The band marked in red disappeared is pSB1C3-gfp-luxI/BL21.The LuxR, GFP and AiiA are the same of size (marked in yellow). However the band of AiiA was not clear. Many target proteins were misfolded. Therefore the culture condition should be optimized.
The band marked in red disappeared is pSB1C3-gfp-luxI/BL21.The LuxR, GFP and AiiA are the same of size (marked in yellow). However the band of AiiA was not clear. Many target proteins were misfolded. Therefore the culture condition should be optimized.
We had transformed triple plasmids into one E.coli and the DNA gel picture indicated it was ligated successfully. So now we were very eager to know if our circuits could work well in E.coli.
We had transformed triple plasmids into one E.coli and the DNA gel picture indicated it was ligated successfully. So now we were very eager to know if our circuits could work well in E.coli.
Furthermore, we wanted to know that if our target proteins expressed by our constructed gene circuits, in other words, pSB1C3-gfp-luxI and pSB13T5-aiiA were aggregated in precipitation or supernatant. So we run SDS-PAGE using BL21(blank control), three cells transformed into different single plasmids(aiiA, ndh and gfp), one cell transformed into double plasmids(aiiA and gfp) and our E.coli transformed into triple plasmids in three forms respectively, whole cell, precipitation and supernatant.
Furthermore, we wanted to know that if our target proteins expressed by our constructed gene circuits, in other words, pSB1C3-gfp-luxI and pSB13T5-aiiA were aggregated in precipitation or supernatant. So we run SDS-PAGE using BL21(blank control), three cells transformed into different single plasmids(aiiA, ndh and gfp), one cell transformed into double plasmids(aiiA and gfp) and our E.coli transformed into triple plasmids in three forms respectively, whole cell, precipitation and supernatant.
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