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Team:XMU-China/wetlab journal
From 2013.igem.org
(Difference between revisions)
| Line 89: | Line 89: | ||
Plasmid:Gfp(pSB1C3) aiia(pSB3T5) | Plasmid:Gfp(pSB1C3) aiia(pSB3T5) | ||
Bacteria:BL21(DE3) | Bacteria:BL21(DE3) | ||
| - | Condition: | + | Condition: Supply medium culture is not flowing.. Cells trap in the micro pools. Without antibiotic putting in. |
Total Obserbation Time:200mins | Total Obserbation Time:200mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew as well as stronger fluorescence was detected. From then on, we observed weak oscillation of the fluorescence intensity. | At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew as well as stronger fluorescence was detected. From then on, we observed weak oscillation of the fluorescence intensity. | ||
</textarea> <br> </td> </tr> | </textarea> <br> </td> </tr> | ||
| Line 138: | Line 139: | ||
Plasmid:Gfp(pSB1C3) aiia(pSB3T5) | Plasmid:Gfp(pSB1C3) aiia(pSB3T5) | ||
Bacteria:BL21(DE3) | Bacteria:BL21(DE3) | ||
| - | Condition: | + | Condition: Supply medium culture is not flowing.. Cells trap in the micro pools. With antibiotic |
putting in. | putting in. | ||
Total Obserbation Time:140mins | Total Obserbation Time:140mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew but the population is still not large enough to observe convictive fluorescence intensity changing. | At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew but the population is still not large enough to observe convictive fluorescence intensity changing. | ||
</textarea> <br> </td> </tr> | </textarea> <br> </td> </tr> | ||
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Plasmid:Gfp(pSB1C3) aiia(pSB3T5) | Plasmid:Gfp(pSB1C3) aiia(pSB3T5) | ||
Bacteria:DH5a | Bacteria:DH5a | ||
| - | Condition: | + | Condition: Supply medium culture is not flowing.. Cells trap in the micro pools. With antibiotic putting in. |
Total Obserbation Time:165mins | Total Obserbation Time:165mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew and we detect stronger fluorescence degree. From then on, the fluorescence degree kept in almost the same intensity. | At the beginning, the population of cells is small and the fluorescence degree is as the same. Minutes later, more cells grew and we detect stronger fluorescence degree. From then on, the fluorescence degree kept in almost the same intensity. | ||
</textarea> <br> </td> </tr> | </textarea> <br> </td> </tr> | ||
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Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | ||
Total Obserbation Time:80mins | Total Obserbation Time:80mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
At the beginning, the population of cell was large enough to get valid fluorescence degree. | At the beginning, the population of cell was large enough to get valid fluorescence degree. | ||
And from then on , we observed no fluorescence intensity changing. | And from then on , we observed no fluorescence intensity changing. | ||
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Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | ||
Total Obserbation Time:180mins | Total Obserbation Time:180mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
At the beginning, the population of cell was large enough to get valid fluorescence degree. From then on ,there was a weak decrease of the fluorescence degee. | At the beginning, the population of cell was large enough to get valid fluorescence degree. From then on ,there was a weak decrease of the fluorescence degee. | ||
</textarea> <br> </td> </tr> | </textarea> <br> </td> </tr> | ||
| Line 336: | Line 341: | ||
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | ||
Total Obserbation Time:515mins | Total Obserbation Time:515mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
From all the mircrofluid images, there is a tendency that the fluorescence degree change from weak to strong and then change from strong to weak. | From all the mircrofluid images, there is a tendency that the fluorescence degree change from weak to strong and then change from strong to weak. | ||
</textarea> <br> </td> </tr> | </textarea> <br> </td> </tr> | ||
| Line 385: | Line 391: | ||
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in. | ||
Total Obserbation Time:590mins | Total Obserbation Time:590mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
There was a strong tendency that the fluorescence intensity came down, and it's the first | There was a strong tendency that the fluorescence intensity came down, and it's the first | ||
time that we detected the most obvious changing. | time that we detected the most obvious changing. | ||
| Line 434: | Line 441: | ||
antibiotic putting in. | antibiotic putting in. | ||
Total Obserbation Time:470mins | Total Obserbation Time:470mins | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
From all the microfluid images, there is a very weak decrease of the fluorescence degree. | From all the microfluid images, there is a very weak decrease of the fluorescence degree. | ||
</textarea> <br> </td> </tr> | </textarea> <br> </td> </tr> | ||
| Line 482: | Line 490: | ||
antibiotic putting in. | antibiotic putting in. | ||
Total Obserbation Time:? | Total Obserbation Time:? | ||
| - | Analysis: | + | Capturing time lapse:5mins |
| + | Analysis: | ||
? | ? | ||
</textarea> <br> </td> </tr> | </textarea> <br> </td> </tr> | ||
Revision as of 14:33, 26 September 2013
Microfluidic Experiment Record
2013/9/18 10:50:50--Chun Tang
| 2013/9/25 20:38:47 |
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2013/9/18 10:51:01--Chun Tang
| 2013/9/25 20:42:38 |
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2013/9/18 10:51:41--Chun Tang
| 2013/9/25 20:45:15 |
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2013/9/18 10:52:00--Chun Tang
| 2013/9/25 20:46:04 |
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2013/9/18 10:52:31--Chun Tang
| 2013/9/25 20:47:42 |
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2013/9/18 10:53:02--Chun Tang
| 2013/9/25 20:50:35 |
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2013/9/18 10:53:24--Chun Tang
| 2013/9/25 20:52:02 |
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2013/9/25 21:10:34--Chun Tang
| 2013/9/25 21:11:06 |
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2013/9/25 21:12:48--Chun Tang
| 2013/9/25 21:13:01 |
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2013/9/25 21:15:00--Chun Tang
| 2013/9/25 21:15:47 |
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| 2013/9/25 21:16:23 |
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| 2013/9/25 21:16:51 |
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Biobrick Transformation (6.24-6.30)
2013/9/26 20:16:17--Chun Tang
| 2013/9/26 20:16:39 | |||
| 2013/9/26 20:17:26 |
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Plasmids Construction (7.1-7.28, 8.15-9.16)
2013/9/26 20:36:39--Chun Tang
| 2013/9/26 20:36:58 | |||
| 2013/9/26 20:37:10 |
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2013/9/26 20:37:51--Chun Tang
| 2013/9/26 20:38:17 | |||
| 2013/9/26 20:38:31 |
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| 2013/9/26 20:39:10 |
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2013/9/26 20:40:10--Chun Tang
| 2013/9/26 20:40:27 | |||
| 2013/9/26 20:40:37 |
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| 2013/9/26 20:42:21 |
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2013/9/26 20:43:04--Chun Tang
| 2013/9/26 20:43:20 | |||
| 2013/9/26 20:43:33 |
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| 2013/9/26 20:44:08 |
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2013/9/26 20:44:56--Chun Tang
| 2013/9/26 20:45:14 | |||
| 2013/9/26 20:45:33 |
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| 2013/9/26 20:46:05 |
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2013/9/26 20:47:01--Chun Tang
| 2013/9/26 20:47:15 | |||
| 2013/9/26 20:47:24 |
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| 2013/9/26 20:48:03 |
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2013/9/26 20:48:37--Chun Tang
| 2013/9/26 20:48:50 | |||
| 2013/9/26 20:49:00 |
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Transformation (7.29-9.20)
2013/9/26 20:49:56--Chun Tang
| 2013/9/26 20:50:04 | |||
| 2013/9/26 20:50:14 |
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| 2013/9/26 20:51:15 |
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2013/9/26 20:51:34--Chun Tang
| 2013/9/26 20:51:47 | |||
| 2013/9/26 20:52:17 |
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2013/9/26 20:52:47--Chun Tang
| 2013/9/26 20:53:01 | |||
| 2013/9/26 20:53:09 |
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2013/9/26 20:55:54--Chun Tang
| 2013/9/26 20:56:19 |
SDS-PAGE
2013/9/26 21:48:57--Tang Chun
| 2013/9/26 21:49:44 |
2013/9/26 21:50:08--Tang Chun
| 2013/9/26 21:51:07 |
2013/9/26 21:53:17--Tang Chun
| 2013/9/26 21:55:45 |
2013/9/26 22:02:08--Tang Chun
| 2013/9/26 22:03:34 |
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2013/9/26 22:04:18--Tang Chun
| 2013/9/26 22:07:07 |
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2013/9/26 22:07:33--Tang Chun
| 2013/9/26 22:08:04 | |||
| 2013/9/26 22:07:46 |
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2013/9/26 22:08:31--Tang Chun
| 2013/9/26 22:08:47 |
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2013/9/26 22:09:10--Tang Chun
| 2013/9/26 22:09:28 |
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| 2013/9/26 22:09:40 |
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2013/9/26 22:10:13--Tang Chun
| 2013/9/26 22:10:54 |
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| 2013/9/26 22:11:28 |
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2013/9/26 22:12:08--Tang Chun
| 2013/9/26 22:12:23 |
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| 2013/9/26 22:12:32 |
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2013/9/26 22:13:05--Tang Chun
| 2013/9/26 22:13:16 |
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