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Latest revision as of 03:09, 28 September 2013

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Biobrick Transformation (6.24-6.30)

2013/9/26 20:16:17--Chun Tang

2013/9/26 20:16:39

2013/9/26 20:17:26

Plasmids Construction (7.1-7.28, 8.15-9.16)

2013/9/26 20:36:39--Chun Tang

2013/9/26 20:36:58

2013/9/28 01:03:53

2013/9/26 20:37:51--Chun Tang

2013/9/26 20:38:17

2013/9/28 01:05:10

2013/9/28 01:05:15

2013/9/26 20:40:10--Chun Tang

2013/9/26 20:40:27

2013/9/28 01:06:41

2013/9/28 01:06:51

2013/9/26 20:43:04--Chun Tang

2013/9/26 20:43:20

2013/9/28 01:10:10

2013/9/28 01:10:38

2013/9/26 20:44:56--Chun Tang

2013/9/26 20:45:14

2013/9/28 01:11:54

2013/9/28 01:12:42

2013/9/26 20:47:01--Chun Tang

2013/9/26 20:47:15

2013/9/28 01:16:48

2013/9/28 01:17:27

2013/9/26 20:48:37--Chun Tang

2013/9/26 20:48:50

2013/9/28 01:19:02

Transformation (7.29-9.20)

2013/9/26 20:49:56--Chun Tang

2013/9/26 20:50:04

2013/9/28 01:21:56

2013/9/28 01:22:37

2013/9/26 20:51:34--Chun Tang

2013/9/26 20:51:47

2013/9/28 01:23:53

2013/9/26 20:52:47--Chun Tang

2013/9/26 20:53:01

2013/9/28 01:24:59

2013/9/26 20:55:54--Chun Tang

2013/9/26 20:56:19

SDS-PAGE

2013/9/26 21:48:57--Tang Chun

2013/9/26 21:49:44

2013/9/26 21:50:08--Tang Chun

2013/9/26 21:51:07

2013/9/26 21:53:17--Tang Chun

2013/9/26 21:55:45

2013/9/26 22:02:08--Tang Chun

2013/9/26 22:03:34

2013/9/26 22:04:18--Tang Chun

2013/9/26 22:07:07

2013/9/26 22:07:33--Tang Chun

2013/9/26 22:08:04

2013/9/26 22:07:46

2013/9/26 22:08:31--Tang Chun

2013/9/26 22:08:47

2013/9/26 22:09:10--Tang Chun

2013/9/26 22:09:28

2013/9/26 22:09:40

2013/9/26 22:10:13--Tang Chun

2013/9/26 22:10:54

2013/9/26 22:11:28

2013/9/26 22:12:08--Tang Chun

2013/9/26 22:12:23

2013/9/26 22:12:32

2013/9/26 22:13:05--Tang Chun

2013/9/26 22:13:16

2013/9/28 07:03:20--Chun Tang

2013/9/28 07:03:54

2013/9/28 08:44:02--Chun Tang

2013/9/28 08:44:22

2013/9/28 08:47:11

2013/9/28 08:44:24

2013/9/28 08:44:28

Microfluidic Experiment Record

2013/9/18 10:50:50--Chun Tang

2013/9/25 20:38:47

2013/9/18 10:51:01--Chun Tang

2013/9/25 20:42:38

2013/9/18 10:51:41--Chun Tang

2013/9/25 20:45:15

2013/9/18 10:52:00--Chun Tang

2013/9/25 20:46:04

2013/9/18 10:52:31--Chun Tang

2013/9/25 20:47:42

2013/9/18 10:53:02--Chun Tang

2013/9/25 20:50:35

2013/9/18 10:53:24--Chun Tang

2013/9/25 20:52:02

2013/9/25 21:10:34--Chun Tang

2013/9/25 21:11:06

2013/9/25 21:12:48--Chun Tang

2013/9/25 21:13:01

2013/9/28 07:14:25

2013/9/28 07:14:27

2013/9/28 07:14:28

2013/9/25 21:15:00--Chun Tang

2013/9/25 21:15:47

2013/9/25 21:16:23

2013/9/25 21:16:51

RFU (9.24-9.27)

2013/9/27 21:59:07--Chun Tang

2013/9/27 21:59:17

2013/9/28 03:11:44

2013/9/28 03:30:57

2013/9/28 03:31:38

2013/9/28 03:34:06

@@ Line 127: / Line 127: @@
 <div class="microfluidic">
 <h1>Transformation (7.29-9.20)</h1>
-		<div class="M1_exp_stepInfo" title="BL21 (DE3) (7.29-8.15)" id="sb6s0">			<button class="M1_exp_step_name_button"><input value="BL21 (DE3) (7.29-8.15)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 20:49:56--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:50:04</td>			<td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1">  Because we had constructed three plasmids we need, our next goal was to transform the plasmids into BL21(DE3). We first constructed a double-plasmid BL21: GFP aiiA, and then we transformed the third plasmid into BL21 with two plasmids.</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s0i1">2013/9/28 01:21:56</td>			<td class="M1_exp_item_content">	<table id="i6s0i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i6s0i10" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i6s0i3图片14.jpg"><br>				<form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i6s0i14">				<input name="id" type="hidden" value="i6s0i1" form="i6s0i14">				<input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i3图片14.jpg">				<input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled="">				<div id="i6s0i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i6s0i11" class="C1_text">M:Marker  1kb
+		<div class="M1_exp_stepInfo" title="BL21 (DE3) (7.29-8.15)" id="sb6s0">			<button class="M1_exp_step_name_button"><input value="BL21 (DE3) (7.29-8.15)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 20:49:56--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s0i0">2013/9/26 20:50:04</td>			<td class="M1_exp_item_content"><textarea id="i6s0i0" class="C0" rows="1">  Because we had constructed three plasmids we need, our next goal was to transform the plasmids into BL21(DE3). We first constructed a double-plasmid BL21: GFP+aiiA, and then we transformed the third plasmid into BL21 with two plasmids.</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s0i1">2013/9/28 01:21:56</td>			<td class="M1_exp_item_content">	<table id="i6s0i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i6s0i10" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i6s0i3图片14.jpg"><br>				<form id="i6s0i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i6s0i12" form="i6s0i14" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i6s0i14">				<input name="id" type="hidden" value="i6s0i1" form="i6s0i14">				<input name="oldgraph" type="hidden" id="i6s0i15" form="i6s0i14" value="t14i6s0i3图片14.jpg">				<input id="i6s0i16" type="submit" value="submit" form="i6s0i14" class="C1_button2" disabled="">				<div id="i6s0i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i6s0i11" class="C1_text">M:Marker  1kb
 Lane1:GFP plasmid,digestion of EcoRI and SpeI
 Lane2:GFP plasmid,digestion of EcoRI
@@ Line 137: / Line 137: @@
 Lane8:ndh plasmid,digestion of EcoRI
 Lane9:ndh plasmid
-Lane10,15,16:aiiA GFP plasmid
+Lane10,15,16:aiiA+GFP plasmid
-Lane11,14,17:aiiA GFP plasmid,digestion of EcoRI
+Lane11,14,17:aiiA+GFP plasmid,digestion of EcoRI
-Lane12,13,18:aiiA GFP plasmid ,digestion of EcoRI and SpeI
+Lane12,13,18:aiiA+GFP plasmid ,digestion of EcoRI and SpeI
 </textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s0i2">2013/9/28 01:22:37</td>			<td class="M1_exp_item_content">	<table id="i6s0i2" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i6s0i20"  src="http://trysomething-block.stor.sinaapp.com/t14i6s0i4图片15.jpg"><br>				<form id="i6s0i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i6s0i22" form="i6s0i24" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i6s0i24">				<input name="id" type="hidden" value="i6s0i2" form="i6s0i24">				<input name="oldgraph" type="hidden" id="i6s0i25" form="i6s0i24" value="t14i6s0i4图片15.jpg">				<input id="i6s0i26" type="submit" value="submit" form="i6s0i24" class="C1_button2" disabled="">				<div id="i6s0i23" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i6s0i21" class="C1_text">M：Marker 1kb
-Lane1: plasmids (GFP aiiA)
+Lane1: plasmids (GFP+aiiA)
 Lane2: three plasmids (GFP, aiiA, ndh)
 </textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title=" DH5α (7.30-9.20)" id="sb6s1">			<button class="M1_exp_step_name_button"><input value=" DH5α (7.30-9.20)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 20:51:34--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s1i0">2013/9/26 20:51:47</td>			<td class="M1_exp_item_content"><textarea id="i6s1i0" class="C0" rows="1">  Although we have transformed three plasmids into BL21, the result was undesirable. We found that DH5α may be more suitable for our vector, so we decided to transform the plasmids into DH5α one more time.
-   Later we constructed 1A3 GFP, colE1 GFP, sfGFP, colE1 sfGFP, and aiiA/ndh pSB3T5, so we transformed all these plasmids into DH5α for our new work of construction.
+   Later we constructed 1A3 GFP, colE1＋GFP, sfGFP, colE1＋sfGFP, and aiiA/ndh pSB3T5, so we transformed all these plasmids into DH5α for our new work of construction.
 </textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s1i1">2013/9/28 01:23:53</td>			<td class="M1_exp_item_content">	<table id="i6s1i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i6s1i10"  src="http://trysomething-block.stor.sinaapp.com/t14i6s1i2图片16.jpg"><br>				<form id="i6s1i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i6s1i12" form="i6s1i14" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i6s1i14">				<input name="id" type="hidden" value="i6s1i1" form="i6s1i14">				<input name="oldgraph" type="hidden" id="i6s1i15" form="i6s1i14" value="t14i6s1i2图片16.jpg">				<input id="i6s1i16" type="submit" value="submit" form="i6s1i14" class="C1_button2" disabled="">				<div id="i6s1i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i6s1i11" class="C1_text">M: Marker 1kb
-Lane1,6,7: GFP aiiA ndh, digestion of EcoRI and PstI
+Lane1,6,7: GFP+aiiA ndh, digestion of EcoRI and PstI
-Lane2,5,8: GFP aiiA ndh, digestion of EcoRI
+Lane2,5,8: GFP+aiiA ndh, digestion of EcoRI
-Lane3,4,9: GFP aiiA ndh plasmids
+Lane3,4,9: GFP+aiiA+ndh plasmids
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="BL21 (WT) (9.11-9.20)" id="sb6s2">			<button class="M1_exp_step_name_button"><input value="BL21 (WT) (9.11-9.20)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 20:52:47--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s2i0">2013/9/26 20:53:01</td>			<td class="M1_exp_item_content"><textarea id="i6s2i0" class="C0" rows="1">  Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed aiiA/ndh pSB3T5, sfGFP, colE1 GFP, colE1 sfGFP and GFP pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s2i1">2013/9/28 01:24:59</td>			<td class="M1_exp_item_content">	<table id="i6s2i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i6s2i10" width="450px" height="auto"  src="http://trysomething-block.stor.sinaapp.com/t14i6s2i2图片17.jpg"><br>				<form id="i6s2i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i6s2i12" form="i6s2i14" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i6s2i14">				<input name="id" type="hidden" value="i6s2i1" form="i6s2i14">				<input name="oldgraph" type="hidden" id="i6s2i15" form="i6s2i14" value="t14i6s2i2图片17.jpg">				<input id="i6s2i16" type="submit" value="submit" form="i6s2i14" class="C1_button2" disabled="">				<div id="i6s2i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i6s2i11" class="C1_text">M:Marker 1kb
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="BL21 (WT) (9.11-9.20)" id="sb6s2">			<button class="M1_exp_step_name_button"><input value="BL21 (WT) (9.11-9.20)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 20:52:47--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s2i0">2013/9/26 20:53:01</td>			<td class="M1_exp_item_content"><textarea id="i6s2i0" class="C0" rows="1">  Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed aiiA/ndh pSB3T5, sfGFP, colE1＋GFP, colE1＋sfGFP and GFP＋pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s2i1">2013/9/28 01:24:59</td>			<td class="M1_exp_item_content">	<table id="i6s2i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i6s2i10" width="450px" height="auto"  src="http://trysomething-block.stor.sinaapp.com/t14i6s2i2图片17.jpg"><br>				<form id="i6s2i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i6s2i12" form="i6s2i14" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i6s2i14">				<input name="id" type="hidden" value="i6s2i1" form="i6s2i14">				<input name="oldgraph" type="hidden" id="i6s2i15" form="i6s2i14" value="t14i6s2i2图片17.jpg">				<input id="i6s2i16" type="submit" value="submit" form="i6s2i14" class="C1_button2" disabled="">				<div id="i6s2i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i6s2i11" class="C1_text">M:Marker 1kb
-Lane1,4 :GFP(1A3) aiiA ndh plasmid
+Lane1,4 :GFP(1A3)＋aiiA+ndh plasmid
-Lane2,5 : GFP(1A3) aiiA ndh plasmid,digestion of XbaI
+Lane2,5 : GFP(1A3)＋aiiA+ndh plasmid,digestion of XbaI
-Lane3,6 : GFP(1A3) aiiA ndh plasmid,digestion of XbaI and PstI
+Lane3,6 : GFP(1A3)＋aiiA+ndh plasmid,digestion of XbaI and PstI
-Lane7: GFP(3H) aiiA ndh plasmid
+Lane7: GFP(3H) aiiA+ndh plasmid
-Lane8: GFP(3H) aiiA ndh plasmid digestion of XbaI
+Lane8: GFP(3H) aiiA+ndh plasmid digestion of XbaI
-Lane9: GFP(3H) aiiA ndh plasmid digestion of XbaI and PstI
+Lane9: GFP(3H) aiiA+ndh plasmid digestion of XbaI and PstI
-Lane10: sfGFP(1C3) aiiA ndh plasmid
+Lane10: sfGFP(1C3)＋aiiA+ndh plasmid
-Lane11: sfGFP(1C3) aiiA ndh plasmid digestion of XbaI
+Lane11: sfGFP(1C3)＋aiiA+ndh plasmid digestion of XbaI
-Lane12: sfGFP(1C3) aiiA ndh plasmid digestion of XbaI and PstI
+Lane12: sfGFP(1C3)＋aiiA+ndh plasmid digestion of XbaI and PstI
-Lane13: sfGFP(3H) aiiA ndh plasmid
+Lane13: sfGFP(3H)＋aiiA+ndh plasmid
-Lane14: sfGFP(3H) aiiA ndh plasmid digestion of XbaI
+Lane14: sfGFP(3H)＋aiiA+ndh plasmid digestion of XbaI
-Lane15: sfGFP(3H) aiiA ndh plasmid digestion of XbaI and PstI
+Lane15: sfGFP(3H)＋aiiA+ndh plasmid digestion of XbaI and PstI
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="MG1655 (9.11-9.21)" id="sb6s3">			<button class="M1_exp_step_name_button"><input value="MG1655 (9.11-9.21)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 20:55:54--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s3i0">2013/9/26 20:56:19</td>			<td class="M1_exp_item_content"><textarea id="i6s3i0" class="C0" rows="1">  Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed sfGFP, colE1 GFP, and colE1 sfGFP, GFP pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td>		</tr>		</tbody></table>		</div>
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="MG1655 (9.11-9.21)" id="sb6s3">			<button class="M1_exp_step_name_button"><input value="MG1655 (9.11-9.21)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 20:55:54--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib6s3i0">2013/9/26 20:56:19</td>			<td class="M1_exp_item_content"><textarea id="i6s3i0" class="C0" rows="1">  Considered the failure of conveying in BL21, we used other bacteria for our vector. In addition, this time we transformed sfGFP, colE1＋GFP, and colE1＋sfGFP, GFP＋pSB1A3 besides other plasmid we had transformed into BL21.</textarea></td>		</tr>		</tbody></table>		</div>
 <div class="microfluidic">
@@ Line 172: / Line 172: @@
 Data analysis:
    The band marked in red disappeared is pSB1C3-gfp-luxI/BL21.The LuxR, GFP and AiiA  are the same of size (marked in yellow). However the band of AiiA was not clear. Many target proteins were misfolded. Therefore the culture condition should be optimized.
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="3. (8.12)" id="sb7s5" >			<button class="M1_exp_step_name_button" ><input value="3. (8.12)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:07:33--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i0">2013/9/26 22:08:04</td>			<td class="M1_exp_item_content"><textarea id="i7s5i0" class="C0" rows="1" disabled="" style="background-color: white;"> Characterization of expression of E.coli transformed into triple plasmids and testing if our target proteins expressed by our constructed gene circuits were concentrated in precipitation or supernatant  (8.12)</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i1">2013/9/26 22:07:46</td>			<td class="M1_exp_item_content">	<table id="i7s5i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s5i10" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s5i03.png"><br>				<form id="i7s5i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s5i12" form="i7s5i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s5i14">				<input name="id" type="hidden" value="i7s5i1" form="i7s5i14">				<input name="oldgraph" type="hidden" id="i7s5i15" form="i7s5i14" value="t14i9s5i03.png">				<input id="i7s5i16" type="submit" value="submit" form="i7s5i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s5i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s5i11" class="C1_text" disabled="" style="background-color: white;">Description :
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="3. (8.12)" id="sb7s5" >			<button class="M1_exp_step_name_button" ><input value="3. (8.12)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:07:33--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i0">2013/9/26 22:08:04</td>			<td class="M1_exp_item_content"><textarea id="i7s5i0" class="C0" rows="1" disabled="" style="background-color: white;"> Characterization of expression of E.coli transformed into triple plasmids and testing if our target proteins expressed by our constructed gene circuits were concentrated in precipitation or supernatant  (8.12)</textarea></td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s5i1">2013/9/26 22:07:46</td>			<td class="M1_exp_item_content">	<table id="i7s5i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s5i10" width="600" src="http://trysomething-block.stor.sinaapp.com/t14i9s5i03.png"><br>				<form id="i7s5i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s5i12" form="i7s5i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s5i14">				<input name="id" type="hidden" value="i7s5i1" form="i7s5i14">				<input name="oldgraph" type="hidden" id="i7s5i15" form="i7s5i14" value="t14i9s5i03.png">				<input id="i7s5i16" type="submit" value="submit" form="i7s5i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s5i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s5i11" class="C1_text" disabled="" style="background-color: white;">Description :
   We had transformed triple plasmids into one E.coli and the DNA gel picture indicated it was ligated successfully. So now we were very eager to know if our circuits could work well in E.coli.
 Furthermore, we wanted to know that if our target proteins expressed by our constructed gene circuits, in other words, pSB1C3-gfp-luxI and pSB13T5-aiiA were aggregated in precipitation or supernatant. So we run SDS-PAGE using BL21(blank control), three cells transformed into different single plasmids(aiiA, ndh and gfp), one cell transformed into double plasmids(aiiA and gfp) and our E.coli transformed into triple plasmids in three forms respectively, whole cell, precipitation and supernatant.
@@ Line 182: / Line 182: @@
 Data analysis:
 The band marked in red was not the target band. Maybe it is the complex of AHL-LuxI. The target proteins of pSB1C3-gfp-luxI have been expressed while the target proteins of pSB3T5-aiiA and pSB4K5-ndh did not be  expressed because the promoter should be induced by AHL.
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" >			<button class="M1_exp_step_name_button" ><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td>			<td class="M1_exp_item_content">	<table id="i7s7i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br>				<form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s7i04">				<input name="id" type="hidden" value="i7s7i0" form="i7s7i04">				<input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png">				<input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s7i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;">  We were suggested that DH5α has a better viability and a higher protein expression. So we maybe there's some mistakes in our strains which cause our couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="5. Confirmation of the expression level cultured at 30℃(8.31)" id="sb7s7" >			<button class="M1_exp_step_name_button" ><input value="5. Confirmation of the expression level cultured at 30℃(8.31)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/26 22:09:10--Tang Chun</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s7i0">2013/9/26 22:09:28</td>			<td class="M1_exp_item_content">	<table id="i7s7i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s7i00" width="500px" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i05.png"><br>				<form id="i7s7i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s7i02" form="i7s7i04" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s7i04">				<input name="id" type="hidden" value="i7s7i0" form="i7s7i04">				<input name="oldgraph" type="hidden" id="i7s7i05" form="i7s7i04" value="t14i9s7i05.png">				<input id="i7s7i06" type="submit" value="submit" form="i7s7i04" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s7i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s7i01" class="C1_text" disabled="" style="background-color: white;">  We were suggested that DH5α has a better viability and a higher protein expression. So  there's some mistakes in our strains which cause us couldn't get a expected microfluidic experiment result. So we compared two identical groups cultured at different degrees, 30℃ and 37℃.
 Data analysis:
 E.coli maybe cultured at 30℃ had a stronger expression of protein from picture 1 but we were confused by picture 2 cause it indicated that expression at 30℃ is not larger than expression at 37℃.</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>				<tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s7i1">2013/9/26 22:09:40</td>			<td class="M1_exp_item_content">	<table id="i7s7i1" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s7i10" src="http://trysomething-block.stor.sinaapp.com/t14i9s7i16.png"><br>				<form id="i7s7i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s7i12" form="i7s7i14" class="C1_button1" required="required" disabled="" style="background-color: white;">				<input name="pname" type="hidden" value="t14" form="i7s7i14">				<input name="id" type="hidden" value="i7s7i1" form="i7s7i14">				<input name="oldgraph" type="hidden" id="i7s7i15" form="i7s7i14" value="t14i9s7i16.png">				<input id="i7s7i16" type="submit" value="submit" form="i7s7i14" class="C1_button2" disabled="" style="background-color: white;">				<div id="i7s7i13" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s7i11" class="C1_text" disabled="" style="background-color: white;">Future plan:
@@ Line 213: / Line 213: @@
 There is a difference between N and N+A：
 The band marked in green/red shows that  E.coli cultured has a stronger expression of protein when  added AHL?
-</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="10.Characterization of several parts using additional AHL .(9.27)" id="sb7s12">			<button class="M1_exp_step_name_button"><input value="10.Characterization of several parts using additional AHL .(9.27)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/28 08:44:02--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s12i0">2013/9/28 08:44:22</td>			<td class="M1_exp_item_content">	<table id="i7s12i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s12i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i0sds1.png"><br>				<form id="i7s12i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s12i02" form="i7s12i04" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i7s12i04">				<input name="id" type="hidden" value="i7s12i0" form="i7s12i04">				<input name="oldgraph" type="hidden" id="i7s12i05" form="i7s12i04" value="t14i7s12i0sds1.png">				<input id="i7s12i06" type="submit" value="submit" form="i7s12i04" class="C1_button2" disabled="">				<div id="i7s12i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s12i01" class="C1_text">  We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp  aiiA, sfgfp  aiiA ndh. SDS-PAGE gel pictures are shown as follows.
+</textarea>				<br>			</td>		</tr>	</tbody></table>	</td>		</tr>		</tbody></table>				<div class="M1_exp_stepInfo" title="10.Characterization of several parts using additional AHL .(9.27)" id="sb7s12">			<button class="M1_exp_step_name_button"><input value="10.Characterization of several parts using additional AHL .(9.27)" class="M1_exp_step_name"></button>			<span class="M1_exp_step_time">2013/9/28 08:44:02--Chun Tang</span>		</div>		<table class="M1_exp_stepBoard">		<tbody><tr class="M1_exp_stepItem">			<td class="M1_exp_item_time" id="ib7s12i0">2013/9/28 08:44:22</td>			<td class="M1_exp_item_content">	<table id="i7s12i0" class="C1">		<tbody><tr>			<td class="C1_td">				<img id="i7s12i00" src="http://trysomething-block.stor.sinaapp.com/t14i7s12i0sds1.png"><br>				<form id="i7s12i04" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form>				<input name="img" type="file" id="i7s12i02" form="i7s12i04" class="C1_button1" required="required" disabled="">				<input name="pname" type="hidden" value="t14" form="i7s12i04">				<input name="id" type="hidden" value="i7s12i0" form="i7s12i04">				<input name="oldgraph" type="hidden" id="i7s12i05" form="i7s12i04" value="t14i7s12i0sds1.png">				<input id="i7s12i06" type="submit" value="submit" form="i7s12i04" class="C1_button2" disabled="">				<div id="i7s12i03" class="M1_exp_img"></div>				</td>			<td class="C1_td_text">				<textarea id="i7s12i01" class="C1_text">  We continued to characterize our biobricks using additional AHL. These plasmids included sfgfp, sfgfp-aiiA, sfgfp-aiiA-ndh. SDS-PAGE gel pictures are shown as follows.
 Illustration:

Team:XMU-China/wetlab journal