From 2013.igem.org
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| | <div class="microfluidic"> | | <div class="microfluidic"> |
| | <h1>Transformation (7.29-9.20)</h1> | | <h1>Transformation (7.29-9.20)</h1> |
| - | <div class="M1_exp_stepInfo" title="BL21 (DE3) (7.29-8.15)" id="sb6s0"> <button class="M1_exp_step_name_button"><input value="BL21 (DE3) (7.29-8.15)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:49:56--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:50:04</td> <td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1"> Because we had constructed three plasmids we need, our next goal was to transform the plasmids into BL21(DE3). We first constructed a double-plasmid BL21: GFP aiiA, and then we transformed the third plasmid into BL21 with two plasmids.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i1">2013/9/28 01:21:56</td> <td class="M1_exp_item_content"> <table id="i6s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s0i10" src="http://trysomething-block.stor.sinaapp.com/t14i6s0i3图片14.jpg"><br> <form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s0i14"> <input name="id" type="hidden" value="i6s0i1" form="i6s0i14"> <input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i3图片14.jpg"> <input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled=""> <div id="i6s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s0i11" class="C1_text">M:Marker 1kb | + | <div class="M1_exp_stepInfo" title="BL21 (DE3) (7.29-8.15)" id="sb6s0"> <button class="M1_exp_step_name_button"><input value="BL21 (DE3) (7.29-8.15)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:49:56--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:50:04</td> <td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1"> Because we had constructed three plasmids we need, our next goal was to transform the plasmids into BL21(DE3). We first constructed a double-plasmid BL21: GFP aiiA, and then we transformed the third plasmid into BL21 with two plasmids.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i1">2013/9/28 01:21:56</td> <td class="M1_exp_item_content"> <table id="i6s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s0i10" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i6s0i3图片14.jpg"><br> <form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s0i14"> <input name="id" type="hidden" value="i6s0i1" form="i6s0i14"> <input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i3图片14.jpg"> <input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled=""> <div id="i6s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s0i11" class="C1_text">M:Marker 1kb |
| | Lane1:GFP plasmid,digestion of EcoRI and SpeI | | Lane1:GFP plasmid,digestion of EcoRI and SpeI |
| | Lane2:GFP plasmid,digestion of EcoRI | | Lane2:GFP plasmid,digestion of EcoRI |
Revision as of 01:47, 28 September 2013
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