Team:UT Dallas/Parts

From 2013.igem.org

(Difference between revisions)

Latest revision as of 00:41, 16 October 2013

Name	Type	Description	Designer	Length
BBa_K1214000	Signalling	ComC, Codes for Competence Signalling Peptide(CSP) in Streptococcus Mutans, Works with ComB and ComA	Alec Spinhirne	139
BBa_K1214005	Regulatory	ScrR, Sucrose Repressor. Represses the sucrose operon promoter pScr. Inactivated by sucrose	Alec Spinhirne	963
BBa_K1214006	Regulatory	pScr, Sucrose operon promoter. Regulated by sucrose operon repressor, ScrR	Alec Spinhirne	92
BBa_K1214007	Regulatory	pFru, Fructose operon promoter. Regulated by fructose operon repressor FruR	Alec Spinhirne	180
BBa_K1214008	Coding	FruR, Fructose operon repressor, Represses fructose operon promoter pFru.	Alec Spinhirne	1007
BBa_K1214009	Coding	Dex, Encodes for dextranase. Membrane-bound protein that binds to dextran.	Alec Spinhirne	2553
BBa_K1214010	Coding	NspC	Alec Spinhirne	1203
BBa_K1214011	Composite	pFru-RBS-GFP, Fructose operon promoter with GFP coding sequence downstream. Constitutively expresses GFP. Regulated by fructose operon repressor FruR (BBa_K1214008)	Alec Spinhirne	976
BBa_K1214012	Composite	pScr-RBS-GFP, Sucrose operon promoter with GFP coding sequence downstream. Should constitutively express GFP. Regulated by ScrR (BBa_K1214005)	Alec Spinhirne	888
BBa_K1214013	Composite	Promoter-RBS-FruR, Constitutive promoter with fructose operon repressor downstream. Should constitutively express FruR (BBa_K1214008)	Alec Spinhirne	1070
BBa_K1214014	Composite	Promoter-RBS-ScrR, Constitutive promoter with sucrose operon repressor downstream. Should constitutively express ScrR	Alec Spinhirne
BBa_K1214015	Composite	Promoter-RBS-FruR-TT-pFru-RBS-GFP, Constitutive promoter with fructose operon repressor downstream. Should repress pFru when fructose is not present. When fructose is added to system, GFP should be expressed	Alec Spinhirne
BBa_K1214016	Coding	RBS-NspC, Encodes for NspC. Expression should lead to the synthesis of norspermidine, an aid in breaking down biofilms	Alec Spinhirne	1264

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+{{:Team:UT_Dallas/Templates/template1}}
 <html>
+<div id="bottom-panel">
+<table border='1' cellspacing='1'>
+<tr>
+<td valign='top'><b>Name</b></td>
+<td valign='top'><b>Type</b></td>
+<td valign='top'><b>Description</b></td>
+<td valign='top'><b>Designer</b></td>
+<td valign='top'><b>Length</b></td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214000">BBa_K1214000</a></td>
+<td valign='top'>Signalling</td>
+<td valign='top'>ComC, Codes for Competence Signalling Peptide(CSP) in Streptococcus Mutans, Works with ComB and ComA</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>139</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214005">BBa_K1214005</a></td>
+<td valign='top'>Regulatory</td>
+<td valign='top'>ScrR, Sucrose Repressor. Represses the sucrose operon promoter pScr. Inactivated by sucrose </td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>963</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214006">BBa_K1214006</a></td>
+<td valign='top'>Regulatory</td>
+<td valign='top'>pScr, Sucrose operon promoter. Regulated by sucrose operon repressor, ScrR</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>92</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214007">BBa_K1214007</a></td>
+<td valign='top'>Regulatory</td>
+<td valign='top'>pFru, Fructose operon promoter. Regulated by fructose operon repressor FruR</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>180</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214008">BBa_K1214008</a></td>
+<td valign='top'>Coding</td>
+<td valign='top'>FruR, Fructose operon repressor, Represses fructose operon promoter pFru.</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>1007</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214009">BBa_K1214009</a></td>
+<td valign='top'>Coding</td>
+<td valign='top'>Dex, Encodes for dextranase. Membrane-bound protein that binds to dextran.</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>2553</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214010">BBa_K1214010</a></td>
+<td valign='top'>Coding</td>
+<td valign='top'>NspC</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>1203</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214011">BBa_K1214011</a></td>
+<td valign='top'>Composite</td>
+<td valign='top'>pFru-RBS-GFP, Fructose operon promoter with GFP coding sequence downstream.
+Constitutively expresses GFP. Regulated by fructose operon repressor FruR (BBa_K1214008)</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>976</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214012">BBa_K1214012</a></td>
+<td valign='top'>Composite</td>
+<td valign='top'>pScr-RBS-GFP, Sucrose operon promoter with GFP coding sequence downstream. Should constitutively express GFP. Regulated by ScrR (BBa_K1214005)</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>888</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214013">BBa_K1214013</a></td>
+<td valign='top'>Composite</td>
+<td valign='top'>Promoter-RBS-FruR, Constitutive promoter with fructose operon repressor downstream. Should constitutively express FruR (BBa_K1214008) </td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>1070</td>
+</tr>
+<tr>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214014">BBa_K1214014</a></td>
+<td valign='top'>Composite</td>
+<td valign='top'>Promoter-RBS-ScrR, Constitutive promoter with sucrose operon repressor downstream. Should constitutively express ScrR</td>
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>&nbsp;</td>
+</tr>
-<head>
+<tr>
-<style>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214015">BBa_K1214015</a></td>
-#content {
+<td valign='top'>Composite</td>
-       background: none;
+<td valign='top'>Promoter-RBS-FruR-TT-pFru-RBS-GFP, Constitutive promoter with fructose operon repressor downstream. Should repress pFru when fructose is not present. When fructose is added to system, GFP should be expressed</td>
-       box-shadow: 0px 0px 0px 0px;
+<td valign='top'>Alec Spinhirne</td>
+<td valign='top'>&nbsp;</td>
-#bodyContent {
+</tr>
-       margin-left: -1px;
-#footer-box {
-	width: 965px;
-	padding: 5px;
-	background-color: white;
-        margin: 0 auto;
-	border: 0px;
-        border-radius: 5px 5px 5px 5px;
-        box-shadow: 0px 2px 6px 2px #222;
-}
-</style>
-</head>
-<body class="space">
-  <div id="bottom-panel">
-    <table>
-      <tr>
-        <td>
-          <div style="width: 580px; margin-top:0px;">
-            <span id="abs-title">PARTS</span>
-            <span style="float: left;">
-              <p id="abs-text">
-<!-- Show=Undef -->
-<TABLE width='865px'><TR><TD width='50%'><B>UT_Dallas 2013 iGEM Team Parts Sandbox</B><TD width='35%'><TD width='15%' ALIGN='RIGHT'></TABLE></form><TABLE class='pgrouptable addborder' style='width: 925px;' cellpadding='0' cellspacing='0'><TR><TH colspan='2' style='width: 1em'  nowrap><A style='color:blue;text-decoration:none;'
-            href='http://parts.igem.org/wiki/index.php/Help:Availability_and _usefulness'></A><TH style='width: 7em;'>Name</TH><TH colspan=''>Type<TH colspan=''>Description<TH colspan=''>Designer<TH colspan=''>Length<TH class='blank_col'>
-<!-- ROW -->
-<!-- ROW -->
-<!-- ROW -->
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30233"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214000'>BBa_K1214000</A></TD><TD>Signalling</TD><TD>ComC</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>139</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30235"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214005'>BBa_K1214005</A></TD><TD>Regulatory</TD><TD>ScrR</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>963</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30236"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214006'>BBa_K1214006</A></TD><TD>Regulatory</TD><TD>pScr</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>92</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30237"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214007'>BBa_K1214007</A></TD><TD>Regulatory</TD><TD>pFru</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>180</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30238"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214008'>BBa_K1214008</A></TD><TD>Coding</TD><TD>FruR</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>1007</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30239"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214009'>BBa_K1214009</A></TD><TD>Coding</TD><TD>Dex</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>2553</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30241"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214010'>BBa_K1214010</A></TD><TD>Coding</TD><TD>NspC</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>1203</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30244"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214011'>BBa_K1214011</A></TD><TD>Composite</TD><TD>pFru-RBS-GFP</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>976</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30245"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214012'>BBa_K1214012</A></TD><TD>Composite</TD><TD>pScr-RBS-GFP</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>888</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30246"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214013'>BBa_K1214013</A></TD><TD>Composite</TD><TD>Promoter-RBS-Cra</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>1070</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30247"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214014'>BBa_K1214014</A></TD><TD>Composite</TD><TD>Promoter-RBS-ScrR</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>&nbsp;</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30248"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214015'>BBa_K1214015</A></TD><TD>Composite</TD><TD>Promoter-RBS-FruR-TT-pFru-RBS-GFP</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>&nbsp;</TD><TD class  ='blank_col'></TD></FORM>
-<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2013&amp;group=UT_Dallas&amp;Done=1" enctype="multipart/form-data">
-<input type="hidden" name="Editing" value="Editing"  /><input type="hidden" name="part" value="30363"  /><input type="hidden" name="pgroup" value="iGEM2013"  /><input type="hidden" name="group" value="UT_Dallas"  /><TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD><A class='noul_link part_link' href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214016'>BBa_K1214016</A></TD><TD>Coding</TD><TD>RBS-NspC</TD><TD width='100px'>Alec Spinhirne</TD><TD ALIGN='right'>1264</TD><TD class  ='blank_col'></TD></FORM></TABLE></FORM><BR><!-- end content -->
-        <div class='visualClear'></div>
-        </div>
-</div><!-- close pageWrapper -->
-         </div> <!-- close globalWrapper --><br><br>
-<font size=5><b>Experiments</b><br></font>
-<br>
-<font size=4> A) &nbsp;&nbsp;&nbsp;Promoter Evaluation<br></font>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We began by testing the relative strength of the promoters we added to the registry: pFru (BBa_K1214007) and pScr (BBa_K1214006). Our promoters and the medium constitutive promoter (BBa_K823014) were ligated with RBS-GFP, which served as our reporter molecule, and subsequently transformed into our chassis organism. The experiment we ran used glycerol stocks from this transformation, inoculated in 5mL of chloramphenicol broth overnight. The resulting cell cultures were then used for Flow cytometry analysis and microscope imaging.<br><br>
-<center><img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/flo_zps5109f505.png" border="0" alt=" photo flo_zps5109f505.png"/><br><br>
-<font size=3>Background Cp-GFP (left) versus GFP Expression at 395nm (right)<br>
-<img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/1_zps40df5709.png" border="0" alt=" photo 1_zps40df5709.png"/><img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/2_zpsb7ccdfae.png" border="0" alt=" photo 2_zpsb7ccdfae.png"/><br>
-Background pScr-GFP (left) versus GFP Expression at 395nm (right)<br>
-<img src="http://i826.photobucket.com/albums/zz188/lana_khazma/3_zpsebbfb6cc.png" border="0" alt=" photo 3_zpsebbfb6cc.png"/><img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/4_zpse136b2d5.png" border="0" alt=" photo 4_zpse136b2d5.png"/><br>
-Background pFru-GFP (left) versus GFP Expression at 395nm (right)<br></font>
-<img src="http://i826.photobucket.com/albums/zz188/lana_khazma/5_zpsa845b24d.png" border="0" alt=" photo 5_zpsa845b24d.png"/><img src="http://i826.photobucket.com/albums/zz188/lana_khazma/6_zps8f6d75ed.png" border="0" alt=" photo 6_zps8f6d75ed.png"/>
-<br><br></center>
-<font size=4>B) &nbsp;&nbsp;&nbsp;Repressor Evaluation<br></font>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Our team submitted two new repressors to the registry that work in conjunction with the aforementioned promoters. These repressors are Cra (BBa_K1214008) and ScrR (BBa_K1214005). In theory, these should be inducible repressors that repress their respective promoters. Cra (also known as FruR) will repress pFru until Fructose is introduced, wherein the repressor will unblock pFru and promotion will continue. The same relationship is expected between ScrR and pScr except that promotion is induced by sucrose in this case.<br>
-<font size=3>
-<blockquote>These repressors were transformed to make four systems, with the medium constitutive promoter indicated by Cp: <br></font>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. pFru-RBS-GFP-Cp-RBS-ScrR<br>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. pFru-RBS-GFP-Cp-RBS-Cra<br>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. pScr-RBS-GFP-Cp-RBS-ScrR<br>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;4. pScr-RBS-GFP-Cp-RBS-Cra<br></blockquote>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;These were plated, incubated overnight. Seeing as the plates were from a transformation, not all colonies are guaranteed to contain the completed system. Thus, the GFP expression on these plates would be less than the true GFP expression of the system and they were not used in our characterization.. <br>
- &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Colonies were then picked from these plates and inoculated in 5mL of chloramphenicol broth overnight. This inoculation was used to perform Flow cytometry analysis of the repressors. It should be noted that the promotion of the repressors is dictated by Cp more than pFru or pScr, and in conjunction with the aforementioned promoter data it can be seen that pFru may cause greater downstream promotion than Cp but was not used due to conflicts with repression. However, the data obtained shows the expected repression patterns, especially in relation to the specificity of the repressors.
-<br>
-[[File:FlowpScrrepression.png]]<br>
-[[File:FlowpFrurepression.png]]<br>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;These repressors are shown to greatly reduce the GFP seen in the systems without repressors. pFru expression of GFP under the repressor was reduced at least five-fold while pScr expression of GFP was reduced at least ten-fold. <br><br>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;There is also evidence of the repressor’s specificity shown in the difference between the promoter’s GFP expression depending on whether Cra or ScrR was in the system. The sucrose promoter was repressed approximately four times as much by ScR than by Cra. pFru was repressed roughly 20% more by Cra than by ScrR. This data supports the preexisting claim that the repressors are more specific to their particular promoters. This is important to consider when using our biobricks in a system.<br>
-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Preliminary tests on the inducibility of the repressors was performed but the data was inconclusive.<br>
-            </span>
+<tr>
-          </div>
+<td valign='top'><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1214016">BBa_K1214016</a></td>
-        </td>
+<td valign='top'>Coding</td>
-        <td>
+<td valign='top'>RBS-NspC, Encodes for NspC. Expression should lead to the synthesis of norspermidine, an aid in breaking down biofilms</td>
-          </div>
+<td valign='top'>Alec Spinhirne</td>
-        </td>
+<td valign='top'>1264</td>
-      </tr>
+</tr>
-    </table>
+</table>
-  </div>
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