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Microfluidic Experiment Record

2013/9/18 10:50:50--Chun Tang

2013/9/25 20:38:47

8th August Plasmid:Gfp(pSB1C3) aiia(pSB3T5) Bacteria:BL21(DE3) Condition: No flowing medium culture supply. Cells trap in the micro pools. Without antibiotic putting in. Total Obserbation Time:200mins Analysis: At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew as well as stronger fluorescence was detected. From then on, we observed weak oscillation of the fluorescence intensity.

2013/9/18 10:51:01--Chun Tang

2013/9/25 20:42:38

9th August Plasmid:Gfp(pSB1C3) aiia(pSB3T5) Bacteria:BL21(DE3) Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic putting in. Total Obserbation Time:140mins Analysis: At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew but the population is still not large enough to observe convictive fluorescence intensity changing.

2013/9/18 10:51:41--Chun Tang

2013/9/25 20:45:15

25th August Plasmid:Gfp(pSB1C3) aiia(pSB3T5) Bacteria:DH5a Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic putting in. Total Obserbation Time:165mins Analysis: At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew and we detect stronger fluorescence degree. From then on, the fluorescence degree kept in almost the same intensity.

2013/9/18 10:52:00--Chun Tang

2013/9/25 20:46:04

31th Auguest Plasmid:Gfp(pSB1C3) aiia(pSB3T5) Bacteria:DH5a Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. Total Obserbation Time:80mins Analysis: At the beginning, the population of cell was large enough to get valid fluorescence degree. And from then on , we observed no fluorescence intensity changing.

2013/9/18 10:52:31--Chun Tang

2013/9/25 20:47:42

12th September Plasmid:Gfp(pSB1C3) aiia(pSB3T5) Bacteria:DH5a Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. Total Obserbation Time:180mins Analysis: At the beginning, the population of cell was large enough to get valid fluorescence degree. From then on ,there was a weak decrease of the fluorescence degee.

2013/9/18 10:53:02--Chun Tang

2013/9/25 20:50:35

15th September Plasmid:Gfp(pSB1C3) aiia(pSB3T5) Bacteria: BL21(WT) Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. Total Obserbation Time:515mins Analysis: From all the mircrofluid images, there is a tendency that the fluorescence degree change from weak to strong and then change from strong to weak.

2013/9/18 10:53:24--Chun Tang

2013/9/25 20:52:02

18th September Plasmid:sfGfp(coE1) aiia(pSB3T5) Bacteria: BL21(WT) Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. Total Obserbation Time:590mins Analysis: There was a strong tendency that the fluorescence intensity came down, and it's the first time that we detected the most obvious changing.

2013/9/25 21:10:34--Chun Tang

2013/9/25 21:11:06

20th September Plasmid:sfGfp(coE1) aiia(pSB3T5) Bacteria: BL21(WT) Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. Total Obserbation Time:470mins Analysis: From all the microfluid images, there is a very weak decrease of the fluorescence degree.

2013/9/25 21:12:48--Chun Tang

2013/9/25 21:13:01

22th September Plasmid:sfGfp(coE1) aiia(pSB3T5) Bacteria: BL21(WT) Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. Total Obserbation Time:? Analysis: ?

2013/9/25 21:15:00--Chun Tang

2013/9/25 21:15:47	Microscope
2013/9/25 21:16:23	Microfluidic chip
2013/9/25 21:16:51	Valves