Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 19th July.html

From 2013.igem.org

Trojan Horse

19th July


Conjugation results :
Controls => no colonies
Mix = tapis
Ie worked really well.
To be sure to start only with one clone => restreak some colonies on tet/spec plates

Transformation by electroporation : goal = get a phage factory.
We do it three different ways in order to see which one works (best).
Transform strain containing helper plasmid with phagemid
Transform strain containing helper plasmid with phagemid
Co Transform strain with both plasmids at the same time

From strain sT004, sT005, sT006
Transform sT004 with pT006
Transform sT005 with pT005
Co Transform sT006 with pT005 and pT006

Tubes :
  1. sM13KO7 (control) : 5,60
  2. sM13KO7 + pLitmus : 5,70
  3. sM13KO7 + pLitmus : 5,70
  4. sLITMUS (control) : 5,60
  5. sLITMUS + pM13K07 : 5,60
  6. sLITMUS + pM13K07 : 5,60
  7. MG16165 : control = 5.70
  8. MG16165 + p (M13K07) and p(LITMUS) = 5.60
  9. MG16165 + p (M13K07) and p(LITMUS)= 5.60

Recovery 2h

Plate everything on Kan/ Amp

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