Team:KU Leuven/Project/Ecological/Background

From 2013.igem.org

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:http://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

Background

You are here!

Wetlab

Experimental data of the BanAphids

Proposed functions of methyl salicylate

When aphids feed on plants, plants will react by emitting herbivore-induced plant volatiles, and these mediate relationships between plants and insects through the attraction of natural enemies and the repulsion of herbivores (Vlot et al., 2008). Aphid feeding will specifically induce a significantly higher production of MeS than any other herbivore-induced plant volatile (Blande et al., 2010). MeS is a volatile phytohormone that is a product of the salicylic acid pathway. Since it is a volatile, MeS can induce defence systems in neighbouring plants as well as itself (Heidel and Baldwin, 2004). In addition to the activation of plant defence systems, MeS has been shown to have another function, it is a potent attractor of natural and effective aphid predators, this includes the seven-spotted ladybug (Coccinella septumpunctata) (Zhu and Park, 2005). Therefore, with the information we have gathered, we expect the BanAphids to be able to attract the aphid's natural predators and activate plant defence mechanisms in order to fend off current and future aphid infestations.


Winged aphid getting ready to migrate (Vilhjalmur Ingi Vilhjalmsson)



Changes in aphid acceptance with MeS treated plants Bars show median aphid settling + standard deviation, plant stages are categorised by the number of leaves. (Ninkovic et al., 2003)

Aphids

Aphids are small, soft-bodied insects that feed on sap from leaves, twigs, or roots. Adult aphids exist in two forms, winged or wingless. They are most common in spring and summer. Under ideal temperatures, many aphid species can complete their life cycle in less than 2 weeks, and because of their prolific reproductive capacity, enormous populations of aphids can build up in a short time.
It is commonly known that when insects damage plants, these plants respond by emitting a range of volatile organic compounds (VOCs) (Blande et al., 2010). Damage caused by chewing herbivores releases a different profile of VOCs than damage by aphid feeding (piercing sucking insects) (Leitner et al., 2005). Blande et al. identified MeS as the most distinctive indicator of aphid feeding in the VOC emission profile. A significant increase in MeS VOC emission was detected from aphid infested plants as well as a significant time effect, meaning that MeS emission intensity increased with the length of the aphid infestation. The effect of aphid feeding on a plant’s MeS induction is immense, even though the feeding pressure due to aphids must exceed a threshold level before inducing volatile emissions. MeS comprises almost two-thirds of the total emission, even after 21 days of aphid feeding, compared to a negligible emission from control plants (Blande et al., 2010).
MeS is an important compound of a plant’s defence mechanisms acting both as an aphid repellent (Glinwood and Pettersson, 2000) and an attractor of natural predators and parasitoids of aphids (Zhu and Park, 2005). In field trials, MeS successfully reduced initial colonisation of cereals by bird cherry-oat aphids (Rhopalosiphum padi) migrants (Pettersson et al., 1994). R. padi alternate between a winter host, bird cherry Prunus padus tree and a summer host, cereals, by means of winged aphid migrants Pettersson et al., 1994). With this and following studies, it has been shown that the principal mode of action against aphids is repellency, which is what we want to achieve with MeS and EBF. The behavioural response of aphids to these substances is increased mobility, reduced reproductive efficiency and increased mortality of adults. An aphid’s response however, appears to be dynamic, losing their negative response to MeS after three or four days of adult life (Glinwood and Pettersson, 1999).
A further study showed that application of MeS significantly reduced the mean aphid numbers in cereal crops by 25-50% (Ninkovic et al., 2003). The immigration and settling of R. padi in barley fields was delayed due to MeS application as well as a significant reduction in maximum aphid densities. Preference tests however showed that the effect of MeS on settling of R. padi on barley decreases with increasing plant age, demonstrating yet again the dynamicity of aphid behavioural responses (Ninkovic et al., 2003).

Predators - prey localisation

Predatory and parasitoid insects have a specialized sensory nervous system to detect their prey (Hatano et al., 2008). They use volatiles produced by the herbivores, reliable but at low concentration, or those produced by the plant to locate their prey. These latter are easily detectable, but are less reliable. To overcome the reliablility-detecability problem predators and parasitoids focus on the responses on stimuli created by specific interactions between the herbivore and its plant (Vet and Dicke, 1992). In response to an aphid attack, plants modify their volatile emissions and these changes are detected by the natural predators of aphids (Du et al., 1998).
Host selection occurs in three phases: habitat localization, host localization and host acceptance (Vinson, 1976). Aphid natural enemies must first locate the aphid habitat, the host plant where aphids are present. Therefore, plant-derived volatiles are used, since evidence of aphid damage is acquired. One of the important herbivore-induced plant volatiles that are used by predators is MeS (Zu & Park, 2005). The feeding of aphids on the plant induces the de novo production of salicylic acid (one of the main components in plant defence systems) which can then be metabolised into MeS (Birkett et al., 2000).
Following habitat localization, natural enemies use short-range physical (colour, shape, movement of aphid) and chemical cues to search for a suitable herbivore on the host plant (Hatano et al., 2008). One of the chemical cues used by natural enemies of aphids is honeydew. Mostly, the natural enemies need physical contact with honeydew to change their behavior (Ide et al., 2007). In addition to aphid honeydew, an aphid's alarm pheromone, EBF, is also an important semiochemical in aphid localization. It is secreted from the cornicle of many aphid species (Franscis et al., 2005) to alert surrounding aphids of the presence of natural enemies (Kunert et al., 2005). Detection of these short-range chemical cues does not lead to the aphid directly, but only indicates its presence, improving prey detection of the predators and parasitoids.
Once an aphid is located, natural enemies have to recognize it as a potential prey before they attack it. For host recognition, non-volatile chemical cues are important, in particular contact with the cornicle wax on the surface of the aphids. Predators use their antennae or their mouthparts to recognize the prey. Parasitoids use probing to assess host quality before oviposition (Hatano et al., 2008). More information about host localisation can be found below in E-β-farnesene, effect on predators.


Ladybug eating aphid

Ladybug has found an aphid. (John Flannery)

Aphid mummy

Aphids attacked by a parasitic wasp larva transform into a mummy and die


Plant communication via MeS (Taiz and Zeigner, 2010)

Plants - defence systems

Since plants are sessile organisms, they have no chance of escaping attacks from herbivores and must use other strategies to defend themselves (Mithöfer and Boland, 2012). A plant’s defence mechanisms can be divided into two broad categories: constitutive and induced defences (Pieterse et al., 2009). Constitutive defences are always present in the plant, while induced defences are produced in response to damage by herbivores or other organisms. Many physical defences and large quantitative defences (eg. Cellulose, tannins) are constitutive, they are usually distributed in permanent woody tissue and are thus difficult to mobilise. Induced defences include toxic chemicals, pathogen-degrading enzymes and physiological changes (Mithöfer and Boland, 2012). Contrary to constitutive defences, induced defences are only produced when needed (Wu and Baldwin, 2009). Therefore, the induced defences are less costly for the plant, especially when the presence of herbivores is variable (Karban et al., 1997).

Biotrophic pathogens, like aphids, colonise living plant tissue and establish a long-term feeding relationship with their hosts, instead of killing the host tissue and extracting the nutrients from the dead host cells like necrotrophs (Jones and Dangl, 2006). Plant resistance to biotrophic pathogens is classically thought to be mediated through salicylic acid signalling (Loake and Grant, 2007). SA, a phenolic phytohormone, is involved in many functions such as mediating in plant defence against pathogens. SA induces the production of pathogenesis-related (PR) proteins and is involved in the systemic acquired resistance (SAR), which is a "whole-plant" resistance response that occurs following an earlier localised exposure to a pathogen (Mauch-Mani and Métraux, 1998). SAR is analogous to the innate immune system found in animals.
The resistance observed following induction of SAR is effective against a wide range of pathogens and is linked to an accumulation of endogenous SA. SA modifications such as methylation and amino acid conjugation provide biological specificity in plant defence responses (Loake and Grant, 2007).
MeS, a volatile ester, is normally absent in plants but is dramatically induced upon pathogen infection, this is an example of an induced defence mechanism. It acts as a mobile inducer of SAR by carrying this ‘under attack’ signal to neighbouring plants. The following hydrolysis of MeS to SA is catalysed by a methyl esterase in it’s immediate surrounding and activates the plant defences.

Plants - optimal defence theory

The distribution of the induced defence chemicals is predicted by the optimal defence theory (McKey, 1974). The optimal defence theory is based on three factors: cost of defence, value of the plant part and risk of attack. First, the plant’s chemical defence has a cost, since the energy spent on defence cannot be used for other functions, such as reproduction and growth. Second, lost plant tissue has a fitness impact, but the impact is dependent on the value of the plant part. Generally, terminal leaves and reproductive parts, seeds in particular, have greater value than the other plant parts. Therefore, these plant parts contain more defences. Finally, not all plant parts are equally likely to be attacked. Plants invest heavily in effective defences in parts that are easily found by herbivores. Plant parts that are more likely to be located by herbivores are parts that have been available longer and parts that consist of more apparent tissues. Hence, the distribution of the defensive compounds within plant parts is highly organized. The optimal defence hypothesis predicts that the plants will allocate more energy towards defence when the benefits of protection outweigh the costs, specifically in situations where there is high herbivore pressure (Zangerl and Bazzaz, 1992).


effect on plantgrowth

Effects of whitefly infestations and SA on pepper growth (Yang et al., 2011)


Relationship between above and below ground hebivory (Figure adapted from Bezemer and van Dam, 2005)

Plants - Above vs below ground herbivory

Induced resistances are mainly regulated by salicylic acid (SA)-, jasmonic acid (JA)- and ethylene (ET)-dependent signalling pathways, which are interconnected by complex signalling networks and cross-talk phenomena (Pieterse et al., 2009). Because most systemic responses are mediated by long-distance signals they can cross the aboveground-belowground border, meaning that aerial parts of the plant with its herbivores can change the resistance status of the roots and vice versa (Heil and Ton, 2008). The resulting patterns are highly complex and difficult to predict (Yang et al., 2011).
Leaves and roots are separated from each other by great distances, nevertheless roots are implicated in aboveground plant-herbivore dynamics (Kaplan et al, 2008). Because roots are surrounded by soil and therefore less susceptible to herbivory, they are an ideal storage site for chemicals used in aboveground defences. This provides a direct link between belowground and aboveground resistance (van der Putten et al., 2001). In addition to providing a safe storing site for defence molecules, roots have also been implicated in tolerance to aboveground herbivores. The roots provide also a temporary storage site for primary metabolic products that would otherwise be vulnerable to the aboveground consumers through an elevation in the sink strength. These resources can then be re-allocated at a later time for aboveground growth and reproduction (Schwachtje et al., 2006).
The production of leaf defences belowground gives root herbivores the chance of interfering with the production and translocation and hence benefit aboveground herbivores by reducing plant resistance (Kaplan et al., 2008). Similarly, if aboveground herbivores elicit a tolerance response whereby plants allocate valued nutritional resources belowground, this storage effect may benefit root herbivores (Schwachtje et al, 2006). In conclusion, herbivore-induced facilitation may be an important feature that links the dynamics of above- and belowground communities (Yang et al., 2011).

Proposed functions of E-β-farnesene

Aphid populations are regulated by natural enemies including ladybugs (Minks and Harrewijn, 1978). For many species of aphids, avoidance of these enemies involves the release of an alarm pheromone E-β-farnesene (EBF) (Xiangyu et al., 2002). EBF is released from the aphid’s cornicles when they are attacked (Dixon, 1998). Therefore, EBF functions as a direct repellent of aphids. In addition, it also and acts as an attractant of their natural enemies (Hatano et al., 2008). Therefore, we expect the BanAphids to be able to repel aphids and on top of that, attract their natural enemies to make sure the aphids are thoroughly removed.


Green peach aphid (Nick Monaghan)

Aphids - Alarm

Aphids are a very primitive insect species but they have developed a unique system to protect themselves from predators and parasitoids. When under attack these soft-bodied, sedentary Homopterans (plant feeders by sucking sap) secrete droplets from cornicles, a specialised paired tubular structure – see aphid biology 101. The essential compound of these secreted droplets has been identified to be E-beta-farnesene (Edwards et al., 1973). EBF causes changes in gene expression and acts as a repellent to other, neighbouring aphids, causing avoidance behaviour. This means that aphids that are normally sedentary will increase movement by walking, falling or jumping away from their feeding sites on plants (Nault et al., 1973). Other aphids use EBF to alert tending ants (see aphid biology 101), which then attack incoming predators such as the ladybug (Nault et al., 1976). EBF has also been shown to significantly prolong developmental times of the aphids as well as lower fertility (Su et al., 2006). De Vos et al. has shown that continuous exposure to EBF lead to habituation within three generations meaning they show no avoidance response anymore. This insensitivity can however be reverted back into being EBF-sensitive in three generations. This shows that EBF is an essential signal to aphids and that an aphid’s response is dynamic, to accommodate this dynamic behavioural response, we have developed an oscillator model. Furthermore, EBF can function as a kairomone, attracting predators and parasitoids (see below).

Predators - Prey localisation

After localising the aphid’s habitat by the use of herbivore-induced plant volatiles (see above), predators must find the exact location of the aphids on the plant. For this purpose, predators use short-range physical (colour, shape, movement of aphid) and chemical cues (Hatano et al., 2008). One of the chemical cues used by natural enemies of aphids is honeydew. Mostly, the natural enemies need physical contact with honeydew to change their behavior (Ide et al., 2007). In addition to aphid honeydew, the aphid alarm pheromone EBF is also an important semiochemical in aphid localisation. It is secreted from the cornicle of many aphid species (Franscis et al., 2005) to alert surrounding aphids of the presence of natural enemies (Kunert et al., 2005). Predators have specific neuronal receptors to detect pheromones emitted by their prey. Various aphid natural enemies, including Adalia bipunctata, showed fast and pronounced orientation behaviour toward the EBF source in an olfactometer bioassay (Francis et al., 2004).


Adalia

Adalia bipunctata (Jon Law)

Berlandier Françoise, Severtson Dusty and Mangano Peter.(2010) Aphid management in canola crops. Farmnote 45/2004
Bezemer, T.M., and van Dam, N.M. (2005). Linking aboveground and belowground interactions via induced plant defenses. Trends in Ecology and Evolution 20:617-624
Birkett, M.A., Campbell, C.A.M., Chamberlain, K., Guerrieri, E., Hick, A.J., Martin, J.L., Matthes, M., Napier, J.A., Pettersson, J., Pickett, J.A., Poppy, G.M., Pow, E.M., Pye, B.J., Smart, L.E., Wadhams, G.H., Wadhams, L.J. and Woodcock, C.M. (2000). New roles for cis-jasmone as an insect semiochemicals and in plant defense. PNAS 97:9329-9334
Blackman, R.L., and Eastop, V.F. (1985). Aphids of the world crops: an identification guide. Wiley, Chichester, United Kingdom
Blande, J.D., Korjus, M., and Holopainen, J.K. (2010). Foliar methyl salicylate emissions indicate prolonged aphid infestation on silver birch and black alder. Tree Physiology 30:404–416
Chen, M. (2008). Inducible direct plant defense against insect herbivores a review. Insect Sci. 15:101–114
Cyr, H., and Pace, M.L. (1993). Magnitude and patterns of herbivory un aquatic and terrestrial ecosystems. Nature 361, 148-150
Daly, H., Doyen, J.T. and Purcell, A.H. III (1998) Introduction to insect biology and diversity, 2nd edition. Oxford University Press. New York, New York. Chapter 14, Pages 279-300.
Dixon, A.F.G. (1973). Biology of aphids. Edward Arnold, London, United Kingdom
Du, Y.J., Poppy, G.M., Powell, W., Pickett, J.A., Wadhams, L.J., and Woodcock, C.M. (1998). Identification of semiochemicals released during aphid feeding that attract parasitoid Aphidiuservi. J. Chem. Ecol. 24:1355-1368
Edwards, L.J., Siddall, J.B., Dunham, L.L., Uden, P. & Kislow C. J. (1973). Trans-beta-farnesene, Alarm pheromone of the green peach aphid, Myzus persicae (Sulzer). J. Econ. Entomol. Oct, 99(5): 1636-40
Fox, L.R. (1981). Defence and dynamics in plant-herbivore systems. Amer. Zool. 21:853-864
Francis, F., Martin, T., Lognay, G., and Haubruge, E. (2005).Role of (E)-b-farnesene in systematic aphid prey location by Episyrphusbalteatus larvae (Diptera: Syrphidae). European J. of Entomol., 102:431-436
Francis, F.,Longnay, G., and Haubruge, E. (2004). Olfactory responses to aphid and host plant volatile releases: E-β-Farnesene an effective kairomone for the predator Adalia bipunctata. J. Chem. Ecol. 30:741–755
Glinwood, R.T., and Pettersson, J. (2000). Change in response of Rhopalosiphumpadi spring migrants to the repellent winterhost component methyl salicylate. Entomol. Exp. Appl. 94:325–330
Hatano, E., Kunert, G., Michaud, J.P., and Weisser, W.W. (2008). Chemical cues mediating aphid location by natural enemies. Eur. J. Entomol. 105:797-806
Heil, M., and Ton, J. (2008). Long-distance signaling in plant defence. Trends in Plant Science 13:264–272 Ide, T., Suzuki, N., and Katayama, N. (2007).The use of honeydew in foraging for aphids by larvae of the ladybird beetle, Coccinellaseptempunctata L. (Coleoptera: Coccinellidae). Ecol. Entomol. 32:455-460
Jones, J.D.G., ad Dangl, J.L. (2006). The plant immune system. Nature 444:323-329
Kaplan, I., Halitschke, R., Kessler, A., Rehill, B.J., Sardanelli, S., and Denno, R.F. (2008). Physiological integration of roots and shoots in plant defence strategies links above- and belowground herbivory. Ecol. Letters 11:841-851
Karban, R., Anurag, A.A., and Mangel, M. (1997). The benefits of induced defences against herbivores. Ecology 78:1351-1355
Kunert, G., Otto, S., Röse, S.R., Gershenzon, J., and Weisser, W.W. (2005). Alarm pheromones mediates production of winged dispersal morphs in aphids. Ecology letters, 8:596-603
Lambers, D. H. R., and Schepers, A. (1978).Effect of trans-beta-farnesene, used as a repellant against landing aphid alatae in seed potato growing. Potato Res. 21:23-26
Leitner, M., Boland, W., and Mithöfer, A. (2005). Direct and indirect defences induced by piercing-sucking and chewing herbivores in Medigacotruncatula. New Phytol. 167:597–606
Loake, G., Grant, M. (2007). Salicylic acid in plant defence—the players and protagonists. Current Opinion in Plant Biology 2007, 10:466–472
McKey, D. (1974). Adaptive patterns in alkaloid physiology. Am. Nat. 108:305-320
Minks, A.K. and Harrewijn, P. (1987). Aphids: Their biology, natural enemies and control. Elsevier Science Publishers B.V., Amsterdam, The Netherlands
Mithöfer A., and Boland, W. (2012). Plant defence against herbivores: chemical aspects. Annu. Rev. Plant Biol. 63:431-450
Mithöfer, A., Boland, W., and Maffei, M.E. (2009). Chemical ecology of plant-insect interactions. In: Parker, J., Plant disease resistance. Wiley-Blackwell, Chichester
Ninkovic, V., Ahmed, E., Glinwood, R., and Pettersson, J. (2003) Effects of two types of semiochemicals on population development of the Bird Cherry Oat Aphid, Rhopalosiphumpadi (L.) a in barely crop. Agricultural and Forest Entomology 5: 27-33
Pettersson, J., Pickett, J.A., Pye, B.J., Quiroz, A., Smart, L.E., Wadhams, L.J., and Woodcock, C.M. (1994).Winter host component reduces colonisation by bird cherry-oat aphid, Rhopalosiphumpadi (L.) (Homoptera, Aphididae), and other aphids in cereal fields. J. Chem. Ecol. 20:2565–2574
Pieterse, C.M., Leon-Reyes, A., Van der Ent, S., and Van Wees, S.C. (2009). Networking by small-molecule hormones in plant immunity. Nature Chemical Biology 5:308–316
Rhoades, D. F., and R. G. Cates. 1976. Towards a general theory of plant antiherbivore chemistry. Recent Adv. Phytochem. 10:168-213
Schwachtje, J., Minchin, P.E.H., Jahnke, S., van Dongen, J.T., Schittko, U., and Baldwin, I.T. (2006). SNF1-related kinases allow plants to tolerate herbivory by allocating carbon to roots. Proc. Natl. Acad. Sci. U S A. 103:12935–12940
Taiz, L., and Zeiger, E. (2010). Plant physiology. Sinauer Associates, Sunderland
van der Putten, W.H., Vet, L.E.M., Harvey, J.A., and Wäckers, F.L. (2001). Linking above- and belowground multitrophic interactions of plants, herbivores, pathogens, and their antagonists. Trends Ecol. Evol. 16:547–554
Vet, L.E.M., and Dicke, M. (1992).Ecology of infochemical use by natural enemies in a tritrophic context. Annu. Rev. Entomol. 37:141-172
Vinson, S.B. (1976). Host selection by insect parasitoids. Annu. Rev. Entomol. 21:109-133
Wu, J., and Baldwin, I.T. (2009). Herbivory-induced signalling in plants: perception and action. Plant Cell Environ. 32:1161-1174
Xiangyu, J.G., Zhang, F., Fang, Y.L., Kan, W., Zhang, G.X., and Zhang, Z.N. (2002). Behavioural response of aphids to the alarm pheromone component (E)-beta-farnesene in the field. Physiological Entomology 27:307–311
Yang, J.W., YI, H.-S., Kim, H., Lee, B., Lee, S., Ghim, S.-Y., and Ryu, C.-M. (2011). Whitefly infestation of pepper plants elicits defence responses against bacterial pathogens in leaves and roots and changes the below-ground microflora. J. of Ecol. 99:46-56
Zangerl, A.R., and Bazzaz, F.A. (1992). Theory and pattern in plant defence allocation. In: Frits, R.S., and Simms, E.L., Plant resistance to herbivores and pathogens: ecology, evolution, and genetics. The Univ. of Chicago Press, Chicago
Zhu, J.W., and Park, K.C. (2005). Methyl salicylate, a soybean aphid-induced plant volatile attractive to the predator Coccinella septempunctata. J. Chem. Ecol. 31:1733–1746