The band marked in red was not the target band. Maybe it is the complex of AHL-LuxI. The target proteins of pSB1C3-gfp-luxI have been expressed while the target proteins of pSB3T5-aiiA and pSB4K5-ndh did not be expressed because the promoter should be induced by AHL.
The band marked in red was not the target band. Maybe it is the complex of AHL-LuxI. The target proteins of pSB1C3-gfp-luxI have been expressed while the target proteins of pSB3T5-aiiA and pSB4K5-ndh did not be expressed because the promoter should be induced by AHL.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" style="background-color: rgb(166, 166, 166);"> <button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td> <td class="M1_exp_item_content"> <table id="i7s7i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br> <form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i04"> <input name="id" type="hidden" value="i7s7i0" form="i7s7i04"> <input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png"> <input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;"> We were suggested that DH5α has a better viability and a higher protein expression. So we maybe there's some mistakes in our strains which cause our couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" style="background-color: rgb(166, 166, 166);"> <button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td> <td class="M1_exp_item_content"> <table id="i7s7i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br> <form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i04"> <input name="id" type="hidden" value="i7s7i0" form="i7s7i04"> <input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png"> <input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;"> We were suggested that DH5α has a better viability and a higher protein expression. So we maybe there's some mistakes in our strains which cause our couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
Data analysis:
Data analysis:
E.coli maybe cultured at 30℃ had a stronger expression of protein from picture 1 but we were confused by picture 2 cause it indicated that expression at 30℃ is not larger than expression at 37℃.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i1">2013/9/26 22:09:40</td> <td class="M1_exp_item_content"> <table id="i7s7i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i16.png"><br> <form id="i7s7i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i12" form="i7s7i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i14"> <input name="id" type="hidden" value="i7s7i1" form="i7s7i14"> <input name="oldgraph" type="hidden" id="i7s7i15" form="i7s7i14" value="t14i9s7i16.png"> <input id="i7s7i16" type="submit" value="submit" form="i7s7i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i11" class="C1_text" disabled="" style="background-color: white;">Future plan:
E.coli maybe cultured at 30℃ had a stronger expression of protein from picture 1 but we were confused by picture 2 cause it indicated that expression at 30℃ is not larger than expression at 37℃.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i1">2013/9/26 22:09:40</td> <td class="M1_exp_item_content"> <table id="i7s7i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i16.png"><br> <form id="i7s7i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i12" form="i7s7i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i14"> <input name="id" type="hidden" value="i7s7i1" form="i7s7i14"> <input name="oldgraph" type="hidden" id="i7s7i15" form="i7s7i14" value="t14i9s7i16.png"> <input id="i7s7i16" type="submit" value="submit" form="i7s7i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i11" class="C1_text" disabled="" style="background-color: white;">Future plan:
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Line 206:
There is a paradox between A A and A:
There is a paradox between A A and A:
E.coli cultured has a stronger expression of protein when not added AHL？
E.coli cultured has a stronger expression of protein when not added AHL？
We characterize our biobricks using additional AHL. We bought commercial AHL powder and prepared it into solution. The AHL added into these cells was 1000 nmol/L and BL21 was wild type.
We characterize our biobricks using additional AHL. We bought commercial AHL powder and prepared it into solution. The AHL added into these cells was 1000 nmol/L and BL21 was wild type.
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