Team:Washington/HOMEPAGE PROTOCOL

From 2013.igem.org

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<u>Cloning Protocols Workflow</u>
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Tips and Etiquette:
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<li><a href = "https://2013.igem.org/T<html>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">General Basic Protocol Information</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">General Basic Protocol Information</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">How to Label EVERYTHING</a></li>
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</ul>
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Keep notebooks up-to-date
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</p>
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Workflow:
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<ul>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">Primer Design</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/HOW_TO_LABEL_EVERYTHING">Streaking a Plate for Isolation</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">Overnight Cultures</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">1) Isolation of Plasmid DNA (miniprep)</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">2) General PCR Protocol </a>(also see <a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">PCR GoTag</a> - product (30-200ng/ ul) Check on gel PCR Purification and/or  <a href = "">Dpnl Digest</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">3) General Digestion Protocol</a> Check on gel PCR Purification or Heat Inactivation (check enzyme temp and time, usually 80C for 20min)</li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">4) Agarose Gel Electrophoresis</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">5) General Ligation Protocol</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">6) Heat shock/chemical competent transformation</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">7) Colony PCR with Green taq</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">8) DNA Sequencing</a></li>
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<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">9) Making Glycerol Frozen Stocks</a></li>
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</ul>
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<p1>
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I AM PARAGRPAHIRGJIG 2
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eam:Washington/GENERAL_BASIC_PROTOCOL">General Basic Protocol Information</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">How to Label EVERYTHING</a></li>
<li><a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">How to Label EVERYTHING</a></li>
</ul>
</ul>

Revision as of 01:51, 7 September 2013

Cloning Protocols Workflow

Tips and Etiquette:

Keep notebooks up-to-date

Workflow:


I AM PARAGRPAHIRGJIG 2 eam:Washington/GENERAL_BASIC_PROTOCOL">General Basic Protocol Information</a></li>

  • <a href = "https://2013.igem.org/Team:Washington/GENERAL_BASIC_PROTOCOL">How to Label EVERYTHING</a></li> </ul> Keep notebooks up-to-date </p>

    Workflow:


    <p1> I AM PARAGRPAHIRGJIG 2 </p1>


    </body>

    </html>

    Retrieved from "http://2013.igem.org/Team:Washington/HOMEPAGE_PROTOCOL"