From 2013.igem.org

(Difference between revisions)

Revision as of 02:00, 28 September 2013

导航栏

Biobrick Transformation (6.24-6.30)

2013/9/26 20:16:17--Chun Tang

2013/9/26 20:16:39

2013/9/26 20:17:26

Plasmids Construction (7.1-7.28, 8.15-9.16)

2013/9/26 20:36:39--Chun Tang

2013/9/26 20:36:58

2013/9/28 01:03:53

2013/9/26 20:37:51--Chun Tang

2013/9/26 20:38:17

2013/9/28 01:05:10

2013/9/28 01:05:15

2013/9/26 20:40:10--Chun Tang

2013/9/26 20:40:27

2013/9/28 01:06:41

2013/9/28 01:06:51

2013/9/26 20:43:04--Chun Tang

2013/9/26 20:43:20

2013/9/28 01:10:10

2013/9/28 01:10:38

2013/9/26 20:44:56--Chun Tang

2013/9/26 20:45:14

2013/9/28 01:11:54

2013/9/28 01:12:42

2013/9/26 20:47:01--Chun Tang

2013/9/26 20:47:15

2013/9/28 01:16:48

2013/9/28 01:17:27

2013/9/26 20:48:37--Chun Tang

2013/9/26 20:48:50

2013/9/28 01:19:02

Transformation (7.29-9.20)

2013/9/26 20:49:56--Chun Tang

2013/9/26 20:50:04

2013/9/28 01:21:56

2013/9/28 01:22:37

2013/9/26 20:51:34--Chun Tang

2013/9/26 20:51:47

2013/9/28 01:23:53

2013/9/26 20:52:47--Chun Tang

2013/9/26 20:53:01

2013/9/28 01:24:59

2013/9/26 20:55:54--Chun Tang

2013/9/26 20:56:19

SDS-PAGE

2013/9/26 21:48:57--Tang Chun

2013/9/26 21:49:44

2013/9/26 21:50:08--Tang Chun

2013/9/26 21:51:07

2013/9/26 21:53:17--Tang Chun

2013/9/26 21:55:45

2013/9/26 22:02:08--Tang Chun

2013/9/26 22:03:34

2013/9/26 22:04:18--Tang Chun

2013/9/26 22:07:07

2013/9/26 22:07:33--Tang Chun

2013/9/26 22:08:04

2013/9/26 22:07:46

2013/9/26 22:08:31--Tang Chun

2013/9/26 22:08:47

2013/9/26 22:09:10--Tang Chun

2013/9/26 22:09:28

2013/9/26 22:09:40

2013/9/26 22:10:13--Tang Chun

2013/9/26 22:10:54

2013/9/26 22:11:28

2013/9/26 22:12:08--Tang Chun

2013/9/26 22:12:23

2013/9/26 22:12:32

2013/9/26 22:13:05--Tang Chun

2013/9/26 22:13:16

2013/9/28 07:03:20--Chun Tang

2013/9/28 07:03:54

2013/9/28 08:44:02--Chun Tang

2013/9/28 08:44:22

2013/9/28 08:47:11

2013/9/28 08:44:24

2013/9/28 08:44:28

Microfluidic Experiment Record

2013/9/18 10:50:50--Chun Tang

2013/9/25 20:38:47

2013/9/18 10:51:01--Chun Tang

2013/9/25 20:42:38

2013/9/18 10:51:41--Chun Tang

2013/9/25 20:45:15

2013/9/18 10:52:00--Chun Tang

2013/9/25 20:46:04

2013/9/18 10:52:31--Chun Tang

2013/9/25 20:47:42

2013/9/18 10:53:02--Chun Tang

2013/9/25 20:50:35

2013/9/18 10:53:24--Chun Tang

2013/9/25 20:52:02

2013/9/25 21:10:34--Chun Tang

2013/9/25 21:11:06

2013/9/25 21:12:48--Chun Tang

2013/9/25 21:13:01

2013/9/28 07:14:25

2013/9/28 07:14:27

2013/9/28 07:14:28

2013/9/25 21:15:00--Chun Tang

2013/9/25 21:15:47

2013/9/25 21:16:23

2013/9/25 21:16:51

RFU (9.24-9.27)

2013/9/27 21:59:07--Chun Tang

2013/9/27 21:59:17

2013/9/28 03:11:44

2013/9/28 03:30:57

2013/9/28 03:31:38

2013/9/28 03:34:06

@@ Line 166: / Line 166: @@
 <div class="microfluidic">
 <h1>SDS-PAGE</h1>
-				<div class="M1_exp_stepInfo" title="Abbreviation:" id="sb7s0" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="Abbreviation:" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 21:48:57--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s0i0">2013/9/26 21:49:44</td>			<td class="M1_exp_item_content"><textarea id="i7s0i0" class="C0" rows="1" disabled="" style="background-color: white;">W: Whole cell   P: Precipitation   S: Supernatant
+				<div class="M1_exp_stepInfo" title="Abbreviation:" id="sb7s0" >			<button class="M1_exp_step_name_button" ><input value="Abbreviation:" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 21:48:57--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s0i0">2013/9/26 21:49:44</td>			<td class="M1_exp_item_content"><textarea id="i7s0i0" class="C0" rows="1" disabled="" style="background-color: white;">W: Whole cell   P: Precipitation   S: Supernatant
-</textarea></td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="Background" id="sb7s1" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="Background" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 21:50:08--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s1i0">2013/9/26 21:51:07</td>			<td class="M1_exp_item_content"><table id="i7s1i0" class="C3"><tbody><tr><td class="C3_td"><input value="CDS" class="C3_input"></td><td class="C3_td"><input value="Size(bp)" class="C3_input"></td><td class="C3_td"><input value="Protein" class="C3_input"></td><td class="C3_td"><input value="Size(kDa)" class="C3_input"></td></tr><tr><td class="C3_td"><input value="luxR" class="C3_input"></td><td class="C3_td"><input value="750" class="C3_input"></td><td class="C3_td"><input value="LuxR" class="C3_input"></td><td class="C3_td"><input value="27.5" class="C3_input"></td></tr><tr><td class="C3_td"><input value="gfp-lva" class="C3_input"></td><td class="C3_td"><input value="753" class="C3_input"></td><td class="C3_td"><input value="GFP-LVA" class="C3_input"></td><td class="C3_td"><input value="27.61" class="C3_input"></td></tr><tr><td class="C3_td"><input value="aiiA-lva" class="C3_input"></td><td class="C3_td"><input value="783" class="C3_input"></td><td class="C3_td"><input value="aiiA-LVA" class="C3_input"></td><td class="C3_td"><input value="28.71" class="C3_input"></td></tr><tr><td class="C3_td"><input value="luxI-lva" class="C3_input"></td><td class="C3_td"><input value="612" class="C3_input"></td><td class="C3_td"><input value="LuxI-LVA" class="C3_input"></td><td class="C3_td"><input value="22.44" class="C3_input"></td></tr><tr><td class="C3_td"><input value="ndh-lva" class="C3_input"></td><td class="C3_td"><input value="1335" class="C3_input"></td><td class="C3_td"><input value="NDH-LVA" class="C3_input"></td><td class="C3_td"><input value="48.95" class="C3_input"></td></tr></tbody></table></td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="Graphic Symbol" id="sb7s2" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="Graphic Symbol" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 21:53:17--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s2i0">2013/9/26 21:55:45</td>			<td class="M1_exp_item_content"><table id="i7s2i0" class="C3"><tbody><tr><td class="C3_td"><input value="Strain" class="C3_input"></td><td class="C3_td"><input value="Plasmids" class="C3_input"></td><td class="C3_td"><input value="Proteins" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td><td class="C3_td"><input value="NDH" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp-luxI" class="C3_input"></td><td class="C3_td"><input value="Luxr,LuxI-LVA,GFP-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-B" class="C3_input"></td><td class="C3_td"><input value="pSB3T5-aiiA" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-C" class="C3_input"></td><td class="C3_td"><input value="pSB4K5-ndh" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A B" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp-luxI pSB3T5-aiiA" class="C3_input"></td><td class="C3_td"><input value="Luxr,LuxI-LVA,GFP,aiiA-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A B C" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp luxI pSB3T5-aiiA pSB4K5-ndh" class="C3_input"></td><td class="C3_td"><input value="LuxR,LuxI-LVA,GFP-LVA.aiiA-LVA.NDH-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="" class="C3_input"></td><td class="C3_td"><input value="" class="C3_input"></td><td class="C3_td"><input value="" class="C3_input"></td></tr></tbody></table></td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="1. Characterization of expression of three single plasmids in BL21. (8.02-8. 08)" id="sb7s3" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="1. Characterization of expression of three single plasmids in BL21. (8.02-8. 08)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:02:08--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s3i0">2013/9/26 22:03:34</td>			<td class="M1_exp_item_content">	<table id="i7s3i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s3i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s3i01.png"><br>				<form id="i7s3i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s3i02" form="i7s3i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s3i04">				<input name="id" type="hidden" value="i7s3i0" form="i7s3i04">				<input name="oldgraph" type="hidden" id="i7s3i05" form="i7s3i04" value="t14i9s3i01.png">				<input id="i7s3i06" type="submit" value="submit" form="i7s3i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s3i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s3i01" class="C1_text" disabled="" style="background-color: white;">  We compared protein bands between BL21, the blank control and three groups transformed into different single plasmids, aiiA, ndh and gfp. These two gel pictures were got in two different days.
+</textarea></td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="Background" id="sb7s1" >			<button class="M1_exp_step_name_button" ><input value="Background" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 21:50:08--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s1i0">2013/9/26 21:51:07</td>			<td class="M1_exp_item_content"><table id="i7s1i0" class="C3"><tbody><tr><td class="C3_td"><input value="CDS" class="C3_input"></td><td class="C3_td"><input value="Size(bp)" class="C3_input"></td><td class="C3_td"><input value="Protein" class="C3_input"></td><td class="C3_td"><input value="Size(kDa)" class="C3_input"></td></tr><tr><td class="C3_td"><input value="luxR" class="C3_input"></td><td class="C3_td"><input value="750" class="C3_input"></td><td class="C3_td"><input value="LuxR" class="C3_input"></td><td class="C3_td"><input value="27.5" class="C3_input"></td></tr><tr><td class="C3_td"><input value="gfp-lva" class="C3_input"></td><td class="C3_td"><input value="753" class="C3_input"></td><td class="C3_td"><input value="GFP-LVA" class="C3_input"></td><td class="C3_td"><input value="27.61" class="C3_input"></td></tr><tr><td class="C3_td"><input value="aiiA-lva" class="C3_input"></td><td class="C3_td"><input value="783" class="C3_input"></td><td class="C3_td"><input value="aiiA-LVA" class="C3_input"></td><td class="C3_td"><input value="28.71" class="C3_input"></td></tr><tr><td class="C3_td"><input value="luxI-lva" class="C3_input"></td><td class="C3_td"><input value="612" class="C3_input"></td><td class="C3_td"><input value="LuxI-LVA" class="C3_input"></td><td class="C3_td"><input value="22.44" class="C3_input"></td></tr><tr><td class="C3_td"><input value="ndh-lva" class="C3_input"></td><td class="C3_td"><input value="1335" class="C3_input"></td><td class="C3_td"><input value="NDH-LVA" class="C3_input"></td><td class="C3_td"><input value="48.95" class="C3_input"></td></tr></tbody></table></td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="Graphic Symbol" id="sb7s2" >			<button class="M1_exp_step_name_button" ><input value="Graphic Symbol" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 21:53:17--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s2i0">2013/9/26 21:55:45</td>			<td class="M1_exp_item_content"><table id="i7s2i0" class="C3"><tbody><tr><td class="C3_td"><input value="Strain" class="C3_input"></td><td class="C3_td"><input value="Plasmids" class="C3_input"></td><td class="C3_td"><input value="Proteins" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td><td class="C3_td"><input value="NDH" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp-luxI" class="C3_input"></td><td class="C3_td"><input value="Luxr,LuxI-LVA,GFP-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-B" class="C3_input"></td><td class="C3_td"><input value="pSB3T5-aiiA" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-C" class="C3_input"></td><td class="C3_td"><input value="pSB4K5-ndh" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A B" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp-luxI pSB3T5-aiiA" class="C3_input"></td><td class="C3_td"><input value="Luxr,LuxI-LVA,GFP,aiiA-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A B C" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp luxI pSB3T5-aiiA pSB4K5-ndh" class="C3_input"></td><td class="C3_td"><input value="LuxR,LuxI-LVA,GFP-LVA.aiiA-LVA.NDH-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="" class="C3_input"></td><td class="C3_td"><input value="" class="C3_input"></td><td class="C3_td"><input value="" class="C3_input"></td></tr></tbody></table></td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="1. Characterization of expression of three single plasmids in BL21. (8.02-8. 08)" id="sb7s3" >			<button class="M1_exp_step_name_button" ><input value="1. Characterization of expression of three single plasmids in BL21. (8.02-8. 08)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:02:08--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s3i0">2013/9/26 22:03:34</td>			<td class="M1_exp_item_content">	<table id="i7s3i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s3i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s3i01.png"><br>				<form id="i7s3i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s3i02" form="i7s3i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s3i04">				<input name="id" type="hidden" value="i7s3i0" form="i7s3i04">				<input name="oldgraph" type="hidden" id="i7s3i05" form="i7s3i04" value="t14i9s3i01.png">				<input id="i7s3i06" type="submit" value="submit" form="i7s3i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s3i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s3i01" class="C1_text" disabled="" style="background-color: white;">  We compared protein bands between BL21, the blank control and three groups transformed into different single plasmids, aiiA, ndh and gfp. These two gel pictures were got in two different days.
 Data analysis:
-   The target proteins of pSB3T5-aiiA/BL21 and pSB4K5-ndh/BL21 should not be expressed because the promoter should be induced by AHL. The target proteins of pSB1C3-gfp-luxI/BL21 have been expressed but GFP was misfolded we suppose. However, the band marked in red was not the target band. Maybe it's the complex of AHL-LuxI.</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="2. Characterization of expression of double plasmids (8.05)" id="sb7s4" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="2. Characterization of expression of double plasmids (8.05)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:04:18--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s4i0">2013/9/26 22:07:07</td>			<td class="M1_exp_item_content">	<table id="i7s4i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s4i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s4i02.png"><br>				<form id="i7s4i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s4i02" form="i7s4i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s4i04">				<input name="id" type="hidden" value="i7s4i0" form="i7s4i04">				<input name="oldgraph" type="hidden" id="i7s4i05" form="i7s4i04" value="t14i9s4i02.png">				<input id="i7s4i06" type="submit" value="submit" form="i7s4i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s4i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s4i01" class="C1_text" disabled="" style="background-color: white;">  We performed a SDS-PAGE comparing expression of double plasmids, gfp and aiiA, and two corresponding single plasmids. What's more, we wanted to find more details about band which possibly is the misfolded gfp.
+   The target proteins of pSB3T5-aiiA/BL21 and pSB4K5-ndh/BL21 should not be expressed because the promoter should be induced by AHL. The target proteins of pSB1C3-gfp-luxI/BL21 have been expressed but GFP was misfolded we suppose. However, the band marked in red was not the target band. Maybe it's the complex of AHL-LuxI.</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="2. Characterization of expression of double plasmids (8.05)" id="sb7s4" >			<button class="M1_exp_step_name_button" ><input value="2. Characterization of expression of double plasmids (8.05)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:04:18--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s4i0">2013/9/26 22:07:07</td>			<td class="M1_exp_item_content">	<table id="i7s4i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s4i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s4i02.png"><br>				<form id="i7s4i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s4i02" form="i7s4i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s4i04">				<input name="id" type="hidden" value="i7s4i0" form="i7s4i04">				<input name="oldgraph" type="hidden" id="i7s4i05" form="i7s4i04" value="t14i9s4i02.png">				<input id="i7s4i06" type="submit" value="submit" form="i7s4i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s4i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s4i01" class="C1_text" disabled="" style="background-color: white;">  We performed a SDS-PAGE comparing expression of double plasmids, gfp and aiiA, and two corresponding single plasmids. What's more, we wanted to find more details about band which possibly is the misfolded gfp.
 Data analysis:
    The band marked in red disappeared is pSB1C3-gfp-luxI/BL21.The LuxR, GFP and AiiA  are the same of size (marked in yellow). However the band of AiiA was not clear. Many target proteins were misfolded. Therefore the culture condition should be optimized.
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="3. (8.12)" id="sb7s5" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="3. (8.12)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:07:33--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i0">2013/9/26 22:08:04</td>			<td class="M1_exp_item_content"><textarea id="i7s5i0" class="C0" rows="1" disabled="" style="background-color: white;"> Characterization of expression of E.coli transformed into triple plasmids and testing if our target proteins expressed by our constructed gene circuits were concentrated in precipitation or supernatant  (8.12)</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i1">2013/9/26 22:07:46</td>			<td class="M1_exp_item_content">	<table id="i7s5i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s5i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s5i03.png"><br>				<form id="i7s5i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s5i12" form="i7s5i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s5i14">				<input name="id" type="hidden" value="i7s5i1" form="i7s5i14">				<input name="oldgraph" type="hidden" id="i7s5i15" form="i7s5i14" value="t14i9s5i03.png">				<input id="i7s5i16" type="submit" value="submit" form="i7s5i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s5i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s5i11" class="C1_text" disabled="" style="background-color: white;">Description :
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="3. (8.12)" id="sb7s5" >			<button class="M1_exp_step_name_button" ><input value="3. (8.12)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:07:33--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i0">2013/9/26 22:08:04</td>			<td class="M1_exp_item_content"><textarea id="i7s5i0" class="C0" rows="1" disabled="" style="background-color: white;"> Characterization of expression of E.coli transformed into triple plasmids and testing if our target proteins expressed by our constructed gene circuits were concentrated in precipitation or supernatant  (8.12)</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i1">2013/9/26 22:07:46</td>			<td class="M1_exp_item_content">	<table id="i7s5i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s5i10" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s5i03.png"><br>				<form id="i7s5i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s5i12" form="i7s5i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s5i14">				<input name="id" type="hidden" value="i7s5i1" form="i7s5i14">				<input name="oldgraph" type="hidden" id="i7s5i15" form="i7s5i14" value="t14i9s5i03.png">				<input id="i7s5i16" type="submit" value="submit" form="i7s5i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s5i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s5i11" class="C1_text" disabled="" style="background-color: white;">Description :
   We had transformed triple plasmids into one E.coli and the DNA gel picture indicated it was ligated successfully. So now we were very eager to know if our circuits could work well in E.coli.
 Furthermore, we wanted to know that if our target proteins expressed by our constructed gene circuits, in other words, pSB1C3-gfp-luxI and pSB13T5-aiiA were aggregated in precipitation or supernatant. So we run SDS-PAGE using BL21(blank control), three cells transformed into different single plasmids(aiiA, ndh and gfp), one cell transformed into double plasmids(aiiA and gfp) and our E.coli transformed into triple plasmids in three forms respectively, whole cell, precipitation and supernatant.
@@ Line 178: / Line 178: @@
 Data analysis:
    From the SDS-PAGE gel pictures, we could easily find that the clear AiiA band (marked in red) but NDH was not clear in PAGE because the gene ndh did exist in the genome DNA of BL21 native strain.
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="4. Characterization of expression of single plasmid in DH5α(8.17)" id="sb7s6" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="4. Characterization of expression of single plasmid in DH5α(8.17)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:08:31--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s6i0">2013/9/26 22:08:47</td>			<td class="M1_exp_item_content">	<table id="i7s6i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s6i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s6i04.png"><br>				<form id="i7s6i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s6i02" form="i7s6i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s6i04">				<input name="id" type="hidden" value="i7s6i0" form="i7s6i04">				<input name="oldgraph" type="hidden" id="i7s6i05" form="i7s6i04" value="t14i9s6i04.png">				<input id="i7s6i06" type="submit" value="submit" form="i7s6i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s6i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s6i01" class="C1_text" disabled="" style="background-color: white;">  We've found that maybe there's some mistakes in our strains which cause us couldn't get a expected microfluidic experiment result. And we analyzed that DH5α maybe a better strain than BL21 so we changed the host. We firstly confirm the expression of three single plasmids transformed into DH5α.
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="4. Characterization of expression of single plasmid in DH5α(8.17)" id="sb7s6" >			<button class="M1_exp_step_name_button" ><input value="4. Characterization of expression of single plasmid in DH5α(8.17)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:08:31--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s6i0">2013/9/26 22:08:47</td>			<td class="M1_exp_item_content">	<table id="i7s6i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s6i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s6i04.png"><br>				<form id="i7s6i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s6i02" form="i7s6i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s6i04">				<input name="id" type="hidden" value="i7s6i0" form="i7s6i04">				<input name="oldgraph" type="hidden" id="i7s6i05" form="i7s6i04" value="t14i9s6i04.png">				<input id="i7s6i06" type="submit" value="submit" form="i7s6i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s6i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s6i01" class="C1_text" disabled="" style="background-color: white;">  We've found that maybe there's some mistakes in our strains which cause us couldn't get a expected microfluidic experiment result. And we analyzed that DH5α maybe a better strain than BL21 so we changed the host. We firstly confirm the expression of three single plasmids transformed into DH5α.
 Data analysis:
 The band marked in red was not the target band. Maybe it is the complex of AHL-LuxI. The target proteins of pSB1C3-gfp-luxI have been expressed while the target proteins of pSB3T5-aiiA and pSB4K5-ndh did not be  expressed because the promoter should be induced by AHL.
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td>			<td class="M1_exp_item_content">	<table id="i7s7i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br>				<form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s7i04">				<input name="id" type="hidden" value="i7s7i0" form="i7s7i04">				<input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png">				<input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s7i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;">  We were suggested that DH5α has a better viability and a higher protein expression. So we maybe there's some mistakes in our strains which cause our couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" >			<button class="M1_exp_step_name_button" ><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td>			<td class="M1_exp_item_content">	<table id="i7s7i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br>				<form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s7i04">				<input name="id" type="hidden" value="i7s7i0" form="i7s7i04">				<input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png">				<input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s7i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;">  We were suggested that DH5α has a better viability and a higher protein expression. So we maybe there's some mistakes in our strains which cause our couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
 Data analysis:
 E.coli maybe cultured at 30℃ had a stronger expression of protein from picture 1 but we were confused by picture 2 cause it indicated that expression at 30℃ is not larger than expression at 37℃.</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s7i1">2013/9/26 22:09:40</td>			<td class="M1_exp_item_content">	<table id="i7s7i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s7i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i16.png"><br>				<form id="i7s7i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s7i12" form="i7s7i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s7i14">				<input name="id" type="hidden" value="i7s7i1" form="i7s7i14">				<input name="oldgraph" type="hidden" id="i7s7i15" form="i7s7i14" value="t14i9s7i16.png">				<input id="i7s7i16" type="submit" value="submit" form="i7s7i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s7i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s7i11" class="C1_text" disabled="" style="background-color: white;">Future plan:
@@ Line 190: / Line 190: @@
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="6. Repeat SDS-PAGE with optimized conditions.(9.02)" id="sb7s8" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="6. Repeat SDS-PAGE with optimized conditions.(9.02)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:10:13--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s8i0">2013/9/26 22:10:54</td>			<td class="M1_exp_item_content">	<table id="i7s8i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s8i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s8i07.png"><br>				<form id="i7s8i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s8i02" form="i7s8i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s8i04">				<input name="id" type="hidden" value="i7s8i0" form="i7s8i04">				<input name="oldgraph" type="hidden" id="i7s8i05" form="i7s8i04" value="t14i9s8i07.png">				<input id="i7s8i06" type="submit" value="submit" form="i7s8i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s8i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s8i01" class="C1_text" disabled="" style="background-color: white;"></textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s8i1">2013/9/26 22:11:28</td>			<td class="M1_exp_item_content">	<table id="i7s8i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s8i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s8i18.png"><br>				<form id="i7s8i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s8i12" form="i7s8i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s8i14">				<input name="id" type="hidden" value="i7s8i1" form="i7s8i14">				<input name="oldgraph" type="hidden" id="i7s8i15" form="i7s8i14" value="t14i9s8i18.png">				<input id="i7s8i16" type="submit" value="submit" form="i7s8i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s8i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s8i11" class="C1_text" disabled="" style="background-color: white;">Description:
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="6. Repeat SDS-PAGE with optimized conditions.(9.02)" id="sb7s8" >			<button class="M1_exp_step_name_button" ><input value="6. Repeat SDS-PAGE with optimized conditions.(9.02)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:10:13--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s8i0">2013/9/26 22:10:54</td>			<td class="M1_exp_item_content">	<table id="i7s8i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s8i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s8i07.png"><br>				<form id="i7s8i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s8i02" form="i7s8i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s8i04">				<input name="id" type="hidden" value="i7s8i0" form="i7s8i04">				<input name="oldgraph" type="hidden" id="i7s8i05" form="i7s8i04" value="t14i9s8i07.png">				<input id="i7s8i06" type="submit" value="submit" form="i7s8i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s8i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s8i01" class="C1_text" disabled="" style="background-color: white;"></textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s8i1">2013/9/26 22:11:28</td>			<td class="M1_exp_item_content">	<table id="i7s8i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s8i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s8i18.png"><br>				<form id="i7s8i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s8i12" form="i7s8i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s8i14">				<input name="id" type="hidden" value="i7s8i1" form="i7s8i14">				<input name="oldgraph" type="hidden" id="i7s8i15" form="i7s8i14" value="t14i9s8i18.png">				<input id="i7s8i16" type="submit" value="submit" form="i7s8i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s8i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s8i11" class="C1_text" disabled="" style="background-color: white;">Description:
    We changed high pressure homogenizer to get cell fragments, in order to gain a more definite gel analysis. The high pressure homogenizer is under 37.5 kpSI  which supposed much too high to make protein. Even the super protein is far less compared with gel picture made after ultrasonic fragmentation, the unclear band is still there.
 Data analysis:
 The band marked in red was not the target band. The band marked in green was not the target band.
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="7. Repeat SDS-PAGE with optimized conditions.(9.03)" id="sb7s9" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="7. Repeat SDS-PAGE with optimized conditions.(9.03)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:12:08--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s9i0">2013/9/26 22:12:23</td>			<td class="M1_exp_item_content">	<table id="i7s9i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s9i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s9i09.png"><br>				<form id="i7s9i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s9i02" form="i7s9i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s9i04">				<input name="id" type="hidden" value="i7s9i0" form="i7s9i04">				<input name="oldgraph" type="hidden" id="i7s9i05" form="i7s9i04" value="t14i9s9i09.png">				<input id="i7s9i06" type="submit" value="submit" form="i7s9i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s9i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s9i01" class="C1_text" disabled="" style="background-color: white;"></textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s9i1">2013/9/26 22:12:32</td>			<td class="M1_exp_item_content">	<table id="i7s9i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s9i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s9i110.png"><br>				<form id="i7s9i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s9i12" form="i7s9i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s9i14">				<input name="id" type="hidden" value="i7s9i1" form="i7s9i14">				<input name="oldgraph" type="hidden" id="i7s9i15" form="i7s9i14" value="t14i9s9i110.png">				<input id="i7s9i16" type="submit" value="submit" form="i7s9i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s9i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s9i11" class="C1_text" disabled="" style="background-color: white;">Description:
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="7. Repeat SDS-PAGE with optimized conditions.(9.03)" id="sb7s9" >			<button class="M1_exp_step_name_button" ><input value="7. Repeat SDS-PAGE with optimized conditions.(9.03)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:12:08--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s9i0">2013/9/26 22:12:23</td>			<td class="M1_exp_item_content">	<table id="i7s9i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s9i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s9i09.png"><br>				<form id="i7s9i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s9i02" form="i7s9i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s9i04">				<input name="id" type="hidden" value="i7s9i0" form="i7s9i04">				<input name="oldgraph" type="hidden" id="i7s9i05" form="i7s9i04" value="t14i9s9i09.png">				<input id="i7s9i06" type="submit" value="submit" form="i7s9i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s9i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s9i01" class="C1_text" disabled="" style="background-color: white;"></textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s9i1">2013/9/26 22:12:32</td>			<td class="M1_exp_item_content">	<table id="i7s9i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s9i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s9i110.png"><br>				<form id="i7s9i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s9i12" form="i7s9i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s9i14">				<input name="id" type="hidden" value="i7s9i1" form="i7s9i14">				<input name="oldgraph" type="hidden" id="i7s9i15" form="i7s9i14" value="t14i9s9i110.png">				<input id="i7s9i16" type="submit" value="submit" form="i7s9i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s9i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s9i11" class="C1_text" disabled="" style="background-color: white;">Description:
    We changed high pressure homogenizer to get cell fragments, in order to gain a more definite gel analysis. The high pressure homogenizer is under 37.5 kpSI  which supposed much too high to make protein. Even the super protein is far less compared with gel picture made after ultrasonic fragmentation, the unclear band is still there.
 Data analysis:
 In picture 1, the band marked in red was not the target band. In picture 2, the band marked in red disappeared is pSB1C3-gfp-luxI/DH5α and pSB3T5-aiiA/DH5α.
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="8. Testing our marker .(9.09)" id="sb7s10" style="background-color: rgb(166, 166, 166);">			<button class="M1_exp_step_name_button" style="background-color: rgb(166, 166, 166);"><input value="8. Testing our marker .(9.09)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:13:05--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s10i0">2013/9/26 22:13:16</td>			<td class="M1_exp_item_content">	<table id="i7s10i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s10i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s10i011.png"><br>				<form id="i7s10i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s10i02" form="i7s10i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s10i04">				<input name="id" type="hidden" value="i7s10i0" form="i7s10i04">				<input name="oldgraph" type="hidden" id="i7s10i05" form="i7s10i04" value="t14i9s10i011.png">				<input id="i7s10i06" type="submit" value="submit" form="i7s10i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s10i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s10i01" class="C1_text" disabled="" style="background-color: white;">Description:
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="8. Testing our marker .(9.09)" id="sb7s10" >			<button class="M1_exp_step_name_button" ><input value="8. Testing our marker .(9.09)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:13:05--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s10i0">2013/9/26 22:13:16</td>			<td class="M1_exp_item_content">	<table id="i7s10i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s10i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s10i011.png"><br>				<form id="i7s10i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s10i02" form="i7s10i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s10i04">				<input name="id" type="hidden" value="i7s10i0" form="i7s10i04">				<input name="oldgraph" type="hidden" id="i7s10i05" form="i7s10i04" value="t14i9s10i011.png">				<input id="i7s10i06" type="submit" value="submit" form="i7s10i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s10i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s10i01" class="C1_text" disabled="" style="background-color: white;">Description:
    We were confused for our dislocation of GFP on SDS-PAGE gel pictures for a long time. So we decided to use three different marker to confirm our marker.

Team:XMU-China/wetlab journal