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Team:Washington/HOMEPAGE PROTOCOL
From 2013.igem.org
Tips and Etiquette:
Keep notebooks up-to-dateWorkflow:
- Primer Design
- Streaking a Plate for Isolation
- Overnight Cultures
- 1) Isolation of Plasmid DNA (miniprep)
- 2) General PCR Protocol (also see PCR GoTag - product (30-200ng/ ul)
- 3) General Digestion Protocol
- 4) Agarose Gel Electrophoresis
- 5) General Ligation Protocol
- 6) Heat shock/chemical competent transformation
- 7) Colony PCR with Green taq
- 8) DNA Sequencing
- 9) Making Glycerol Frozen Stocks
