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Team:Washington/HOMEPAGE PROTOCOL
From 2013.igem.org
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">8) DNA Sequencing</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">8) DNA Sequencing</a></li> | ||
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">9) Making Glycerol Frozen Stocks</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">9) Making Glycerol Frozen Stocks</a></li> | ||
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</ul> | </ul> | ||
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</p> | </p> | ||
Revision as of 01:40, 7 September 2013
Tips and Etiquette:
Keep notebooks up-to-dateWorkflow:
- Primer Design
- Streaking a Plate for Isolation
- Overnight Cultures
- 1) Isolation of Plasmid DNA (miniprep)
- 2) General PCR Protocol
- 3) General Digestion Protocol
- 4) Agarose Gel Electrophoresis
- 5) General Ligation Protocol
- 6) Heat shock/chemical competent transformation
- 7) Colony PCR with Green taq
- 8) DNA Sequencing
- 9) Making Glycerol Frozen Stocks
