6/12
HemAT_R	ggcgctggttatccatcggactggttgtactcgctttgaaacg	58	Overlapping PCR primers	Ordered 6/12
HemAT_NtrB_F	cgtttcaaagcgagtacaaccagtccgatggataaccagcgcc	61	Overlapping PCR primers	Ordered 6/12
TodS
RcoM
RcoM_F	aaaaaagaattctaaagatctaaggagatataTTAGTTTTTACTTGCTTCAGTTGG	54	Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct	Ordered 6/12
RcoM_R	ggcgctggttatccatcggaatgtttaaatattttgcaattgcttc	52	Overlapping PCR primers	Ordered 6/12
RcoM_NtrB_F	gaagcaattgcaaaatatttaaacattccgatggataaccagcgcc	61	Overlapping PCR primers	Ordered 6/12
LasR
Cytoplasmic Construct
NtrC_F	aaaaaacatatgatgcaacgagggatagtctgg	58	Restriction site: NdeI-catatg	Ordered 6/12
NtrC_R	agatccgagctggggatggagtgactcgagaaaaaa	57	XhoI-ctcgag	Ordered 6/12
NtrB_R	aaaaaactcgagttaggatccTTATTTCCTGATAGGCAGGTAAAC	54	Lowercase part has restriction sites: XhoI-ctcgag BamHI-ggatcc	Ordered 6/12
The backbone will be cut with NedI on the OmpR and NtrC side and BamHI on the EnvZ and NtrB side

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