From 2013.igem.org
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| | Lane2,5,8: GFP aiiA ndh, digestion of EcoRI | | Lane2,5,8: GFP aiiA ndh, digestion of EcoRI |
| | Lane3,4,9: GFP aiiA ndh plasmids | | Lane3,4,9: GFP aiiA ndh plasmids |
| - | </textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="BL21 (WT) (9.11-9.20)" id="sb6s2"> <button class="M1_exp_step_name_button"><input value="BL21 (WT) (9.11-9.20)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:52:47--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s2i0">2013/9/26 20:53:01</td> <td class="M1_exp_item_content"><textarea id="i6s2i0" class="C0" rows="1"> Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed aiiA/ndh pSB3T5, sfGFP, colE1 GFP, colE1 sfGFP and GFP pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s2i1">2013/9/28 01:24:59</td> <td class="M1_exp_item_content"> <table id="i6s2i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s2i10" src="http://trysomething-block.stor.sinaapp.com/t14i6s2i2图片17.jpg"><br> <form id="i6s2i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s2i12" form="i6s2i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s2i14"> <input name="id" type="hidden" value="i6s2i1" form="i6s2i14"> <input name="oldgraph" type="hidden" id="i6s2i15" form="i6s2i14" value="t14i6s2i2图片17.jpg"> <input id="i6s2i16" type="submit" value="submit" form="i6s2i14" class="C1_button2" disabled=""> <div id="i6s2i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s2i11" class="C1_text">M:Marker 1kb | + | </textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="BL21 (WT) (9.11-9.20)" id="sb6s2"> <button class="M1_exp_step_name_button"><input value="BL21 (WT) (9.11-9.20)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:52:47--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s2i0">2013/9/26 20:53:01</td> <td class="M1_exp_item_content"><textarea id="i6s2i0" class="C0" rows="1"> Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed aiiA/ndh pSB3T5, sfGFP, colE1 GFP, colE1 sfGFP and GFP pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s2i1">2013/9/28 01:24:59</td> <td class="M1_exp_item_content"> <table id="i6s2i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s2i10 " width="450px" height="auto" src="http://trysomething-block.stor.sinaapp.com/t14i6s2i2图片17.jpg"><br> <form id="i6s2i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s2i12" form="i6s2i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s2i14"> <input name="id" type="hidden" value="i6s2i1" form="i6s2i14"> <input name="oldgraph" type="hidden" id="i6s2i15" form="i6s2i14" value="t14i6s2i2图片17.jpg"> <input id="i6s2i16" type="submit" value="submit" form="i6s2i14" class="C1_button2" disabled=""> <div id="i6s2i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s2i11" class="C1_text">M:Marker 1kb |
| | Lane1,4 :GFP(1A3) aiiA ndh plasmid | | Lane1,4 :GFP(1A3) aiiA ndh plasmid |
| | Lane2,5 : GFP(1A3) aiiA ndh plasmid,digestion of XbaI | | Lane2,5 : GFP(1A3) aiiA ndh plasmid,digestion of XbaI |
Revision as of 01:45, 28 September 2013
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