Team:XMU-China/wetlab journal

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<div class="microfluidic">
<div class="microfluidic">
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<h1>Microfluidic Experiment Record</h1>
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<h1>Biobrick Transformation (6.24-6.30)</h1>
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<div class="M1_exp_stepInfo" title="0808 BL21(DE3) Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s0" style="background-color:#FFCE8E">
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<div class="M1_exp_stepInfo" title="Biobrick Transformation (6.24-6.30)" id="sb6s0" style=""> <button class="M1_exp_step_name_button" style=""><input value="Biobrick Transformation (6.24-6.30)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:16:17--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:16:39</td> <td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1" disabled="" style="background-color: white;">We rehydrated the registry DNA biobricks including 13-P1-12D, 13-P1-12F, 12-P2-21F, 13-P3-11H, and 12-P4-4A. Then we transformed them into BL21. After restriction analysis of the plasmids, we prepared glyoerol stocks for BL21 with 12D, 12F, and 21F.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i1">2013/9/26 20:17:26</td> <td class="M1_exp_item_content"> <table id="i6s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s0i10"  src="http://trysomething-block.stor.sinaapp.com/t14i4s0i3%E5%9B%BE%E7%89%871.jpg"><br> <form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i6s0i14"> <input name="id" type="hidden" value="i6s0i1" form="i6s0i14"> <input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i11.png"> <input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i6s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s0i11" class="C1_text" disabled="" style="background-color: white;">M:Marker DL500
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Lane1:12D plasmid
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Lane2:12D plasmid, digestion of EcoRI,SpeI
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Lane3:12D plasmid, digestion of XbaI,PstI
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Lane4:12F plasmid
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Lane5: 12F plasmid, digestion of EcoRI,SpeI
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Lane6: 12F plasmid, digestion of XbaI,PstI
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Lane7:21F plasmid
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Lane8:21F plasmid,digestion of EcoRI,SpeI
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Lane9:21F palsmid, digestion of XbaI,PstI
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table>
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<button class="M1_exp_step_name_button"><input value="0808 BL21(DE3) Gfp
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</div>
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(pSB1C3) aiia(pSB3T5)" class="M1_exp_step_name" style="background-color:#FFCE8E"></button> <span  
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<div class="microfluidic">
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<h1> Plasmids Construction (7.1-7.28, 8.15-9.16)</h1>
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<div class="M1_exp_stepInfo" title="pSB1C3-gfp-luxI (7.1-7.7)" id="sb5s0"> <button class="M1_exp_step_name_button"><input value="pSB1C3-gfp-luxI (7.1-7.7)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:36:39--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s0i0">2013/9/26 20:36:58</td> <td class="M1_exp_item_content"><textarea id="i5s0i0" class="C0" rows="1">  We purified the plasmids of 11H and 12D, after restriction analysis confirm, we inserted 11H into 12D. Next, we transformed 11H+12D into bacteria and got the plasmids to confirm. By inserting 21F into 11H+12D, we got one of our three plasmids. Finally, by using restriction analysis, we confirmed that the plasmid is right.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s0i1" style="background-color: orange;">2013/9/28 01:03:53</td> <td class="M1_exp_item_content"> <table id="i5s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s0i10"  src="http://trysomething-block.stor.sinaapp.com/t14i5s0i2图片2.jpg"><br> <form id="i5s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s0i12" form="i5s0i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s0i14"> <input name="id" type="hidden" value="i5s0i1" form="i5s0i14"> <input name="oldgraph" type="hidden" id="i5s0i15" form="i5s0i14" value="t14i5s0i2图片2.jpg"> <input id="i5s0i16" type="submit" value="submit" form="i5s0i14" class="C1_button2" disabled=""> <div id="i5s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s0i11" class="C1_text">M:Marker 1000bp
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Lane1,6,9:11H+12D 21F plasmid
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Lane2,4,7:11H+12D+21F plasmid,digestion of EcoRI
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Lane3,5,8:11H+12D+21F  plasmid,digestion of EcoRI and SpeI
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="pSB3T5-aiiA (7.11-7.28)" id="sb5s1"> <button class="M1_exp_step_name_button"><input value="pSB3T5-aiiA (7.11-7.28)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:37:51--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s1i0">2013/9/26 20:38:17</td> <td class="M1_exp_item_content"><textarea id="i5s1i0" class="C0" rows="1">  We changed 12F's backbone from pSB1C3 to pSB3T5 and pSB1T3.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s1i1">2013/9/28 01:05:10</td> <td class="M1_exp_item_content"> <table id="i5s1i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s1i10"  src="http://trysomething-block.stor.sinaapp.com/t14i5s1i3图片3.jpg"><br> <form id="i5s1i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s1i12" form="i5s1i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s1i14"> <input name="id" type="hidden" value="i5s1i1" form="i5s1i14"> <input name="oldgraph" type="hidden" id="i5s1i15" form="i5s1i14" value="t14i5s1i3图片3.jpg"> <input id="i5s1i16" type="submit" value="submit" form="i5s1i14" class="C1_button2" disabled=""> <div id="i5s1i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s1i11" class="C1_text">M:Marker 500bp
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Lane1-10:colony PCR products of  12F+3T5
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s1i2">2013/9/28 01:05:15</td> <td class="M1_exp_item_content"> <table id="i5s1i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s1i20"  src="http://trysomething-block.stor.sinaapp.com/t14i5s1i4图片4.jpg"><br> <form id="i5s1i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s1i22" form="i5s1i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s1i24"> <input name="id" type="hidden" value="i5s1i2" form="i5s1i24"> <input name="oldgraph" type="hidden" id="i5s1i25" form="i5s1i24" value="t14i5s1i4图片4.jpg"> <input id="i5s1i26" type="submit" value="submit" form="i5s1i24" class="C1_button2" disabled=""> <div id="i5s1i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s1i21" class="C1_text">M:Marker 1000bp
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Lane1:pSB3T5+aiiA plasmid
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Lane2:pSB3T5+aiiA EcoRI and SpeI digest
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Lane3:pSB3T5+aiiA XbaI and PstI digest
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="pSB4K5-ndh (7.2-7.25)" id="sb5s2"> <button class="M1_exp_step_name_button"><input value="pSB4K5-ndh (7.2-7.25)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:40:10--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s2i0">2013/9/26 20:40:27</td> <td class="M1_exp_item_content"><textarea id="i5s2i0" class="C0" rows="1">  We got ndh via PCR, and ligated it with LVA tag, but the ligation of plasmids and new backbones was failed. To solve this problem, we used new ndh whose original backbone is pSB1A2, and inserted 21F into it. The construction of ndh with pSB1K3, pSB1C3, and pSB4K5 finally finished on July 25th</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s2i1">2013/9/28 01:06:41</td> <td class="M1_exp_item_content"> <table id="i5s2i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s2i10"  src="http://trysomething-block.stor.sinaapp.com/t14i5s2i1图片5.jpg"><br> <form id="i5s2i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s2i12" form="i5s2i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s2i14"> <input name="id" type="hidden" value="i5s2i1" form="i5s2i14"> <input name="oldgraph" type="hidden" id="i5s2i15" form="i5s2i14" value="t14i5s2i1图片5.jpg"> <input id="i5s2i16" type="submit" value="submit" form="i5s2i14" class="C1_button2" disabled=""> <div id="i5s2i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s2i11" class="C1_text">M1:Marker 500bp
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Lane1-3:Fusion PCR products of ndh and lva-T
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s2i2">2013/9/28 01:06:51</td> <td class="M1_exp_item_content"> <table id="i5s2i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s2i20"  src="http://trysomething-block.stor.sinaapp.com/t14i5s2i4图片6.jpg"><br> <form id="i5s2i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s2i22" form="i5s2i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s2i24"> <input name="id" type="hidden" value="i5s2i2" form="i5s2i24"> <input name="oldgraph" type="hidden" id="i5s2i25" form="i5s2i24" value="t14i5s2i4图片6.jpg"> <input id="i5s2i26" type="submit" value="submit" form="i5s2i24" class="C1_button2" disabled=""> <div id="i5s2i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s2i21" class="C1_text">M:Marker 500bp
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Lane1:pSB4K5+ndh plasmid
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Lane2:pSB4K5+ndh EcoRI digest
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Lane3:pSB4K5+ndh EcoRI and SpeI digest
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="pSB1A3-GFP-luxI (8.15-9.5)" id="sb5s3"> <button class="M1_exp_step_name_button"><input value="pSB1A3-GFP-luxI (8.15-9.5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:43:04--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s3i0">2013/9/26 20:43:20</td> <td class="M1_exp_item_content"><textarea id="i5s3i0" class="C0" rows="1">  By protein confirm, we found that the protein our bacteria with GFP conveyed was incorrect. It was very difficult to make sure what happened to former steps. So we had to reconstruct plasmid GFP.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s3i1">2013/9/28 01:10:10</td> <td class="M1_exp_item_content"> <table id="i5s3i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s3i10"  src="http://trysomething-block.stor.sinaapp.com/t14i5s3i3图片7.jpg"><br> <form id="i5s3i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s3i12" form="i5s3i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s3i14"> <input name="id" type="hidden" value="i5s3i1" form="i5s3i14"> <input name="oldgraph" type="hidden" id="i5s3i15" form="i5s3i14" value="t14i5s3i3图片7.jpg"> <input id="i5s3i16" type="submit" value="submit" form="i5s3i14" class="C1_button2" disabled=""> <div id="i5s3i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s3i11" class="C1_text">M: Marker 1kb
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Lane1: 21F+11H+12D, digestion of EcoRI and SpeI
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Lane2: pSB1A3, digestion of EcoRI and XbaI
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s3i2">2013/9/28 01:10:38</td> <td class="M1_exp_item_content"> <table id="i5s3i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s3i20"  src="http://trysomething-block.stor.sinaapp.com/t14i5s3i4图片8.jpg"><br> <form id="i5s3i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s3i22" form="i5s3i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s3i24"> <input name="id" type="hidden" value="i5s3i2" form="i5s3i24"> <input name="oldgraph" type="hidden" id="i5s3i25" form="i5s3i24" value="t14i5s3i4图片8.jpg"> <input id="i5s3i26" type="submit" value="submit" form="i5s3i24" class="C1_button2" disabled=""> <div id="i5s3i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s3i21" class="C1_text">M: Marker 1kbp
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Lane1, 4: pSB1A3
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Lane2, 5: 21F+11H+12D
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Lane3, 6: aiiA
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="pSB1C3-sfGFP-luxI (8.23-9.7)" id="sb5s4"> <button class="M1_exp_step_name_button"><input value="pSB1C3-sfGFP-luxI (8.23-9.7)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:44:56--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s4i0">2013/9/26 20:45:14</td> <td class="M1_exp_item_content"><textarea id="i5s4i0" class="C0" rows="1">  We got sfGFP plasmid from Peking University, and to construct a complete plasmid we need, we would add a LVA tag behind sfGFP's sequence, and then construct 21F+sfGFP-LVA+12D with pSB1C3 backbone.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s4i1">2013/9/28 01:11:54</td> <td class="M1_exp_item_content"> <table id="i5s4i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s4i10"  src="http://trysomething-block.stor.sinaapp.com/t14i5s4i1图片9.jpg"><br> <form id="i5s4i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s4i12" form="i5s4i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s4i14"> <input name="id" type="hidden" value="i5s4i1" form="i5s4i14"> <input name="oldgraph" type="hidden" id="i5s4i15" form="i5s4i14" value="t14i5s4i1图片9.jpg"> <input id="i5s4i16" type="submit" value="submit" form="i5s4i14" class="C1_button2" disabled=""> <div id="i5s4i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s4i11" class="C1_text">M1: Marker 500bp
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M2:Marker DL500bp
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Lane 1,2: 12D, digestion of EcoRI and XbaI
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Lane 3,4: sfGFP+LVA, fusion PCR
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Lane 5: sfGFP, PCR
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Lane 6: LVA, PCR
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s4i2">2013/9/28 01:12:42</td> <td class="M1_exp_item_content"> <table id="i5s4i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s4i20"  src="http://trysomething-block.stor.sinaapp.com/t14i5s4i4图片10.jpg"><br> <form id="i5s4i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s4i22" form="i5s4i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s4i24"> <input name="id" type="hidden" value="i5s4i2" form="i5s4i24"> <input name="oldgraph" type="hidden" id="i5s4i25" form="i5s4i24" value="t14i5s4i4图片10.jpg"> <input id="i5s4i26" type="submit" value="submit" form="i5s4i24" class="C1_button2" disabled=""> <div id="i5s4i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s4i21" class="C1_text">M: Marker 1kbp
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Lane1, 4: 21F+sfGFP+LVA+12D, plasmid
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Lane2, 5: 21F+sfGFP+LVA+12D, digestion of EcoRI
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Lane3, 6: 21F+sfGFP+LVA+12D, digestion of EcoRI and PstI
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="p3H-GFP-luxI and p3H-sfGFP-luxI (9.7-9.16)" id="sb5s5"> <button class="M1_exp_step_name_button"><input value="p3H-GFP-luxI and p3H-sfGFP-luxI (9.7-9.16)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:47:01--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s5i0">2013/9/26 20:47:15</td> <td class="M1_exp_item_content"><textarea id="i5s5i0" class="C0" rows="1">  To make our circuit oscillate, we decided to change the backbone of GFP plasmid one more time. This time, according to reference, we needed to ligated GFP part with colE1.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s5i1">2013/9/28 01:16:48</td> <td class="M1_exp_item_content"> <table id="i5s5i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s5i10"  src="http://trysomething-block.stor.sinaapp.com/t14i5s5i4图片11.jpg"><br> <form id="i5s5i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s5i12" form="i5s5i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s5i14"> <input name="id" type="hidden" value="i5s5i1" form="i5s5i14"> <input name="oldgraph" type="hidden" id="i5s5i15" form="i5s5i14" value="t14i5s5i4图片11.jpg"> <input id="i5s5i16" type="submit" value="submit" form="i5s5i14" class="C1_button2" disabled=""> <div id="i5s5i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s5i11" class="C1_text">M:Marker 1kb
 +
Lane1:3H plasmid
 +
Lane2:3H plasmid,digestion of EcoRI
 +
Lane3:3H plasmid,digestion of EcoRI and SpeI.
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s5i2">2013/9/28 01:17:27</td> <td class="M1_exp_item_content"> <table id="i5s5i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s5i20"  src="http://trysomething-block.stor.sinaapp.com/t14i5s5i5图片12.jpg"><br> <form id="i5s5i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s5i22" form="i5s5i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s5i24"> <input name="id" type="hidden" value="i5s5i2" form="i5s5i24"> <input name="oldgraph" type="hidden" id="i5s5i25" form="i5s5i24" value="t14i5s5i5图片12.jpg"> <input id="i5s5i26" type="submit" value="submit" form="i5s5i24" class="C1_button2" disabled=""> <div id="i5s5i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s5i21" class="C1_text">M:Marker 1kb
 +
Lane1,4:3H+sfGFP plasmid
 +
Lane2,5:3H+sfGFP plasmid,digestion of XbaI
 +
Lane3,6:3H+sfGFP of XbaI and PstI
 +
Lane7,10:3H+GFP plasmid
 +
Lane8,11:3H+GFP plasmid,digestion of XbaI
 +
Lane9,12:3H+GFP plasmid,digestion of XbaI and PstI
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="aiiA/ndh+pSB3T5 (9.7-9.15)" id="sb5s6"> <button class="M1_exp_step_name_button"><input value="aiiA/ndh+pSB3T5 (9.7-9.15)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:48:37--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s6i0">2013/9/26 20:48:50</td> <td class="M1_exp_item_content"><textarea id="i5s6i0" class="C0" rows="1">  To solve the problem that plasmids could not work correctly because of the bad compatibility of backbones, we decided to insert ndh part into aiiA plasmid.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib5s6i1">2013/9/28 01:19:02</td> <td class="M1_exp_item_content"> <table id="i5s6i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i5s6i10"  src="http://trysomething-block.stor.sinaapp.com/t14i5s6i2图片13.jpg"><br> <form id="i5s6i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i5s6i12" form="i5s6i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i5s6i14"> <input name="id" type="hidden" value="i5s6i1" form="i5s6i14"> <input name="oldgraph" type="hidden" id="i5s6i15" form="i5s6i14" value="t14i5s6i2图片13.jpg"> <input id="i5s6i16" type="submit" value="submit" form="i5s6i14" class="C1_button2" disabled=""> <div id="i5s6i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i5s6i11" class="C1_text">M:Marker 1kb
 +
Lane1,6:aiiA/ndh+3T5 plasmid,digestion of XbaI and PstI
 +
Lane2,5:aiiA/ndh+3T5 plasmid,digestion of XbaI
 +
Lane3,4:aiiA/ndh+3T5 plasmid
 +
Lane7,12:aiiA+ndh plasmid,digestion of XbaI and PstI
 +
Lane8,11:aiiA+ndh plasmid,digestion of XbaI
 +
Lane9,10:aiiA+ndh plasmid
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table>
 +
</div>
-
class="M1_exp_step_time">2013/9/18 10:50:50--Chun Tang</span> </div>
 
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<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
 
-
<td class="M1_exp_item_time" id="ib1s0i0">2013/9/25 20:38:47</td>
 
-
<td class="M1_exp_item_content"> <table id="i1s0i0" class="C1"> <tbody><tr>
+
<div class="microfluidic">
 +
<h1>Transformation (7.29-9.20)</h1>
 +
<div class="M1_exp_stepInfo" title="BL21 (DE3) (7.29-8.15)" id="sb6s0"> <button class="M1_exp_step_name_button"><input value="BL21 (DE3) (7.29-8.15)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:49:56--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:50:04</td> <td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1">  Because we had constructed three plasmids we need, our next goal was to transform the plasmids into BL21(DE3). We first constructed a double-plasmid BL21: GFP+aiiA, and then we transformed the third plasmid into BL21 with two plasmids.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i1">2013/9/28 01:21:56</td> <td class="M1_exp_item_content"> <table id="i6s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s0i10" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i6s0i3图片14.jpg"><br> <form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s0i14"> <input name="id" type="hidden" value="i6s0i1" form="i6s0i14"> <input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i3图片14.jpg"> <input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled=""> <div id="i6s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s0i11" class="C1_text">M:Marker  1kb
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Lane1:GFP plasmid,digestion of EcoRI and SpeI
 +
Lane2:GFP plasmid,digestion of EcoRI
 +
Lane3:GFP plasmid
 +
Lane4:aiiA plasmid
 +
Lane5:aiiA plasmid,digestion of EcoRI
 +
Lane6:aiiA plasmid,digestion of EcoRI and SpeI
 +
Lane7:ndh plasmid,digestion of EcoRI and SpeI
 +
Lane8:ndh plasmid,digestion of EcoRI
 +
Lane9:ndh plasmid
 +
Lane10,15,16:aiiA+GFP plasmid
 +
Lane11,14,17:aiiA+GFP plasmid,digestion of EcoRI
 +
Lane12,13,18:aiiA+GFP plasmid ,digestion of EcoRI and SpeI
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s0i2">2013/9/28 01:22:37</td> <td class="M1_exp_item_content"> <table id="i6s0i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s0i20"  src="http://trysomething-block.stor.sinaapp.com/t14i6s0i4图片15.jpg"><br> <form id="i6s0i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s0i22" form="i6s0i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s0i24"> <input name="id" type="hidden" value="i6s0i2" form="i6s0i24"> <input name="oldgraph" type="hidden" id="i6s0i25" form="i6s0i24" value="t14i6s0i4图片15.jpg"> <input id="i6s0i26" type="submit" value="submit" form="i6s0i24" class="C1_button2" disabled=""> <div id="i6s0i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s0i21" class="C1_text">M：Marker 1kb
 +
Lane1: plasmids (GFP+aiiA)
 +
Lane2: three plasmids (GFP, aiiA, ndh)
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title=" DH5α (7.30-9.20)" id="sb6s1"> <button class="M1_exp_step_name_button"><input value=" DH5α (7.30-9.20)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:51:34--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s1i0">2013/9/26 20:51:47</td> <td class="M1_exp_item_content"><textarea id="i6s1i0" class="C0" rows="1">  Although we have transformed three plasmids into BL21, the result was undesirable. We found that DH5α may be more suitable for our vector, so we decided to transform the plasmids into DH5α one more time.
 +
  Later we constructed 1A3 GFP, colE1＋GFP, sfGFP, colE1＋sfGFP, and aiiA/ndh pSB3T5, so we transformed all these plasmids into DH5α for our new work of construction.
 +
</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s1i1">2013/9/28 01:23:53</td> <td class="M1_exp_item_content"> <table id="i6s1i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s1i10"  src="http://trysomething-block.stor.sinaapp.com/t14i6s1i2图片16.jpg"><br> <form id="i6s1i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s1i12" form="i6s1i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s1i14"> <input name="id" type="hidden" value="i6s1i1" form="i6s1i14"> <input name="oldgraph" type="hidden" id="i6s1i15" form="i6s1i14" value="t14i6s1i2图片16.jpg"> <input id="i6s1i16" type="submit" value="submit" form="i6s1i14" class="C1_button2" disabled=""> <div id="i6s1i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s1i11" class="C1_text">M: Marker 1kb
 +
Lane1,6,7: GFP+aiiA ndh, digestion of EcoRI and PstI
 +
Lane2,5,8: GFP+aiiA ndh, digestion of EcoRI
 +
Lane3,4,9: GFP+aiiA+ndh plasmids
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="BL21 (WT) (9.11-9.20)" id="sb6s2"> <button class="M1_exp_step_name_button"><input value="BL21 (WT) (9.11-9.20)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:52:47--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s2i0">2013/9/26 20:53:01</td> <td class="M1_exp_item_content"><textarea id="i6s2i0" class="C0" rows="1">  Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed aiiA/ndh pSB3T5, sfGFP, colE1＋GFP, colE1＋sfGFP and GFP＋pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s2i1">2013/9/28 01:24:59</td> <td class="M1_exp_item_content"> <table id="i6s2i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i6s2i10" width="450px" height="auto"  src="http://trysomething-block.stor.sinaapp.com/t14i6s2i2图片17.jpg"><br> <form id="i6s2i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i6s2i12" form="i6s2i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i6s2i14"> <input name="id" type="hidden" value="i6s2i1" form="i6s2i14"> <input name="oldgraph" type="hidden" id="i6s2i15" form="i6s2i14" value="t14i6s2i2图片17.jpg"> <input id="i6s2i16" type="submit" value="submit" form="i6s2i14" class="C1_button2" disabled=""> <div id="i6s2i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i6s2i11" class="C1_text">M:Marker 1kb
 +
Lane1,4 :GFP(1A3)＋aiiA+ndh plasmid
 +
Lane2,5 : GFP(1A3)＋aiiA+ndh plasmid,digestion of XbaI
 +
Lane3,6 : GFP(1A3)＋aiiA+ndh plasmid,digestion of XbaI and PstI
 +
Lane7: GFP(3H) aiiA+ndh plasmid
 +
Lane8: GFP(3H) aiiA+ndh plasmid digestion of XbaI
 +
Lane9: GFP(3H) aiiA+ndh plasmid digestion of XbaI and PstI
 +
Lane10: sfGFP(1C3)＋aiiA+ndh plasmid
 +
Lane11: sfGFP(1C3)＋aiiA+ndh plasmid digestion of XbaI
 +
Lane12: sfGFP(1C3)＋aiiA+ndh plasmid digestion of XbaI and PstI
 +
Lane13: sfGFP(3H)＋aiiA+ndh plasmid
 +
Lane14: sfGFP(3H)＋aiiA+ndh plasmid digestion of XbaI
 +
Lane15: sfGFP(3H)＋aiiA+ndh plasmid digestion of XbaI and PstI
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="MG1655 (9.11-9.21)" id="sb6s3"> <button class="M1_exp_step_name_button"><input value="MG1655 (9.11-9.21)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 20:55:54--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib6s3i0">2013/9/26 20:56:19</td> <td class="M1_exp_item_content"><textarea id="i6s3i0" class="C0" rows="1">  Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed sfGFP, colE1＋GFP, and colE1＋sfGFP, GFP＋pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td> </tr> </tbody></table> </div>
-
<td class="C1_td"> <img id="i1s0i00"  
+
<div class="microfluidic">
 +
<h1>SDS-PAGE</h1>
 +
<div class="M1_exp_stepInfo" title="Abbreviation:" id="sb7s0" > <button class="M1_exp_step_name_button" ><input value="Abbreviation:" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 21:48:57--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s0i0">2013/9/26 21:49:44</td> <td class="M1_exp_item_content"><textarea id="i7s0i0" class="C0" rows="1" disabled="" style="background-color: white;">W: Whole cell  P: Precipitation  S: Supernatant
 +
</textarea></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="Background" id="sb7s1" > <button class="M1_exp_step_name_button" ><input value="Background" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 21:50:08--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s1i0">2013/9/26 21:51:07</td> <td class="M1_exp_item_content"><table id="i7s1i0" class="C3"><tbody><tr><td class="C3_td"><input value="CDS" class="C3_input"></td><td class="C3_td"><input value="Size(bp)" class="C3_input"></td><td class="C3_td"><input value="Protein" class="C3_input"></td><td class="C3_td"><input value="Size(kDa)" class="C3_input"></td></tr><tr><td class="C3_td"><input value="luxR" class="C3_input"></td><td class="C3_td"><input value="750" class="C3_input"></td><td class="C3_td"><input value="LuxR" class="C3_input"></td><td class="C3_td"><input value="27.5" class="C3_input"></td></tr><tr><td class="C3_td"><input value="gfp-lva" class="C3_input"></td><td class="C3_td"><input value="753" class="C3_input"></td><td class="C3_td"><input value="GFP-LVA" class="C3_input"></td><td class="C3_td"><input value="27.61" class="C3_input"></td></tr><tr><td class="C3_td"><input value="aiiA-lva" class="C3_input"></td><td class="C3_td"><input value="783" class="C3_input"></td><td class="C3_td"><input value="aiiA-LVA" class="C3_input"></td><td class="C3_td"><input value="28.71" class="C3_input"></td></tr><tr><td class="C3_td"><input value="luxI-lva" class="C3_input"></td><td class="C3_td"><input value="612" class="C3_input"></td><td class="C3_td"><input value="LuxI-LVA" class="C3_input"></td><td class="C3_td"><input value="22.44" class="C3_input"></td></tr><tr><td class="C3_td"><input value="ndh-lva" class="C3_input"></td><td class="C3_td"><input value="1335" class="C3_input"></td><td class="C3_td"><input value="NDH-LVA" class="C3_input"></td><td class="C3_td"><input value="48.95" class="C3_input"></td></tr></tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="Graphic Symbol" id="sb7s2" > <button class="M1_exp_step_name_button" ><input value="Graphic Symbol" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 21:53:17--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s2i0">2013/9/26 21:55:45</td> <td class="M1_exp_item_content"><table id="i7s2i0" class="C3"><tbody><tr><td class="C3_td"><input value="Strain" class="C3_input"></td><td class="C3_td"><input value="Plasmids" class="C3_input"></td><td class="C3_td"><input value="Proteins" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td><td class="C3_td"><input value="NDH" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp-luxI" class="C3_input"></td><td class="C3_td"><input value="Luxr,LuxI-LVA,GFP-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-B" class="C3_input"></td><td class="C3_td"><input value="pSB3T5-aiiA" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-C" class="C3_input"></td><td class="C3_td"><input value="pSB4K5-ndh" class="C3_input"></td><td class="C3_td"><input value="/" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A B" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp-luxI pSB3T5-aiiA" class="C3_input"></td><td class="C3_td"><input value="Luxr,LuxI-LVA,GFP,aiiA-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="BL21-A B C" class="C3_input"></td><td class="C3_td"><input value="pSB1C3-gfp luxI pSB3T5-aiiA pSB4K5-ndh" class="C3_input"></td><td class="C3_td"><input value="LuxR,LuxI-LVA,GFP-LVA.aiiA-LVA.NDH-LVA" class="C3_input"></td></tr><tr><td class="C3_td"><input value="" class="C3_input"></td><td class="C3_td"><input value="" class="C3_input"></td><td class="C3_td"><input value="" class="C3_input"></td></tr></tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="1. Characterization of expression of three single plasmids in BL21. (8.02-8. 08)" id="sb7s3" > <button class="M1_exp_step_name_button" ><input value="1. Characterization of expression of three single plasmids in BL21. (8.02-8. 08)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:02:08--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s3i0">2013/9/26 22:03:34</td> <td class="M1_exp_item_content"> <table id="i7s3i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s3i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s3i01.png"><br> <form id="i7s3i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s3i02" form="i7s3i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s3i04"> <input name="id" type="hidden" value="i7s3i0" form="i7s3i04"> <input name="oldgraph" type="hidden" id="i7s3i05" form="i7s3i04" value="t14i9s3i01.png"> <input id="i7s3i06" type="submit" value="submit" form="i7s3i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s3i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s3i01" class="C1_text" disabled="" style="background-color: white;">  We compared protein bands between BL21, the blank control and three groups transformed into different single plasmids, aiiA, ndh and gfp. These two gel pictures were got in two different days.
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Data analysis:
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  The target proteins of pSB3T5-aiiA/BL21 and pSB4K5-ndh/BL21 should not be expressed because the promoter should be induced by AHL. The target proteins of pSB1C3-gfp-luxI/BL21 have been expressed but GFP was misfolded we suppose. However, the band marked in red was not the target band. Maybe it's the complex of AHL-LuxI.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="2. Characterization of expression of double plasmids (8.05)" id="sb7s4" > <button class="M1_exp_step_name_button" ><input value="2. Characterization of expression of double plasmids (8.05)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:04:18--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s4i0">2013/9/26 22:07:07</td> <td class="M1_exp_item_content"> <table id="i7s4i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s4i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s4i02.png"><br> <form id="i7s4i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s4i02" form="i7s4i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s4i04"> <input name="id" type="hidden" value="i7s4i0" form="i7s4i04"> <input name="oldgraph" type="hidden" id="i7s4i05" form="i7s4i04" value="t14i9s4i02.png"> <input id="i7s4i06" type="submit" value="submit" form="i7s4i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s4i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s4i01" class="C1_text" disabled="" style="background-color: white;">  We performed a SDS-PAGE comparing expression of double plasmids, gfp and aiiA, and two corresponding single plasmids. What's more, we wanted to find more details about band which possibly is the misfolded gfp.
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Data analysis:
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  The band marked in red disappeared is pSB1C3-gfp-luxI/BL21.The LuxR, GFP and AiiA  are the same of size (marked in yellow). However the band of AiiA was not clear. Many target proteins were misfolded. Therefore the culture condition should be optimized.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="3. (8.12)" id="sb7s5" > <button class="M1_exp_step_name_button" ><input value="3. (8.12)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:07:33--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s5i0">2013/9/26 22:08:04</td> <td class="M1_exp_item_content"><textarea id="i7s5i0" class="C0" rows="1" disabled="" style="background-color: white;"> Characterization of expression of E.coli transformed into triple plasmids and testing if our target proteins expressed by our constructed gene circuits were concentrated in precipitation or supernatant  (8.12)</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s5i1">2013/9/26 22:07:46</td> <td class="M1_exp_item_content"> <table id="i7s5i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s5i10" width="600" src="http://trysomething-block.stor.sinaapp.com/t14i9s5i03.png"><br> <form id="i7s5i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s5i12" form="i7s5i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s5i14"> <input name="id" type="hidden" value="i7s5i1" form="i7s5i14"> <input name="oldgraph" type="hidden" id="i7s5i15" form="i7s5i14" value="t14i9s5i03.png"> <input id="i7s5i16" type="submit" value="submit" form="i7s5i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s5i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s5i11" class="C1_text" disabled="" style="background-color: white;">Description :
 +
We had transformed triple plasmids into one E.coli and the DNA gel picture indicated it was ligated successfully. So now we were very eager to know if our circuits could work well in E.coli.
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Furthermore, we wanted to know that if our target proteins expressed by our constructed gene circuits, in other words, pSB1C3-gfp-luxI and pSB13T5-aiiA were aggregated in precipitation or supernatant. So we run SDS-PAGE using BL21(blank control), three cells transformed into different single plasmids(aiiA, ndh and gfp), one cell transformed into double plasmids(aiiA and gfp) and our E.coli transformed into triple plasmids in three forms respectively, whole cell, precipitation and supernatant.
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class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s0i5808.gif"><br>
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Data analysis:
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  From the SDS-PAGE gel pictures, we could easily find that the clear AiiA band (marked in red) but NDH was not clear in PAGE because the gene ndh did exist in the genome DNA of BL21 native strain.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="4. Characterization of expression of single plasmid in DH5α(8.17)" id="sb7s6" > <button class="M1_exp_step_name_button" ><input value="4. Characterization of expression of single plasmid in DH5α(8.17)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:08:31--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s6i0">2013/9/26 22:08:47</td> <td class="M1_exp_item_content"> <table id="i7s6i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s6i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s6i04.png"><br> <form id="i7s6i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s6i02" form="i7s6i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s6i04"> <input name="id" type="hidden" value="i7s6i0" form="i7s6i04"> <input name="oldgraph" type="hidden" id="i7s6i05" form="i7s6i04" value="t14i9s6i04.png"> <input id="i7s6i06" type="submit" value="submit" form="i7s6i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s6i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s6i01" class="C1_text" disabled="" style="background-color: white;">  We've found that maybe there's some mistakes in our strains which cause us couldn't get a expected microfluidic experiment result. And we analyzed that DH5α maybe a better strain than BL21 so we changed the host. We firstly confirm the expression of three single plasmids transformed into DH5α.
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<form id="i1s0i04" target="M7" action="img.php" method="post"  
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Data analysis:
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The band marked in red was not the target band. Maybe it is the complex of AHL-LuxI. The target proteins of pSB1C3-gfp-luxI have been expressed while the target proteins of pSB3T5-aiiA and pSB4K5-ndh did not be  expressed because the promoter should be induced by AHL.
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" > <button class="M1_exp_step_name_button" ><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td> <td class="M1_exp_item_content"> <table id="i7s7i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br> <form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i04"> <input name="id" type="hidden" value="i7s7i0" form="i7s7i04"> <input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png"> <input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;">  We were suggested that DH5α has a better viability and a higher protein expression. So  there's some mistakes in our strains which cause us couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
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Data analysis:
 +
E.coli maybe cultured at 30℃ had a stronger expression of protein from picture 1 but we were confused by picture 2 cause it indicated that expression at 30℃ is not larger than expression at 37℃.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s7i1">2013/9/26 22:09:40</td> <td class="M1_exp_item_content"> <table id="i7s7i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s7i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i16.png"><br> <form id="i7s7i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s7i12" form="i7s7i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s7i14"> <input name="id" type="hidden" value="i7s7i1" form="i7s7i14"> <input name="oldgraph" type="hidden" id="i7s7i15" form="i7s7i14" value="t14i9s7i16.png"> <input id="i7s7i16" type="submit" value="submit" form="i7s7i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s7i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s7i11" class="C1_text" disabled="" style="background-color: white;">Future plan:
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1.It's much better to culture at 30℃ rather than 37℃.
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2.Does it better for us to use superfolded GFP?
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3.Change another ultrasonic instrument or high pressure homogenizer.
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enctype="multipart/form-data"></form> <input name="img"  
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</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="6. Repeat SDS-PAGE with optimized conditions.(9.02)" id="sb7s8" > <button class="M1_exp_step_name_button" ><input value="6. Repeat SDS-PAGE with optimized conditions.(9.02)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:10:13--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s8i0">2013/9/26 22:10:54</td> <td class="M1_exp_item_content"> <table id="i7s8i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s8i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s8i07.png"><br> <form id="i7s8i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s8i02" form="i7s8i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s8i04"> <input name="id" type="hidden" value="i7s8i0" form="i7s8i04"> <input name="oldgraph" type="hidden" id="i7s8i05" form="i7s8i04" value="t14i9s8i07.png"> <input id="i7s8i06" type="submit" value="submit" form="i7s8i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s8i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s8i01" class="C1_text" disabled="" style="background-color: white;"></textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s8i1">2013/9/26 22:11:28</td> <td class="M1_exp_item_content"> <table id="i7s8i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s8i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s8i18.png"><br> <form id="i7s8i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s8i12" form="i7s8i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s8i14"> <input name="id" type="hidden" value="i7s8i1" form="i7s8i14"> <input name="oldgraph" type="hidden" id="i7s8i15" form="i7s8i14" value="t14i9s8i18.png"> <input id="i7s8i16" type="submit" value="submit" form="i7s8i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s8i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s8i11" class="C1_text" disabled="" style="background-color: white;">Description:
 +
  We changed high pressure homogenizer to get cell fragments, in order to gain a more definite gel analysis. The high pressure homogenizer is under 37.5 kpSI  which supposed much too high to make protein. Even the super protein is far less compared with gel picture made after ultrasonic fragmentation, the unclear band is still there.
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type="file" id="i1s0i02" form="i1s0i04" class="C1_button1" required="required" disabled="">
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Data analysis:
 +
The band marked in red was not the target band. The band marked in green was not the target band.
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="7. Repeat SDS-PAGE with optimized conditions.(9.03)" id="sb7s9" > <button class="M1_exp_step_name_button" ><input value="7. Repeat SDS-PAGE with optimized conditions.(9.03)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:12:08--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s9i0">2013/9/26 22:12:23</td> <td class="M1_exp_item_content"> <table id="i7s9i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s9i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s9i09.png"><br> <form id="i7s9i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s9i02" form="i7s9i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s9i04"> <input name="id" type="hidden" value="i7s9i0" form="i7s9i04"> <input name="oldgraph" type="hidden" id="i7s9i05" form="i7s9i04" value="t14i9s9i09.png"> <input id="i7s9i06" type="submit" value="submit" form="i7s9i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s9i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s9i01" class="C1_text" disabled="" style="background-color: white;"></textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s9i1">2013/9/26 22:12:32</td> <td class="M1_exp_item_content"> <table id="i7s9i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s9i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s9i110.png"><br> <form id="i7s9i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s9i12" form="i7s9i14" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s9i14"> <input name="id" type="hidden" value="i7s9i1" form="i7s9i14"> <input name="oldgraph" type="hidden" id="i7s9i15" form="i7s9i14" value="t14i9s9i110.png"> <input id="i7s9i16" type="submit" value="submit" form="i7s9i14" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s9i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s9i11" class="C1_text" disabled="" style="background-color: white;">Description:
 +
  We changed high pressure homogenizer to get cell fragments, in order to gain a more definite gel analysis. The high pressure homogenizer is under 37.5 kpSI  which supposed much too high to make protein. Even the super protein is far less compared with gel picture made after ultrasonic fragmentation, the unclear band is still there.
-
<input name="pname" type="hidden" value="t14" form="i1s0i04">
+
Data analysis:
 +
In picture 1, the band marked in red was not the target band. In picture 2, the band marked in red disappeared is pSB1C3-gfp-luxI/DH5α and pSB3T5-aiiA/DH5α.
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="8. Testing our marker .(9.09)" id="sb7s10" > <button class="M1_exp_step_name_button" ><input value="8. Testing our marker .(9.09)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/26 22:13:05--Tang Chun</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s10i0">2013/9/26 22:13:16</td> <td class="M1_exp_item_content"> <table id="i7s10i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s10i00" src="http://trysomething-block.stor.sinaapp.com/t14i9s10i011.png"><br> <form id="i7s10i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s10i02" form="i7s10i04" class="C1_button1" required="required" disabled="" style="background-color: white;"> <input name="pname" type="hidden" value="t14" form="i7s10i04"> <input name="id" type="hidden" value="i7s10i0" form="i7s10i04"> <input name="oldgraph" type="hidden" id="i7s10i05" form="i7s10i04" value="t14i9s10i011.png"> <input id="i7s10i06" type="submit" value="submit" form="i7s10i04" class="C1_button2" disabled="" style="background-color: white;"> <div id="i7s10i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s10i01" class="C1_text" disabled="" style="background-color: white;">Description:
 +
  We were confused for our dislocation of GFP on SDS-PAGE gel pictures for a long time. So we decided to use three different marker to confirm our marker.
-
<input name="id" type="hidden" value="i1s0i0" form="i1s0i04">
+
Data analysis:
 +
There is a paradox between A A and A:
 +
E.coli cultured has a stronger expression of protein when not added AHL？
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title=" 9. . Characterization of ndh using additional AHL .(9.19)" id="sb7s11"> <button class="M1_exp_step_name_button"><input value=" 9. . Characterization of ndh using additional AHL .(9.19)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/28 07:03:20--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s11i0">2013/9/28 07:03:54</td> <td class="M1_exp_item_content"> <table id="i7s11i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s11i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s11i00919.png"><br> <form id="i7s11i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s11i02" form="i7s11i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s11i04"> <input name="id" type="hidden" value="i7s11i0" form="i7s11i04"> <input name="oldgraph" type="hidden" id="i7s11i05" form="i7s11i04" value="t14i7s11i00919.png"> <input id="i7s11i06" type="submit" value="submit" form="i7s11i04" class="C1_button2" disabled=""> <div id="i7s11i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s11i01" class="C1_text">Description:
 +
  We characterize our biobricks using additional AHL. We bought commercial AHL powder and prepared it into solution. The AHL added into these cells was 1000 nmol/L and BL21 was wild type.
-
<input name="oldgraph" type="hidden" id="i1s0i05" form="i1s0i04"  
+
Data analysis:
 +
The band marked in yellow disappeared.
 +
There is a difference between N and N+A：
 +
The band marked in green/red shows that  E.coli cultured has a stronger expression of protein when  added AHL?
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="10.Characterization of several parts using additional AHL .(9.27)" id="sb7s12"> <button class="M1_exp_step_name_button"><input value="10.Characterization of several parts using additional AHL .(9.27)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/28 08:44:02--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i0">2013/9/28 08:44:22</td> <td class="M1_exp_item_content"> <table id="i7s12i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i0sds1.png"><br> <form id="i7s12i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i02" form="i7s12i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i04"> <input name="id" type="hidden" value="i7s12i0" form="i7s12i04"> <input name="oldgraph" type="hidden" id="i7s12i05" form="i7s12i04" value="t14i7s12i0sds1.png"> <input id="i7s12i06" type="submit" value="submit" form="i7s12i04" class="C1_button2" disabled=""> <div id="i7s12i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i01" class="C1_text">  We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp-aiiA, sfgfp-aiiA-ndh. SDS-PAGE gel pictures are shown as follows.
-
value="t14i1s0i5808.gif"> <input id="i1s0i06" type="submit"  
+
Illustration:
 +
A:BL21(WT)
 +
B:BL21(WT) 1C3-sfgfp
 +
C:BL21(WT) 1C3-sfgfp-aiiA
 +
D:BL21(WT) 1C3-sfgfp-aiiA-ndh
 +
E:3H-1C3-sfgfp-aiiA-ndh</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i1">2013/9/28 08:47:11</td> <td class="M1_exp_item_content"> <table id="i7s12i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i10" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i4sds2.png"><br> <form id="i7s12i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i12" form="i7s12i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i14"> <input name="id" type="hidden" value="i7s12i1" form="i7s12i14"> <input name="oldgraph" type="hidden" id="i7s12i15" form="i7s12i14" value="t14i7s12i4sds2.png"> <input id="i7s12i16" type="submit" value="submit" form="i7s12i14" class="C1_button2" disabled=""> <div id="i7s12i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i11" class="C1_text">  We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp  aiiA, sfgfp  aiiA ndh. SDS-PAGE gel pictures are shown as follows.
-
value="submit" form="i1s0i04" class="C1_button2" disabled=""> <div  
+
Illustration:
 +
A:BL21(WT)
 +
B:BL21(WT) 1C3-sfgfp
 +
C:BL21(WT) 1C3-sfgfp-aiiA
 +
D:BL21(WT) 1C3-sfgfp-aiiA-ndh
 +
E:3H-1C3-sfgfp-aiiA-ndh</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i2">2013/9/28 08:44:24</td> <td class="M1_exp_item_content"> <table id="i7s12i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i20" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i1sds3.png"><br> <form id="i7s12i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i22" form="i7s12i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i24"> <input name="id" type="hidden" value="i7s12i2" form="i7s12i24"> <input name="oldgraph" type="hidden" id="i7s12i25" form="i7s12i24" value="t14i7s12i1sds3.png"> <input id="i7s12i26" type="submit" value="submit" form="i7s12i24" class="C1_button2" disabled=""> <div id="i7s12i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i21" class="C1_text">  We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp  aiiA, sfgfp  aiiA ndh. SDS-PAGE gel pictures are shown as follows.
-
id="i1s0i03" class="M1_exp_img"></div> </td> <td  
+
Illustration:
 +
A:BL21(WT)
 +
B:BL21(WT) 1C3-sfgfp
 +
C:BL21(WT) 1C3-sfgfp-aiiA
 +
D:BL21(WT) 1C3-sfgfp-aiiA-ndh
 +
E:3H-1C3-sfgfp-aiiA-ndh</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i3">2013/9/28 08:44:24</td> <td class="M1_exp_item_content"> <table id="i7s12i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i30" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i2sds4.png"><br> <form id="i7s12i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i32" form="i7s12i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i34"> <input name="id" type="hidden" value="i7s12i3" form="i7s12i34"> <input name="oldgraph" type="hidden" id="i7s12i35" form="i7s12i34" value="t14i7s12i2sds4.png"> <input id="i7s12i36" type="submit" value="submit" form="i7s12i34" class="C1_button2" disabled=""> <div id="i7s12i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i31" class="C1_text">  We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp  aiiA, sfgfp  aiiA ndh. SDS-PAGE gel pictures are shown as follows.
-
class="C1_td_text"> <textarea id="i1s0i01" class="C1_text" >8th
+
Illustration:
 +
A:BL21(WT)
 +
B:BL21(WT) 1C3-sfgfp
 +
C:BL21(WT) 1C3-sfgfp-aiiA
 +
D:BL21(WT) 1C3-sfgfp-aiiA-ndh
 +
E:3H-1C3-sfgfp-aiiA-ndh</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib7s12i4">2013/9/28 08:44:28</td> <td class="M1_exp_item_content"> <table id="i7s12i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i7s12i40" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i3sds5.png"><br> <form id="i7s12i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i7s12i42" form="i7s12i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i7s12i44"> <input name="id" type="hidden" value="i7s12i4" form="i7s12i44"> <input name="oldgraph" type="hidden" id="i7s12i45" form="i7s12i44" value="t14i7s12i3sds5.png"> <input id="i7s12i46" type="submit" value="submit" form="i7s12i44" class="C1_button2" disabled=""> <div id="i7s12i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i7s12i41" class="C1_text">Description:
 +
  We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp  aiiA, sfgfp  aiiA ndh. SDS-PAGE gel pictures are shown as follows.
-
August
+
Illustration:
-
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
+
A:BL21(WT)
 +
B:BL21(WT) 1C3-sfgfp
 +
C:BL21(WT) 1C3-sfgfp-aiiA
 +
D:BL21(WT) 1C3-sfgfp-aiiA-ndh
 +
E:3H-1C3-sfgfp-aiiA-ndh
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table>
 +
</div>
 +
 
 +
 
 +
<div class="microfluidic">
 +
<h1>Microfluidic Experiment Record</h1>
 +
<div class="M1_exp_stepInfo" title="0808 BL21(DE3) gfp(pSB1C3)+aiiA(pSB3T5)" id="sb0s0"> <button class="M1_exp_step_name_button"><input value="0808 BL21(DE3) gfp(pSB1C3)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/18 10:50:50--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s0i0">2013/9/25 20:38:47</td> <td class="M1_exp_item_content"> <table id="i0s0i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s0i00"  src="http://trysomething-block.stor.sinaapp.com/t14i1s0i5808.gif"><br> <form id="i0s0i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s0i02" form="i0s0i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s0i04"> <input name="id" type="hidden" value="i0s0i0" form="i0s0i04"> <input name="oldgraph" type="hidden" id="i0s0i05" form="i0s0i04" value="t14i1s0i5808.gif"> <input id="i0s0i06" type="submit" value="submit" form="i0s0i04" class="C1_button2" disabled=""> <div id="i0s0i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s0i01" class="C1_text">8th August
 +
Plasmid:gfp(pSB1C3)+aiiA(pSB3T5)
Bacteria:BL21(DE3)
Bacteria:BL21(DE3)
-
Condition: No flowing medium culture supply. Cells trap in the micro pools. Without  
+
Condition: No flowing medium culture supply. Cells trap in the micro pools. Without antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
antibiotic putting in.
+
Total Obserbation Time:200mins
Total Obserbation Time:200mins
Analysis:  
Analysis:  
-
At the beginning, the population of cells is small and the fluorescence degree is as the  
+
At the beginning, the population of cells was small and the fluorescence degree was as the same. Minutes later, more cells grew as well as stronger fluorescence was detected. From then on, we observed weak oscillation of the fluorescence intensity.
-
 
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="0809 BL21(DE3)  gfp(pSB1C3)+aiiA(pSB3T5)" id="sb0s1"> <button class="M1_exp_step_name_button"><input value="0809 BL21(DE3)  gfp(pSB1C3)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/18 10:51:01--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s1i0">2013/9/25 20:42:38</td> <td class="M1_exp_item_content"> <table id="i0s1i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s1i00" src="http://trysomething-block.stor.sinaapp.com/t14i1s1i3809.gif"><br> <form id="i0s1i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s1i02" form="i0s1i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s1i04"> <input name="id" type="hidden" value="i0s1i0" form="i0s1i04"> <input name="oldgraph" type="hidden" id="i0s1i05" form="i0s1i04" value="t14i1s1i3809.gif"> <input id="i0s1i06" type="submit" value="submit" form="i0s1i04" class="C1_button2" disabled=""> <div id="i0s1i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s1i01" class="C1_text">choose the photo yo9th August
-
same. Minutes later, more cells grew as well as stronger fluorescence was detected. From  
+
Plasmid:gfp(pSB1C3)+aiiA(pSB3T5)
-
 
+
-
then on, we observed weak oscillation of the fluorescence intensity.
+
-
</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="0809 BL21(DE3)  Gfp(pSB1C3) aiia(pSB3T5)"  
+
-
 
+
-
id="sb1s1"> <button class="M1_exp_step_name_button"><input value="0809  
+
-
 
+
-
BL21(DE3)  Gfp(pSB1C3) aiia(pSB3T5)" class="M1_exp_step_name"></button>
+
-
 
+
-
<span class="M1_exp_step_time">2013/9/18 10:51:01--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s1i0">2013/9/25 20:42:38</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s1i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s1i00"
+
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+
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class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s1i3809.gif"><br>
+
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+
-
<form id="i1s1i04" target="M7" action="img.php" method="post"  
+
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+
-
enctype="multipart/form-data"></form> <input name="img"  
+
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+
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type="file" id="i1s1i02" form="i1s1i04" class="C1_button1" required="required" disabled="">
+
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+
-
<input name="pname" type="hidden" value="t14" form="i1s1i04">
+
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+
-
<input name="id" type="hidden" value="i1s1i0" form="i1s1i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s1i05" form="i1s1i04"  
+
-
 
+
-
value="t14i1s1i3809.gif"> <input id="i1s1i06" type="submit"  
+
-
 
+
-
value="submit" form="i1s1i04" class="C1_button2" disabled=""> <div  
+
-
 
+
-
id="i1s1i03" class="M1_exp_img"></div> </td> <td  
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s1i01"  
+
-
 
+
-
class="C1_text">9th August
+
-
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
+
Bacteria:BL21(DE3)
Bacteria:BL21(DE3)
-
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic  
+
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
putting in.
+
Total Obserbation Time:140mins
Total Obserbation Time:140mins
Analysis:  
Analysis:  
-
At the beginning, the population of cells is small and the fluorescence degree is as the  
+
At the beginning, the population of cells was small and the fluorescence degree was as the same. Minutes later, more cells grew but the population was still not large enough to observe convictive fluorescence intensity changing.
-
 
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="0825 DH5a gfp(pSB1C3)+aiiA(pSB3T5)" id="sb0s2"> <button class="M1_exp_step_name_button"><input value="0825 DH5a gfp(pSB1C3)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/18 10:51:41--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s2i0">2013/9/25 20:45:15</td> <td class="M1_exp_item_content"> <table id="i0s2i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s2i00" src="http://trysomething-block.stor.sinaapp.com/t14i1s2i0825.gif"><br> <form id="i0s2i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s2i02" form="i0s2i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s2i04"> <input name="id" type="hidden" value="i0s2i0" form="i0s2i04"> <input name="oldgraph" type="hidden" id="i0s2i05" form="i0s2i04" value="t14i1s2i0825.gif"> <input id="i0s2i06" type="submit" value="submit" form="i0s2i04" class="C1_button2" disabled=""> <div id="i0s2i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s2i01" class="C1_text">25th August
-
same. Minutes later, more cells grew but the population is still not large enough to observe  
+
Plasmid:gfp(pSB1C3)+aiiA(pSB3T5)
-
 
+
-
convictive fluorescence intensity changing.
+
-
</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="0825 DH5a Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s2">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="0825 DH5a Gfp(pSB1C3)  
+
-
 
+
-
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span  
+
-
 
+
-
class="M1_exp_step_time">2013/9/18 10:51:41--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s2i0">2013/9/25 20:45:15</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s2i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s2i00"
+
-
 
+
-
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s2i0825.gif"><br>
+
-
 
+
-
<form id="i1s2i04" target="M7" action="img.php" method="post"  
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"  
+
-
 
+
-
type="file" id="i1s2i02" form="i1s2i04" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t14" form="i1s2i04">
+
-
 
+
-
<input name="id" type="hidden" value="i1s2i0" form="i1s2i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s2i05" form="i1s2i04"  
+
-
 
+
-
value="t14i1s2i0825.gif"> <input id="i1s2i06" type="submit"  
+
-
 
+
-
value="submit" form="i1s2i04" class="C1_button2" disabled=""> <div  
+
-
 
+
-
id="i1s2i03" class="M1_exp_img"></div> </td> <td  
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s2i01" class="C1_text">25th  
+
-
 
+
-
August
+
-
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
+
Bacteria:DH5a
Bacteria:DH5a
-
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic  
+
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
putting in.
+
Total Obserbation Time:165mins
Total Obserbation Time:165mins
Analysis:  
Analysis:  
-
At the beginning, the population of cells is small and the fluorescence degree is as the  
+
At the beginning, the population of cells was small and the fluorescence degree was as the same. Minutes later, more cells grew and we detected stronger fluorescence degree. From then on, the fluorescence degree kept in almost the same intensity.
-
 
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="0831 DH5a gfp(pSB1C3)+aiiA(pSB3T5)" id="sb0s3"> <button class="M1_exp_step_name_button"><input value="0831 DH5a gfp(pSB1C3)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/18 10:52:00--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s3i0">2013/9/25 20:46:04</td> <td class="M1_exp_item_content"> <table id="i0s3i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s3i00" src="http://trysomething-block.stor.sinaapp.com/t14i1s3i0831.gif"><br> <form id="i0s3i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s3i02" form="i0s3i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s3i04"> <input name="id" type="hidden" value="i0s3i0" form="i0s3i04"> <input name="oldgraph" type="hidden" id="i0s3i05" form="i0s3i04" value="t14i1s3i0831.gif"> <input id="i0s3i06" type="submit" value="submit" form="i0s3i04" class="C1_button2" disabled=""> <div id="i0s3i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s3i01" class="C1_text">31th Auguest
-
same. Minutes later, more cells grew and we detect stronger fluorescence degree. From then  
+
Plasmid:gfp(pSB1C3)+aiiA(pSB3T5)
-
 
+
-
on, the fluorescence degree kept in almost the same intensity.
+
-
</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="0831 DH5a Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s3">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="0831 DH5a Gfp(pSB1C3)  
+
-
 
+
-
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span  
+
-
 
+
-
class="M1_exp_step_time">2013/9/18 10:52:00--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s3i0">2013/9/25 20:46:04</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s3i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s3i00"
+
-
 
+
-
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s3i0831.gif"><br>
+
-
 
+
-
<form id="i1s3i04" target="M7" action="img.php" method="post"  
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"  
+
-
 
+
-
type="file" id="i1s3i02" form="i1s3i04" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t14" form="i1s3i04">
+
-
 
+
-
<input name="id" type="hidden" value="i1s3i0" form="i1s3i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s3i05" form="i1s3i04"  
+
-
 
+
-
value="t14i1s3i0831.gif"> <input id="i1s3i06" type="submit"  
+
-
 
+
-
value="submit" form="i1s3i04" class="C1_button2" disabled=""> <div  
+
-
 
+
-
id="i1s3i03" class="M1_exp_img"></div> </td> <td  
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s3i01" class="C1_text">31th  
+
-
 
+
-
Auguest
+
-
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
+
Bacteria:DH5a
Bacteria:DH5a
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
antibiotic putting in.
+
Total Obserbation Time:80mins
Total Obserbation Time:80mins
Analysis:  
Analysis:  
-
At the beginning, the population of cell was large enough to get valid fluorescence degree.  
+
At the beginning, the population of cell was large enough to get valid fluorescence degree. And from then on , we observed no fluorescence intensity changing.
-
 
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="0912 DH5a gfp(pSB1C3)+aiiA(pSB3T5)" id="sb0s4"> <button class="M1_exp_step_name_button"><input value="0912 DH5a gfp(pSB1C3)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/18 10:52:31--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s4i0">2013/9/25 20:47:42</td> <td class="M1_exp_item_content"> <table id="i0s4i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s4i00" src="http://trysomething-block.stor.sinaapp.com/t14i1s4i0912.gif"><br> <form id="i0s4i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s4i02" form="i0s4i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s4i04"> <input name="id" type="hidden" value="i0s4i0" form="i0s4i04"> <input name="oldgraph" type="hidden" id="i0s4i05" form="i0s4i04" value="t14i1s4i0912.gif"> <input id="i0s4i06" type="submit" value="submit" form="i0s4i04" class="C1_button2" disabled=""> <div id="i0s4i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s4i01" class="C1_text">12th September
-
And from then on , we observed no fluorescence intensity changing.
+
Plasmid:gfp(pSB1C3)+aiiA(pSB3T5)
-
</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="0912 DH5a Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s4">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="0912 DH5a Gfp(pSB1C3)  
+
-
 
+
-
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span  
+
-
 
+
-
class="M1_exp_step_time">2013/9/18 10:52:31--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s4i0">2013/9/25 20:47:42</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s4i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s4i00"
+
-
 
+
-
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s4i0912.gif"><br>
+
-
 
+
-
<form id="i1s4i04" target="M7" action="img.php" method="post"  
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"  
+
-
 
+
-
type="file" id="i1s4i02" form="i1s4i04" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t14" form="i1s4i04">
+
-
 
+
-
<input name="id" type="hidden" value="i1s4i0" form="i1s4i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s4i05" form="i1s4i04"  
+
-
 
+
-
value="t14i1s4i0912.gif"> <input id="i1s4i06" type="submit"  
+
-
 
+
-
value="submit" form="i1s4i04" class="C1_button2" disabled=""> <div  
+
-
 
+
-
id="i1s4i03" class="M1_exp_img"></div> </td> <td  
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s4i01" class="C1_text">12th  
+
-
 
+
-
September
+
-
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
+
Bacteria:DH5a
Bacteria:DH5a
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
antibiotic putting in.
+
Total Obserbation Time:180mins
Total Obserbation Time:180mins
Analysis:  
Analysis:  
-
At the beginning, the population of cell was large enough to get valid fluorescence degree.  
+
At the beginning, the population of cell was large enough to get valid fluorescence degree. From then on ,there was  a weak decrease of the fluorescence degree.
-
 
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="0915  BL21(WT) gfp(pSB1C3)+aiiA(pSB3T5)" id="sb0s5"> <button class="M1_exp_step_name_button"><input value="0915  BL21(WT) gfp(pSB1C3)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/18 10:53:02--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s5i0">2013/9/25 20:50:35</td> <td class="M1_exp_item_content"> <table id="i0s5i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s5i00" src="http://trysomething-block.stor.sinaapp.com/t14i1s5i0915.gif"><br> <form id="i0s5i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s5i02" form="i0s5i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s5i04"> <input name="id" type="hidden" value="i0s5i0" form="i0s5i04"> <input name="oldgraph" type="hidden" id="i0s5i05" form="i0s5i04" value="t14i1s5i0915.gif"> <input id="i0s5i06" type="submit" value="submit" form="i0s5i04" class="C1_button2" disabled=""> <div id="i0s5i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s5i01" class="C1_text">15th September
-
From then on ,there was  a weak decrease of the fluorescence degee.
+
Plasmid:gfp(pSB1C3)+aiiA(pSB3T5)
-
</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="0915  BL21 Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s5">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="0915  BL21 Gfp(pSB1C3)  
+
-
 
+
-
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span  
+
-
 
+
-
class="M1_exp_step_time">2013/9/18 10:53:02--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s5i0">2013/9/25 20:50:35</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s5i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s5i00"
+
-
 
+
-
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s5i0915.gif"><br>
+
-
 
+
-
<form id="i1s5i04" target="M7" action="img.php" method="post"  
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"  
+
-
 
+
-
type="file" id="i1s5i02" form="i1s5i04" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t14" form="i1s5i04">
+
-
 
+
-
<input name="id" type="hidden" value="i1s5i0" form="i1s5i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s5i05" form="i1s5i04"  
+
-
 
+
-
value="t14i1s5i0915.gif"> <input id="i1s5i06" type="submit"  
+
-
 
+
-
value="submit" form="i1s5i04" class="C1_button2" disabled=""> <div  
+
-
 
+
-
id="i1s5i03" class="M1_exp_img"></div> </td> <td  
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s5i01" class="C1_text">15th  
+
-
 
+
-
September
+
-
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
+
Bacteria: BL21(WT)
Bacteria: BL21(WT)
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
antibiotic putting in.
+
Total Obserbation Time:515mins
Total Obserbation Time:515mins
Analysis:  
Analysis:  
-
From all the mircrofluid images, there is a tendency that the fluorescence degree change
+
From all the mircrofluid images, there was a tendency that the fluorescence degree changed from weak to strong and then changed from strong to weak.
-
 
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title=" 0918 BL21(WT) sfgfp(colE1)+aiiA(pSB3T5)" id="sb0s6"> <button class="M1_exp_step_name_button"><input value=" 0918 BL21(WT) sfgfp(colE1)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/18 10:53:24--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s6i0">2013/9/25 20:52:02</td> <td class="M1_exp_item_content"> <table id="i0s6i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s6i00" src="http://trysomething-block.stor.sinaapp.com/t14i1s6i0918.gif"><br> <form id="i0s6i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s6i02" form="i0s6i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s6i04"> <input name="id" type="hidden" value="i0s6i0" form="i0s6i04"> <input name="oldgraph" type="hidden" id="i0s6i05" form="i0s6i04" value="t14i1s6i0918.gif"> <input id="i0s6i06" type="submit" value="submit" form="i0s6i04" class="C1_button2" disabled=""> <div id="i0s6i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s6i01" class="C1_text">18th September
-
from weak to strong and then change from strong to weak.
+
Plasmid:sfgfp(colE1)+aiiA(pSB3T5)
-
</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title=" 0918 BL21(WT) sfGfp(coE1) aiia(pSB3T5)"  
+
-
 
+
-
id="sb1s6"> <button class="M1_exp_step_name_button"><input value=" 0918  
+
-
 
+
-
BL21(WT) sfGfp(coE1) aiia(pSB3T5)" class="M1_exp_step_name"></button>
+
-
 
+
-
<span class="M1_exp_step_time">2013/9/18 10:53:24--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s6i0">2013/9/25 20:52:02</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s6i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s6i00"
+
-
 
+
-
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s6i0918.gif"><br>
+
-
 
+
-
<form id="i1s6i04" target="M7" action="img.php" method="post"  
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"  
+
-
 
+
-
type="file" id="i1s6i02" form="i1s6i04" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t14" form="i1s6i04">
+
-
 
+
-
<input name="id" type="hidden" value="i1s6i0" form="i1s6i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s6i05" form="i1s6i04"  
+
-
 
+
-
value="t14i1s6i0918.gif"> <input id="i1s6i06" type="submit"  
+
-
 
+
-
value="submit" form="i1s6i04" class="C1_button2" disabled=""> <div  
+
-
 
+
-
id="i1s6i03" class="M1_exp_img"></div> </td> <td  
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s6i01" class="C1_text">18th  
+
-
 
+
-
September
+
-
Plasmid:sfGfp(coE1) aiia(pSB3T5)
+
Bacteria: BL21(WT)
Bacteria: BL21(WT)
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
antibiotic putting in.
+
Total Obserbation Time:590mins
Total Obserbation Time:590mins
Analysis:  
Analysis:  
-
There was a strong tendency that the fluorescence intensity came down, and it's the first  
+
There was a strong tendency that the fluorescence intensity came down, and it's the first time that we detected the most obvious changing.
-
 
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="0920 BL21(WT) sfgfp(colE1)+aiiA(pSB3T5)" id="sb0s7"> <button class="M1_exp_step_name_button"><input value="0920 BL21(WT) sfgfp(colE1)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/25 21:10:34--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s7i0">2013/9/25 21:11:06</td> <td class="M1_exp_item_content"> <table id="i0s7i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s7i00" src="http://trysomething-block.stor.sinaapp.com/t14i1s8i0920.gif"><br> <form id="i0s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s7i02" form="i0s7i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s7i04"> <input name="id" type="hidden" value="i0s7i0" form="i0s7i04"> <input name="oldgraph" type="hidden" id="i0s7i05" form="i0s7i04" value="t14i1s8i0920.gif"> <input id="i0s7i06" type="submit" value="submit" form="i0s7i04" class="C1_button2" disabled=""> <div id="i0s7i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s7i01" class="C1_text">20th September
-
time that we detected the most obvious changing.
+
Plasmid:sfgfp(colE1)+aiiA(pSB3T5)
-
</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="0920 BL21(WT) sfGfp(coE1) aiia(pSB3T5)"  
+
-
 
+
-
id="sb1s7"> <button class="M1_exp_step_name_button"><input value="0920  
+
-
 
+
-
BL21(WT) sfGfp(coE1) aiia(pSB3T5)" class="M1_exp_step_name"></button>
+
-
 
+
-
<span class="M1_exp_step_time">2013/9/25 21:10:34--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s7i0">2013/9/25 21:11:06</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s7i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s7i00"
+
-
 
+
-
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s8i0920.gif"><br>
+
-
 
+
-
<form id="i1s7i04" target="M7" action="img.php" method="post"  
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"  
+
-
 
+
-
type="file" id="i1s7i02" form="i1s7i04" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t14" form="i1s7i04">
+
-
 
+
-
<input name="id" type="hidden" value="i1s7i0" form="i1s7i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s7i05" form="i1s7i04"  
+
-
 
+
-
value="t14i1s8i0920.gif"> <input id="i1s7i06" type="submit"  
+
-
 
+
-
value="submit" form="i1s7i04" class="C1_button2" disabled=""> <div  
+
-
 
+
-
id="i1s7i03" class="M1_exp_img"></div> </td> <td  
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s7i01" class="C1_text">20th  
+
-
 
+
-
September
+
-
Plasmid:sfGfp(coE1) aiia(pSB3T5)
+
Bacteria: BL21(WT)
Bacteria: BL21(WT)
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.
-
 
+
Capturing time lapse:5mins
-
antibiotic putting in.
+
Total Obserbation Time:470mins
Total Obserbation Time:470mins
Analysis:  
Analysis:  
-
From all the microfluid images, there is a very weak decrease of the fluorescence degree.
+
From all the microfluid images, there was a very weak decrease of the fluorescence degree.
-
</textarea> <br> </td> </tr>
+
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="0922 BL21(WT) sfgfp(colE1)+aiiA(pSB3T5)" id="sb0s8"> <button class="M1_exp_step_name_button"><input value="0922 BL21(WT) sfgfp(colE1)+aiiA(pSB3T5)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/25 21:12:48--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i0">2013/9/25 21:13:01</td> <td class="M1_exp_item_content"> <table id="i0s8i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i00"  src="http://trysomething-block.stor.sinaapp.com/t14i0s8i04x.gif"><br> <form id="i0s8i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i02" form="i0s8i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s8i04"> <input name="id" type="hidden" value="i0s8i0" form="i0s8i04"> <input name="oldgraph" type="hidden" id="i0s8i05" form="i0s8i04" value="t14i0s8i04x.gif"> <input id="i0s8i06" type="submit" value="submit" form="i0s8i04" class="C1_button2" disabled=""> <div id="i0s8i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i01" class="C1_text">4X
 +
</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i1">2013/9/28 07:14:25</td> <td class="M1_exp_item_content"> <table id="i0s8i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i10"  src="http://trysomething-block.stor.sinaapp.com/t14i0s8i110x.gif"><br> <form id="i0s8i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i12" form="i0s8i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s8i14"> <input name="id" type="hidden" value="i0s8i1" form="i0s8i14"> <input name="oldgraph" type="hidden" id="i0s8i15" form="i0s8i14" value="t14i0s8i110x.gif"> <input id="i0s8i16" type="submit" value="submit" form="i0s8i14" class="C1_button2" disabled=""> <div id="i0s8i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i11" class="C1_text">10X</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i2">2013/9/28 07:14:27</td> <td class="M1_exp_item_content"> <table id="i0s8i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i20"  src="http://trysomething-block.stor.sinaapp.com/t14i0s8i220x1.gif"><br> <form id="i0s8i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i22" form="i0s8i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s8i24"> <input name="id" type="hidden" value="i0s8i2" form="i0s8i24"> <input name="oldgraph" type="hidden" id="i0s8i25" form="i0s8i24" value="t14i0s8i220x1.gif"> <input id="i0s8i26" type="submit" value="submit" form="i0s8i24" class="C1_button2" disabled=""> <div id="i0s8i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i21" class="C1_text">20X(light field)</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i3">2013/9/28 07:14:28</td> <td class="M1_exp_item_content"> <table id="i0s8i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i30"  src="http://trysomething-block.stor.sinaapp.com/t14i0s8i320x2.gif"><br> <form id="i0s8i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i32" form="i0s8i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s8i34"> <input name="id" type="hidden" value="i0s8i3" form="i0s8i34"> <input name="oldgraph" type="hidden" id="i0s8i35" form="i0s8i34" value="t14i0s8i320x2.gif"> <input id="i0s8i36" type="submit" value="submit" form="i0s8i34" class="C1_button2" disabled=""> <div id="i0s8i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i31" class="C1_text">20X
-
</tbody></table> </td> </tr> </tbody></table>
+
22th September
-
 
+
Plasmid:sfgfp(colE1)+aiiA(pSB3T5)
-
<div class="M1_exp_stepInfo" title="0922 BL21(WT) sfGfp(coE1) aiia(pSB3T5)"
+
-
 
+
-
id="sb1s8"> <button class="M1_exp_step_name_button"><input value="0922
+
-
 
+
-
BL21(WT) sfGfp(coE1) aiia(pSB3T5)" class="M1_exp_step_name"></button>
+
-
 
+
-
<span class="M1_exp_step_time">2013/9/25 21:12:48--Chun Tang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s8i0">2013/9/25 21:13:01</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s8i0" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s8i00"
+
-
 
+
-
class="C1_graph" src="http://1.trysomething.sinaapp.com/M.php"><br>
+
-
 
+
-
<form id="i1s8i04" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s8i02"
+
-
 
+
-
form="i1s8i04" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t14" form="i1s8i04">
+
-
 
+
-
<input name="id" type="hidden" value="i1s8i0" form="i1s8i04">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s8i05" form="i1s8i04" value="">
+
-
 
+
-
<input id="i1s8i06" type="submit" value="submit" form="i1s8i04" class="C1_button2"
+
-
 
+
-
disabled=""> <div id="i1s8i03" class="M1_exp_img"></div>
+
-
 
+
-
</td> <td class="C1_td_text">
+
-
 
+
-
<textarea id="i1s8i01" class="C1_text">22th September
+
-
Plasmid:sfGfp(coE1) aiia(pSB3T5)
+
Bacteria: BL21(WT)
Bacteria: BL21(WT)
-
Condition: Flowing medium culture supply. Cells could flow into different traps. With  
+
Condition: Flowing medium culture supply. Cells could flow into different traps. With antibiotic putting in.Changing different lens magnification
-
 
+
Total Obserbation Time:600mins
-
antibiotic putting in.
+
Observe time interval:20mins
-
Total Obserbation Time:?
+
Analysis:  
Analysis:  
-
?
+
There was a tendency that the fluorescence degree changed from weak to strong and then changed from strong to weak.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="Microfluidic" id="sb0s9"> <button class="M1_exp_step_name_button"><input value="Microfluidic" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/25 21:15:00--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s9i0">2013/9/25 21:15:47</td> <td class="M1_exp_item_content"> <table id="i0s9i0" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s9i00"  src="http://trysomething-block.stor.sinaapp.com/t14i1s10i0DSC_0455.JPG" height=auto
-
</textarea> <br> </td> </tr>
+
width=562px><br> <form id="i0s9i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s9i02" form="i0s9i04" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s9i04"> <input name="id" type="hidden" value="i0s9i0" form="i0s9i04"> <input name="oldgraph" type="hidden" id="i0s9i05" form="i0s9i04" value="t14i1s10i0DSC_0455.JPG"> <input id="i0s9i06" type="submit" value="submit" form="i0s9i04" class="C1_button2" disabled=""> <div id="i0s9i03" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s9i01" class="C1_text">Microscope</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s9i1">2013/9/25 21:16:23</td> <td class="M1_exp_item_content"> <table id="i0s9i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s9i10"  src="http://trysomething-block.stor.sinaapp.com/t14i1s10i1wi-part2.jpg"><br> <form id="i0s9i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s9i12" form="i0s9i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s9i14"> <input name="id" type="hidden" value="i0s9i1" form="i0s9i14"> <input name="oldgraph" type="hidden" id="i0s9i15" form="i0s9i14" value="t14i1s10i1wi-part2.jpg"> <input id="i0s9i16" type="submit" value="submit" form="i0s9i14" class="C1_button2" disabled=""> <div id="i0s9i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s9i11" class="C1_text">Microfluidic chip</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s9i2">2013/9/25 21:16:51</td> <td class="M1_exp_item_content"> <table id="i0s9i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s9i20"  src="http://trysomething-block.stor.sinaapp.com/t14i1s10i2wl-part.jpg"><br> <form id="i0s9i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s9i22" form="i0s9i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i0s9i24"> <input name="id" type="hidden" value="i0s9i2" form="i0s9i24"> <input name="oldgraph" type="hidden" id="i0s9i25" form="i0s9i24" value="t14i1s10i2wl-part.jpg"> <input id="i0s9i26" type="submit" value="submit" form="i0s9i24" class="C1_button2" disabled=""> <div id="i0s9i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s9i21" class="C1_text">Valves</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> </div>
-
</tbody></table> </td> </tr> </tbody></table>
 
-
<div class="M1_exp_stepInfo" title="Microfluidic" id="sb1s9">
 
-
<button class="M1_exp_step_name_button"><input value="Microfluidic"  
+
<div class="microfluidic">
 +
<h1>RFU (9.24-9.27)</h1>
 +
<div class="M1_exp_stepInfo" title="RFU (9.24-9.27)" id="sb8s0"> <button class="M1_exp_step_name_button"><input value="RFU (9.24-9.27)" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/27 21:59:07--Chun Tang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib8s0i0">2013/9/27 21:59:17</td> <td class="M1_exp_item_content"><textarea id="i8s0i0" class="C0" rows="1">  As we have made BL21(WT) with several plasmids, it was necessary to determine the OD and fluorescence value of the bacteria so that we could know if BL21(WT) with the plasmids was able to oscillate. To achieve the goal, we used enzyme-labeled instrument. By sampling and measuring on time, we could see that if our circuit would work smoothly.
 +
  After sampling per hour, we centrifuged samples at 6,000g for 10min. And then we discarded flow-through liquid. Next, we added PBS buffer into bacteria. Using enzyme-labeled instrument, we got a serious of data of OD and fluorescence value. We did it three times.</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib8s0i1">2013/9/28 03:11:44</td> <td class="M1_exp_item_content"> <table id="i8s0i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i8s0i10"  src="http://trysomething-block.stor.sinaapp.com/t14i8s0i12.jpg"><br> <form id="i8s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i8s0i12" form="i8s0i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i8s0i14"> <input name="id" type="hidden" value="i8s0i1" form="i8s0i14"> <input name="oldgraph" type="hidden" id="i8s0i15" form="i8s0i14" value="t14i8s0i12.jpg"> <input id="i8s0i16" type="submit" value="submit" form="i8s0i14" class="C1_button2" disabled=""> <div id="i8s0i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i8s0i11" class="C1_text">The first time we measured the fluorescence value.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib8s0i2">2013/9/28 03:30:57</td> <td class="M1_exp_item_content"> <table id="i8s0i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i8s0i20"  src="http://trysomething-block.stor.sinaapp.com/t14i8s0i23.jpg"><br> <form id="i8s0i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i8s0i22" form="i8s0i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i8s0i24"> <input name="id" type="hidden" value="i8s0i2" form="i8s0i24"> <input name="oldgraph" type="hidden" id="i8s0i25" form="i8s0i24" value="t14i8s0i23.jpg"> <input id="i8s0i26" type="submit" value="submit" form="i8s0i24" class="C1_button2" disabled=""> <div id="i8s0i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i8s0i21" class="C1_text">The second time we measured the fluorescence value.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib8s0i3">2013/9/28 03:31:38</td> <td class="M1_exp_item_content"> <table id="i8s0i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i8s0i30"  src="http://trysomething-block.stor.sinaapp.com/t14i8s0i34.jpg"><br> <form id="i8s0i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i8s0i32" form="i8s0i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i8s0i34"> <input name="id" type="hidden" value="i8s0i3" form="i8s0i34"> <input name="oldgraph" type="hidden" id="i8s0i35" form="i8s0i34" value="t14i8s0i34.jpg"> <input id="i8s0i36" type="submit" value="submit" form="i8s0i34" class="C1_button2" disabled=""> <div id="i8s0i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i8s0i31" class="C1_text">The second time we measured the OD.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib8s0i4">2013/9/28 03:34:06</td> <td class="M1_exp_item_content"> <table id="i8s0i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i8s0i40"  src="http://trysomething-block.stor.sinaapp.com/t14i8s0i51.jpg"><br> <form id="i8s0i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i8s0i42" form="i8s0i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t14" form="i8s0i44"> <input name="id" type="hidden" value="i8s0i4" form="i8s0i44"> <input name="oldgraph" type="hidden" id="i8s0i45" form="i8s0i44" value="t14i8s0i51.jpg"> <input id="i8s0i46" type="submit" value="submit" form="i8s0i44" class="C1_button2" disabled=""> <div id="i8s0i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i8s0i41" class="C1_text">The third time we measured the fluorescence value.</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> </div>
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class="M1_exp_step_name"></button> <span
 
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<td class="M1_exp_item_time" id="ib1s9i0">2013/9/25 21:15:47</td>
 
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Latest revision as of 03:09, 28 September 2013

导航栏

Biobrick Transformation (6.24-6.30)

2013/9/26 20:16:17--Chun Tang
2013/9/26 20:16:39
2013/9/26 20:17:26


Plasmids Construction (7.1-7.28, 8.15-9.16)

2013/9/26 20:36:39--Chun Tang
2013/9/26 20:36:58
2013/9/28 01:03:53


2013/9/26 20:37:51--Chun Tang
2013/9/26 20:38:17
2013/9/28 01:05:10


2013/9/28 01:05:15


2013/9/26 20:40:10--Chun Tang
2013/9/26 20:40:27
2013/9/28 01:06:41


2013/9/28 01:06:51


2013/9/26 20:43:04--Chun Tang
2013/9/26 20:43:20
2013/9/28 01:10:10


2013/9/28 01:10:38


2013/9/26 20:44:56--Chun Tang
2013/9/26 20:45:14
2013/9/28 01:11:54


2013/9/28 01:12:42


2013/9/26 20:47:01--Chun Tang
2013/9/26 20:47:15
2013/9/28 01:16:48


2013/9/28 01:17:27


2013/9/26 20:48:37--Chun Tang
2013/9/26 20:48:50
2013/9/28 01:19:02


Transformation (7.29-9.20)

2013/9/26 20:49:56--Chun Tang
2013/9/26 20:50:04
2013/9/28 01:21:56


2013/9/28 01:22:37


2013/9/26 20:51:34--Chun Tang
2013/9/26 20:51:47
2013/9/28 01:23:53


2013/9/26 20:52:47--Chun Tang
2013/9/26 20:53:01
2013/9/28 01:24:59


2013/9/26 20:55:54--Chun Tang
2013/9/26 20:56:19

SDS-PAGE

2013/9/26 21:48:57--Tang Chun
2013/9/26 21:49:44
2013/9/26 21:50:08--Tang Chun
2013/9/26 21:51:07
2013/9/26 21:53:17--Tang Chun
2013/9/26 21:55:45
2013/9/26 22:02:08--Tang Chun
2013/9/26 22:03:34


2013/9/26 22:04:18--Tang Chun
2013/9/26 22:07:07


2013/9/26 22:07:33--Tang Chun
2013/9/26 22:08:04
2013/9/26 22:07:46


2013/9/26 22:08:31--Tang Chun
2013/9/26 22:08:47


2013/9/26 22:09:10--Tang Chun
2013/9/26 22:09:28


2013/9/26 22:09:40


2013/9/26 22:10:13--Tang Chun
2013/9/26 22:10:54


2013/9/26 22:11:28


2013/9/26 22:12:08--Tang Chun
2013/9/26 22:12:23


2013/9/26 22:12:32


2013/9/26 22:13:05--Tang Chun
2013/9/26 22:13:16


2013/9/28 07:03:20--Chun Tang
2013/9/28 07:03:54


2013/9/28 08:44:02--Chun Tang
2013/9/28 08:44:22


2013/9/28 08:47:11


2013/9/28 08:44:24


2013/9/28 08:44:24


2013/9/28 08:44:28


Microfluidic Experiment Record

2013/9/18 10:50:50--Chun Tang
2013/9/25 20:38:47


2013/9/18 10:51:01--Chun Tang
2013/9/25 20:42:38


2013/9/18 10:51:41--Chun Tang
2013/9/25 20:45:15


2013/9/18 10:52:00--Chun Tang
2013/9/25 20:46:04


2013/9/18 10:52:31--Chun Tang
2013/9/25 20:47:42


2013/9/18 10:53:02--Chun Tang
2013/9/25 20:50:35


2013/9/18 10:53:24--Chun Tang
2013/9/25 20:52:02


2013/9/25 21:10:34--Chun Tang
2013/9/25 21:11:06


2013/9/25 21:12:48--Chun Tang
2013/9/25 21:13:01


2013/9/28 07:14:25


2013/9/28 07:14:27


2013/9/28 07:14:28


2013/9/25 21:15:00--Chun Tang
2013/9/25 21:15:47


2013/9/25 21:16:23


2013/9/25 21:16:51


RFU (9.24-9.27)

2013/9/27 21:59:07--Chun Tang
2013/9/27 21:59:17
2013/9/28 03:11:44


2013/9/28 03:30:57


2013/9/28 03:31:38


2013/9/28 03:34:06



Retrieved from "http://2013.igem.org/Team:XMU-China/wetlab_journal"