Team:UT Dallas/week1

From 2013.igem.org

(Difference between revisions)

Latest revision as of 20:49, 23 September 2013

7/5/2013

Transformation

[GFP] Kit plate 5. Well 14k. Backbone pSB1A2. Resistance A. i5-5-1

[LacI] Kit plate 5. Well 20. Backbone pSB1A2. Resistance A. i5-5-2

7/6/2013

Colony Picking

I5-5-1 → 2 colonies → picked 2 → i5-5-3

→ i5-5-4

I5-5-2 → 0 colonies

7/7/2013

Mini Prep

I5-5-3: 67.4 ng/ul [GFP]

I5-5-4: 98.7 ng/ul [GFP]

I5-5-5: 408.7 ng/ul [+ confiol]

Test Digestion

	I5-5-31	I5-5-42
DNA	4	2
BSA	1	1
Buffer 4	1	1
EcoRI	1	1
Pst	1	1
H2O	2	4
	10	10

Revision as of 20:27, 23 September 2013 (view source) Alecspin (Talk \| contribs) ← Older edit		Latest revision as of 20:49, 23 September 2013 (view source) Alecspin (Talk \| contribs)
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-	<p class="c2"><span ~~class="c23"~~>7/5/2013 </span></p><p class="c2"><span ~~class="c23"~~>Transformation</span></p><p class="c2"><span>[GFP] Kit plate 5. Well 14k. Backbone pSB1A2. Resistance A. i5-5-1</span></p><p class="c2"><span>[LacI] Kit plate 5. Well 20. Backbone pSB1A2. Resistance A. i5-5-2</span></p><p class="~~c13 c2~~"><span></span></p><p class="c2"><span ~~class="c23"~~>7/6/2013</span></p><p class="c2"><span ~~class="c23"~~>Colony Picking</span></p><p class="c2"><span>I5-5-1 ~~? </span><span>~~ 2 colonies ? picked 2 ? i5-5-3</span></p><p class="c2"><span>                                     ? i5-5-4</span></p><p class="c2"><span>I5-5-2 ? 0 colonies</span></p><p class="~~c13 c2~~"><span></span></p><p class="c2"><span ~~class="c23"~~>7/7/2013</span></p><p class="c2"><span ~~class="c23"~~>Mini Prep</span></p><p class="c2"><span>I5-5-3: 67.4 ng/ul        [GFP]</span></p><p class="c2"><span>I5-5-4: 98.7 ng/ul        [GFP]</span></p><p class="c2"><span>I5-5-5: 408.7 ng/ul        [+ confiol]</span></p><p class="~~c13 c2~~"><span></span></p><p class="c2"><span ~~class="c23"~~>Test Digestion</span></p><a href="#" name="d2fd616f7186be08d9c7705395c2136d5ae85998"></a><a href="#" name="0"></a><table class="~~c29~~" cellpadding="0" cellspacing="0"><tbody><tr class="~~c36~~"><td class="~~c89~~"><p class="~~c0 c13~~"><span></span></p></td><td class="~~c89~~"><p class="c0"><span>I5-5-3</span><span class="~~c92~~">1</span></p></td><td class="~~c89~~"><p class="c0"><span>I5-5-4</span><span class="~~c92~~">2</span></p></td></tr><tr class="~~c68~~"><td class="~~c89~~"><p class="c0"><span>DNA</span></p></td><td class="~~c89~~"><p class="c0"><span>4</span></p></td><td class="~~c89~~"><p class="c0"><span>2</span></p></td></tr><tr class="~~c36~~"><td class="~~c89~~"><p class="c0"><span>BSA</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td></tr><tr class="~~c68~~"><td class="~~c89~~"><p class="c0"><span>Buffer 4</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td></tr><tr class="~~c36~~"><td class="~~c89~~"><p class="c0"><span>EcoRI</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td></tr><tr class="~~c68~~"><td class="~~c89~~"><p class="c0"><span>Pst</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td><td class="~~c89~~"><p class="c0"><span>1</span></p></td></tr><tr class="~~c68~~"><td class="~~c89~~"><p class="c0"><span>H</span><span class="~~c109~~">2</span><span>O</span></p></td><td class="~~c89~~"><p class="c0"><span>2</span></p></td><td class="~~c89~~"><p class="c0"><span>4</span></p></td></tr><tr class="~~c68~~"><td class="~~c89~~"><p class="~~c0 c13~~"><span></span></p></td><td class="~~c89~~"><p class="c0"><span>10</span></p></td><td class="~~c89~~"><p class="c0"><span>10</span></p></td></tr></tbody></table~~><p class="c13 c2"><span></span></p><p class="c2"><span class="c23"></span></p~~>	+	<p class="c2"><span>7/5/2013 </span></p><p class="c2"><span>Transformation</span></p><p class="c2"><span>[GFP] Kit plate 5. Well 14k. Backbone pSB1A2. Resistance A. i5-5-1</span></p><p class="c2"><span>[LacI] Kit plate 5. Well 20. Backbone pSB1A2. Resistance A. i5-5-2</span></p><p class="c1"><span></span></p><p class="c2"><span>7/6/2013</span></p><p class="c2"><span>Colony Picking</span></p><p class="c2"><span>I5-5-1 →  2 colonies → picked 2 → i5-5-3</span></p><p class="c2"><span>                                        → i5-5-4</span></p><p class="c2"><span>I5-5-2 → 0 colonies</span></p><p class="c1"><span></span></p><p class="c2"><span>7/7/2013</span></p><p class="c2"><span>Mini Prep</span></p><p class="c2"><span>I5-5-3: 67.4 ng/ul        [GFP]</span></p><p class="c2"><span>I5-5-4: 98.7 ng/ul        [GFP]</span></p><p class="c2"><span>I5-5-5: 408.7 ng/ul        [+ confiol]</span></p><p class="c1"><span></span></p><p class="c2"><span>Test Digestion</span></p><a href="#" name="d2fd616f7186be08d9c7705395c2136d5ae85998"></a><a href="#" name="0"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c37"><td class="c68"><p class="c1 c4"><span></span></p></td><td class="c68"><p class="c4 c2"><span>I5-5-3</span><span class="c97">1</span></p></td><td class="c68"><p class="c4 c2"><span>I5-5-4</span><span class="c97">2</span></p></td></tr><tr class="c56"><td class="c68"><p class="c4 c2"><span>DNA</span></p></td><td class="c68"><p class="c4 c2"><span>4</span></p></td><td class="c68"><p class="c4 c2"><span>2</span></p></td></tr><tr class="c37"><td class="c68"><p class="c4 c2"><span>BSA</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td></tr><tr class="c56"><td class="c68"><p class="c4 c2"><span>Buffer 4</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td></tr><tr class="c37"><td class="c68"><p class="c4 c2"><span>EcoRI</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td></tr><tr class="c56"><td class="c68"><p class="c4 c2"><span>Pst</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td><td class="c68"><p class="c4 c2"><span>1</span></p></td></tr><tr class="c56"><td class="c68"><p class="c4 c2"><span>H</span><span class="c107">2</span><span>O</span></p></td><td class="c68"><p class="c4 c2"><span>2</span></p></td><td class="c68"><p class="c4 c2"><span>4</span></p></td></tr><tr class="c56"><td class="c68"><p class="c1 c4"><span></span></p></td><td class="c68"><p class="c4 c2"><span>10</span></p></td><td class="c68"><p class="c4 c2"><span>10</span></p></td></tr></tbody></table>